This README accompanies data_fragmentation_T.crassispinus.xlsx Associate publication : Fragmentation shapes nest density and social structure but not genetic diversity of Temnothorax crassispinus (Formicidae) M. Cordonnier, T. Lindner, J. Heinze Lehrstuhl für Zoologie / Evolutionsbiologie, Univ. Regensburg ****************************** CONTENTS ******************************* The data can be readily imported in any statistical package or spreadsheet program. Please, contact me if you need the file formatted in other ways. This file includes a description of the variables. *********************************************************************** Variable names and descriptions Sheet 1 ID nest: identity of the nest of the genotyped worker 2MS46 to GT-1: complete genotype of the worker. Missing data: “-9” Q1_inter & Q2_inter: Q-values resulting from the Bayesian clustering (interspecific level) ID_inter: species identity based on genotype (TC: Temnothorax crassispinus, TN: T. nylanderi; H: intermediate) Q1_intra & Q2_intra: Q-values resulting from the Bayesian clustering (intraspecific level) ID_intra: population identity based on genotype (TC1: Temnothorax crassispinus, cluster1; TC2: T. crassispinus, cluster2) ID_sequencing_C1-J-2183/C2-N-3661: species identity based on sequencing for primers C1-J-2183/C2-N-3661 (TC: Temnothorax crassispinus, TN: T. nylanderi) ID_sequencing_LCO1490 ⁄ HCO2198: species identity based on sequencing for primers LCO1490 ⁄ HCO2198 (TC: Temnothorax crassispinus, TN: T. nylanderi) Sheet 2 Idpatch: identity of the forest patch Latitude, longitude: geographic coordinates of the forest patch Date, Hour: Sampling time of the forest patch TC_Nest-density: number of T. crassispinus nests sampled per people in 30 minutes in the forest patch Prop_Queenright_nests: proportion of queenright nests in the forest patch Gen_div: averaged number of alleles per T. crassispinus nest in the forest patch Connect1 & Connect5: distance to the closest patch and mean distance to the five closest patches Nbneig200 & Nbneig400: number of neighboring forest patches at 200 and 400 meters from the focal forest patch area: size of the forest patch shape: shape of the forest patch (perimeter/surface) ****************************** CONTACTING ***************************** Contact me at: Marion Cordonnier e-mail: [email protected] ***********************************************************************

This README accompanies data_fragmentation_T.crassispinus.xlsx Associate publication : Fragmentation shapes nest density and social structure but not genetic diversity of Temnothorax crassispinus (Formicidae) M. Cordonnier, T. Lindner, J. Heinze Lehrstuhl für Zoologie / Evolutionsbiologie, Univ. Regensburg ****************************** CONTENTS ******************************* The data can be readily imported in any statistical package or spreadsheet program. Please, contact me if you need the file formatted in other ways. This file includes a description of the variables. *********************************************************************** Variable names and descriptions Sheet 1 ID nest: identity of the nest of the genotyped worker 2MS46 to GT-1: complete genotype of the worker. Missing data: “-9” Q1_inter & Q2_inter: Q-values resulting from the Bayesian clustering (interspecific level) ID_inter: species identity based on genotype (TC: Temnothorax crassispinus, TN: T. nylanderi; H: intermediate) Q1_intra & Q2_intra: Q-values resulting from the Bayesian clustering (intraspecific level) ID_intra: population identity based on genotype (TC1: Temnothorax crassispinus, cluster1; TC2: T. crassispinus, cluster2) ID_sequencing_C1-J-2183/C2-N-3661: species identity based on sequencing for primers C1-J-2183/C2-N-3661 (TC: Temnothorax crassispinus, TN: T. nylanderi) ID_sequencing_LCO1490 ⁄ HCO2198: species identity based on sequencing for primers LCO1490 ⁄ HCO2198 (TC: Temnothorax crassispinus, TN: T. nylanderi) Sheet 2 Idpatch: identity of the forest patch Latitude, longitude: geographic coordinates of the forest patch Date, Hour: Sampling time of the forest patch TC_Nest-density: number of T. crassispinus nests sampled per people in 30 minutes in the forest patch Prop_Queenright_nests: proportion of queenright nests in the forest patch Gen_div: averaged number of alleles per T. crassispinus nest in the forest patch Connect1 & Connect5: distance to the closest patch and mean distance to the five closest patches Nbneig200 & Nbneig400: number of neighboring forest patches at 200 and 400 meters from the focal forest patch area: size of the forest patch shape: shape of the forest patch (perimeter/surface) ****************************** CONTACTING ***************************** Contact me at: Marion Cordonnier e-mail: [email protected] ***********************************************************************

