Our proteomics dataset comes from The PRoteomics IDEntifications (PRIDE) database, the world’s largest data repository of mass spectrometry-based proteomics data. Specifically, we used 633 human proteomics project experiments with a total of 32,546 runs and reanalyzed them using ionbot with an FDR threshold of 0.01 [16], resulting in a total of 154,885,151 peptide spectrum matches for 18,846 proteins. Here is the full list of projects, runs, and general statistics.

Our proteomics dataset comes from The PRoteomics IDEntifications (PRIDE) database, the world’s largest data repository of mass spectrometry-based proteomics data. Specifically, we used 633 human proteomics project experiments with a total of 32,546 runs and reanalyzed them using ionbot with an FDR threshold of 0.01 [16], resulting in a total of 154,885,151 peptide spectrum matches for 18,846 proteins. Here is the full list of projects, runs, and general statistics.

Combined network from scRNA-seq and proteomics data Given the complementary nature of the networks based on scRNA-seq and proteomics data individually, we decided to combine them into a single network. As the Pearson Correlation Coefficient scores from FAVA cannot be assumed to be directly comparable across the two networks, we converted them to probabilistic scores based on the KEGG benchmarks. These calibrated scores were then combined to produce a single network based on scRNA-seq as well as proteomics data. As should be expected, this network outperforms the individual networks, combining the best aspects of both.

Combined network from scRNA-seq and proteomics data Given the complementary nature of the networks based on scRNA-seq and proteomics data individually, we decided to combine them into a single network. As the Pearson Correlation Coefficient scores from FAVA cannot be assumed to be directly comparable across the two networks, we converted them to probabilistic scores based on the KEGG benchmarks. These calibrated scores were then combined to produce a single network based on scRNA-seq as well as proteomics data. As should be expected, this network outperforms the individual networks, combining the best aspects of both.

Combined network from scRNA-seq and proteomics data Given the complementary nature of the networks based on scRNA-seq and proteomics data individually, we decided to combine them into a single network. As the Pearson Correlation Coefficient scores from FAVA cannot be assumed to be directly comparable across the two networks, we converted them to probabilistic scores based on the KEGG benchmarks. These calibrated scores were then combined to produce a single network based on scRNA-seq as well as proteomics data. As should be expected, this network outperforms the individual networks, combining the best aspects of both.