Automated Organization ProfileEMBL Australia Node in Single Molecule Science, School of Biomedical Sciences, University of New South Wales, Sydney, Australia
EMBL Australia Node in Single Molecule Science, School of Biomedical Sciences, University of New South Wales, Sydney, Australia
Current S-Index
Sum of Dataset Indices for all datasets
Average Dataset Index per Dataset
Average Dataset Index per dataset
Total Datasets
Total datasets in this organization
Average FAIR Score
Average FAIR Score per dataset
Total Citations
Total citations to the organization's datasets
Total Mentions
Total mentions of the organization's datasets
S-Index Interpretation
The S-Index (Sharing Index) is a comprehensive metric that represents the cumulative impact of all your datasets. It is calculated as the sum of Dataset Index scores across all your claimed datasets.
What it means:
- A higher S-index indicates greater overall impact of your datasets relative to typical datasets in their fields of research
- The S-Index grows as you add more datasets or as existing datasets gain more citations and mentions
- It provides a single number to track your research data impact over time
Current S-Index: 2.3 (sum of 2 datasets Dataset Index scores)
More information here.
S-Index Over Time
Cumulative Citations Over Time
Cumulative Mentions Over Time
Datasets
eSRRF supplementary data The eSRRF preprint is available here: https://doi.org/10.1101/2022.04.07.487490 Find the code and documentation here: https://github.com/HenriquesLab/NanoJ-eSRRF 1a: Set01a_DNA-PAINT_COS7-MT.tif DNA-PAINT of fixed COS-7 cells with indirect immunolabeling against tubulin Pixelsize: 160 nm Exposure time: 30 ms 50 000 frames 1b: Set01b_DNA-PAINT_COS7-MT_bin100.tif Dataset 1a with 100 frames binning to effectivly increase emitter density. eSRRF analysis parameters: M=5, R=0.5, S=1 all frames 2: Set02_HeLa_ffDronpa-MAP4.tif Live-cell TIRF imaging dataset of HeLa cells expressing ffDronpa-MAP4 Pixelsize: 156 nm Exposure time: 40 ms eSRRF analysis parameters: M=4 R=2 S=4 100 frames rolling analysis gap = 50 frames 3: Set03_COS7_PrSS-mEmerald-KDEL.tif Live-cell HiLO-TIRF of COS-7 cells expressing PrSS-mEmerald-KDEL marking the ER lumen. Pixelsize: 160 nm Exposure time: 5 ms eSRRF analysis parameters: M=5 R=2 S=1 100 fr rolling analysis gap = 10 frames 4a: Set4a_MFM_HeLa_TOM20.tif Live-cell MFM of HeLa cells expressing TOM20-HaloTag labeled with JF549 Pixelsize: 160 nm Exposure time: 20 ms eSRRF analysis parameters: M=4 R=2 S=1 100 fr rolling analysis gap = 25 frames 3D axial offset = 390 nm 4b: Set4b_MFM_beadcalibration.tif MFM calibration data with TetraSpeck beads immobilized on a coverslip, Pixelsize: 160 nm z-step 60 nm 5: Set5_LLS_Jurkat_ER.zip Lattice light sheet (LLS) data of the ER in Jurkat cells. The zip-folder contains single image stacks for each z-plane: 98 z-planes with 100 fr per plane at 10 ms exposure time 276 nm step size in the z-axis and 104 nm pixelsize. Each z-plane stack was process with eSRRF followed by deskewing with the LLSM plugin (https://monash-merc.github.io/llsm/docs/fiji/index.html) to recover a single 3D volume reconstruction. eSRRF analysis parameters: M=5 R=3.5 S=2 100 frames 6: Set6_TIRF_NeuronCulture_MT_Skylan-NS.tif TIRF imaging data of cultured neuron expressing Skylan-NS tagged tubulin. Pixelsize: 64.7 nm Exposure time: 100 ms eSRRF analysis parameters: M=4 R=5 S=2 200 fr 7: Set7_SDC_U2OS_Skylan-S-Actin.tif Live-cell SDC imaging data of U2OS cells transiently expressing SkylanS-ßActin over a time course of 12 hours by acquiring substacks of 50 frames at 10 min intervalls. Pixelsize: 247.6 nm Exposure time: 500ms eSRRF analysis parameters: M=5 R=2.5 S=1 50 frames