This README accompanies data_genotyping.txt Associate publication : Absence of genetic isolation across highly fragmented landscape in the ant Temnothorax nigriceps M. Cordonnier^a, D. Felten^a, A. Trindl^a, J. Heinze^a, A. Bernadou^{a a}Lehrstuhl für Zoologie / Evolutionsbiologie, Univ. Regensburg *Equal contribution ****************************** CONTENTS ******************************* The data can be readily imported in any statistical package or spreadsheet program. Please, contact me if you need the file formatted in other ways. This file includes a description of the variables. *********************************************************************** Variable names and descriptions Sample: ID of the sampled nest Location: Population of the sampled nest List of genotypes Microsatellite primers used in the study Annealing temperature [°C] Orientation Sequence of primers LX GT218 57 Forward 5’-GTTCTTGCGCGGATGCATAC-3’ Reverse 5’-TGTACTCGCGTGTCTATCGG-3’ Ant3993 57 Forward 5’-TGATCCGCTCTTAAAATTTAGATGGA-3’ Reverse 5’-ACTTTCCGCRGCATTAAACATTTTCTT-3’ L-18 57 Forward 5’-TGAATTTGGATGGCGGTAGAC-3’ Reverse 5’-ACCTAATGCACGCTTTAGAAT-3’ LXA GT1 57 Forward 5’-GTGGCGACCAATTCTGCAAG-3’ Reverse 5’-GCAGGACCAGCATCAAATGACAG-3’ 2MS17 55 Forward 5’-CAGCCTCTATTTTGTTCGAAG-3’ Reverse 5’-TTTACTGCGGCTCCATAATC-3’ 2MS46 55 Forward 5’-GCTCACTACTATGCTGCCAGC-3’ Reverse 5’-CTTTCCTGCAAACCACGTGT-3’ 2MS60 55 Forward 5’-TATGCGCCGGACAATAATCGC-3’ Reverse 5’-GTTCATTGTCCGAGGCGCAGC-3’ 2MS67 55 Forward 5’-GAAGATTCGTCAGGATGCAGC-3’ Reverse 5’-AACTCTCGCTGGCAAGCGAGC-3’ 2MS82 55 Forward 5’-AAAAGAGCATGCAACAGGTCAGC-3’ Reverse 5’-TTTCTTAAGTCGCAAGCGAGC-3’ 2MS87 55 Forward 5’-GGAACCTCACTCAACCTCGGT-3’ Reverse 5’-ACGCGGACTACTTTAACCGGA-3’ 2MS91 55 Forward 5’-AAAGTCTCGGAGTGGCTTTGC-3’ Reverse 5’-ATTCTCGTCCATTTGTTCTAA-3’ Ant11893 55 Forward 5’-CAGGCTCGGRACGTTAATGC-3’ Reverse 5’-GGTGCCGACGTCTAGCTAGC-3’ Missing data are encoded “-9”. ****************************** CONTACTING ***************************** Contact me at: Marion Cordonnier e-mail: [email protected] ***********************************************************************

This README accompanies data_genotyping.txt Associate publication : Absence of genetic isolation across highly fragmented landscape in the ant Temnothorax nigriceps M. Cordonnier^a, D. Felten^a, A. Trindl^a, J. Heinze^a, A. Bernadou^{a a}Lehrstuhl für Zoologie / Evolutionsbiologie, Univ. Regensburg *Equal contribution ****************************** CONTENTS ******************************* The data can be readily imported in any statistical package or spreadsheet program. Please, contact me if you need the file formatted in other ways. This file includes a description of the variables. *********************************************************************** Variable names and descriptions Sample: ID of the sampled nest Location: Population of the sampled nest List of genotypes Microsatellite primers used in the study Annealing temperature [°C] Orientation Sequence of primers LX GT218 57 Forward 5’-GTTCTTGCGCGGATGCATAC-3’ Reverse 5’-TGTACTCGCGTGTCTATCGG-3’ Ant3993 57 Forward 5’-TGATCCGCTCTTAAAATTTAGATGGA-3’ Reverse 5’-ACTTTCCGCRGCATTAAACATTTTCTT-3’ L-18 57 Forward 5’-TGAATTTGGATGGCGGTAGAC-3’ Reverse 5’-ACCTAATGCACGCTTTAGAAT-3’ LXA GT1 57 Forward 5’-GTGGCGACCAATTCTGCAAG-3’ Reverse 5’-GCAGGACCAGCATCAAATGACAG-3’ 2MS17 55 Forward 5’-CAGCCTCTATTTTGTTCGAAG-3’ Reverse 5’-TTTACTGCGGCTCCATAATC-3’ 2MS46 55 Forward 5’-GCTCACTACTATGCTGCCAGC-3’ Reverse 5’-CTTTCCTGCAAACCACGTGT-3’ 2MS60 55 Forward 5’-TATGCGCCGGACAATAATCGC-3’ Reverse 5’-GTTCATTGTCCGAGGCGCAGC-3’ 2MS67 55 Forward 5’-GAAGATTCGTCAGGATGCAGC-3’ Reverse 5’-AACTCTCGCTGGCAAGCGAGC-3’ 2MS82 55 Forward 5’-AAAAGAGCATGCAACAGGTCAGC-3’ Reverse 5’-TTTCTTAAGTCGCAAGCGAGC-3’ 2MS87 55 Forward 5’-GGAACCTCACTCAACCTCGGT-3’ Reverse 5’-ACGCGGACTACTTTAACCGGA-3’ 2MS91 55 Forward 5’-AAAGTCTCGGAGTGGCTTTGC-3’ Reverse 5’-ATTCTCGTCCATTTGTTCTAA-3’ Ant11893 55 Forward 5’-CAGGCTCGGRACGTTAATGC-3’ Reverse 5’-GGTGCCGACGTCTAGCTAGC-3’ Missing data are encoded “-9”. ****************************** CONTACTING ***************************** Contact me at: Marion Cordonnier e-mail: [email protected] ***********************************************************************