Authors
- Laine, Romain F. ;
- Heil, Hannah S. ;
- Coelho, Simao ;
- Nixon-Abell, Jonathon ;
- Jimenez, Angélique ;
- Wiesner, Theresa ;
- Martínez, Damián ;
- Galgani, Tommaso ;
- Régnier, Louise ;
- Stubb, Aki ;
- Follain, Gautier ;
- Webster, Samantha ;
- Goyette, Jesse ;
- Dauphin, Aurelien ;
- Salles, Audrey ;
- Culley, Siân ;
- Jacquemet, Guillaume ;
- Hajj, Bassam ;
- Leterrier, Christophe ;
- Henriques, Ricardo
eSRRF supplementary data The eSRRF preprint is available here: https://doi.org/10.1101/2022.04.07.487490 Find the code and documentation here: https://github.com/HenriquesLab/NanoJ-eSRRF 1a: Set01a_DNA-PAINT_COS7-MT.tif DNA-PAINT of fixed COS-7 cells with indirect immunolabeling against tubulin Pixelsize: 160 nm Exposure time: 30 ms 50 000 frames 1b: Set01b_DNA-PAINT_COS7-MT_bin100.tif Dataset 1a with 100 frames binning to effectivly increase emitter density. eSRRF analysis parameters: M=5, R=0.5, S=1 all frames 2: Set02_HeLa_ffDronpa-MAP4.tif Live-cell TIRF imaging dataset of HeLa cells expressing ffDronpa-MAP4 Pixelsize: 156 nm Exposure time: 40 ms eSRRF analysis parameters: M=4 R=2 S=4 100 frames rolling analysis gap = 50 frames 3: Set03_COS7_PrSS-mEmerald-KDEL.tif Live-cell HiLO-TIRF of COS-7 cells expressing PrSS-mEmerald-KDEL marking the ER lumen. Pixelsize: 160 nm Exposure time: 5 ms eSRRF analysis parameters: M=5 R=2 S=1 100 fr rolling analysis gap = 10 frames 4a: Set4a_MFM_HeLa_TOM20.tif Live-cell MFM of HeLa cells expressing TOM20-HaloTag labeled with JF549 Pixelsize: 160 nm Exposure time: 20 ms eSRRF analysis parameters: M=4 R=2 S=1 100 fr rolling analysis gap = 25 frames 3D axial offset = 390 nm 4b: Set4b_MFM_beadcalibration.tif MFM calibration data with TetraSpeck beads immobilized on a coverslip, Pixelsize: 160 nm z-step 60 nm 5: Set5_LLS_Jurkat_ER.zip Lattice light sheet (LLS) data of the ER in Jurkat cells. The zip-folder contains single image stacks for each z-plane: 98 z-planes with 100 fr per plane at 10 ms exposure time 276 nm step size in the z-axis and 104 nm pixelsize. Each z-plane stack was process with eSRRF followed by deskewing with the LLSM plugin (https://monash-merc.github.io/llsm/docs/fiji/index.html) to recover a single 3D volume reconstruction. eSRRF analysis parameters: M=5 R=3.5 S=2 100 frames 6: Set6_TIRF_NeuronCulture_MT_Skylan-NS.tif TIRF imaging data of cultured neuron expressing Skylan-NS tagged tubulin. Pixelsize: 64.7 nm Exposure time: 100 ms eSRRF analysis parameters: M=4 R=5 S=2 200 fr 7: Set7_SDC_U2OS_Skylan-S-Actin.tif Live-cell SDC imaging data of U2OS cells transiently expressing SkylanS-ßActin over a time course of 12 hours by acquiring substacks of 50 frames at 10 min intervalls. Pixelsize: 247.6 nm Exposure time: 500ms eSRRF analysis parameters: M=5 R=2.5 S=1 50 frames
Authors
- Laine, Romain F. ;
- Heil, Hannah S. ;
- Coelho, Simao ;
- Nixon-Abell, Jonathon ;
- Jimenez, Angélique ;
- Wiesner, Theresa ;
- Martínez, Damián ;
- Galgani, Tommaso ;
- Régnier, Louise ;
- Stubb, Aki ;
- Follain, Gautier ;
- Webster, Samantha ;
- Goyette, Jesse ;
- Dauphin, Aurelien ;
- Salles, Audrey ;
- Culley, Siân ;
- Jacquemet, Guillaume ;
- Hajj, Bassam ;
- Leterrier, Christophe ;
- Henriques, Ricardo