This repository contains code and the data for reproducing results and figures in the associated manuscript: Roving methyltransferases generate a mosaic epigenetic landscape and influence evolution in Bacteroides fragilis group BFG-Analysis-main/ includes scripts and data to process Nanopore and Illumina reads, assemble BFG genomes, polish those genomes, and correct out-of-frame ORFs for MLST alignment. This also includes GenBank reference genomes referred to in the manuscript. tree_files/ includes pylogenetic tree files and aligned sequence files used in Figures 1, 5, and 6 acessory_regions/ includes a .fasta file of accessory regions in each genome from the study in which it was possible to calculate this (using Ppanggolin/panRGP) genomes/ contains different versions of BFG genomes with and without different types of polishing and frame-correction: genomes/pacbio_uncorrected/ contains genomes sequenced with PacBio and assembled with PacBio software Analyzed for MLST trees in Figures: 1, 5, 6 Analyzed in Figures: 5, 6, S7, S9 - S16 genomes/nanopore_racon_medaka/ contains genomes sequenced with Nanopore, assembled with Flye, then polished with racon and medaka. Analyzed in Figures: 5, 6, S7, S9 - S16 genomes/nanopore_racon_medaka_pilon/ contains genomes sequenced with Nanopore, assembled with Flye, polished with racon and medaka, then polished with Illumina reads with pilon. Analyzed in Figures: 5, 6, S7, S9 - S16 genomes/proovframe_BFG_genomes/ contains genomes from the pacbio_uncorrected/, nanopore_racon_medaka/, and nanopore_racon_medaka_pilon/ directories that were frame-corrected with Proovframe. Analyzed for Figures: 2, 3, 4, S2, S3, S4, S5, S6, S8 genomes/nanopore_MEGAN_corrected/ contains genomes from the nanopore_racon_medaka/ and nanopore_racon_medaka_pilon/ directories that were frame-corrected with MEGAN Analyzed for MLST trees in Figures: 1, 5, 6 nanodisco_difference_files/ contains Nanodisco intermediate files reporting the difference in nanopore signal between native and PCR-generated gDNA at each genomic position. They refer to the genomes in directories genomes/nanopore_racon_medaka_pilon/, genomes/nanopore_racon_medaka/, genomes/pacbio_uncorrected/. Each isolate has a genome in only one of these directories. acessory_regions/ has a .fasta file of accessory sequences (per methods in manuscript) of relevant genomes.

This repository contains code and the data for reproducing results and figures in the associated manuscript: Roving methyltransferases generate a mosaic epigenetic landscape and influence evolution in Bacteroides fragilis group BFG-Analysis-main/ includes scripts and data to process Nanopore and Illumina reads, assemble BFG genomes, polish those genomes, and correct out-of-frame ORFs for MLST alignment. This also includes GenBank reference genomes referred to in the manuscript. tree_files/ includes pylogenetic tree files and aligned sequence files used in Figures 1, 5, and 6 acessory_regions/ includes a .fasta file of accessory regions in each genome from the study in which it was possible to calculate this (using Ppanggolin/panRGP) genomes/ contains different versions of BFG genomes with and without different types of polishing and frame-correction: genomes/pacbio_uncorrected/ contains genomes sequenced with PacBio and assembled with PacBio software Analyzed for MLST trees in Figures: 1, 5, 6 Analyzed in Figures: 5, 6, S7, S9 - S16 genomes/nanopore_racon_medaka/ contains genomes sequenced with Nanopore, assembled with Flye, then polished with racon and medaka. Analyzed in Figures: 5, 6, S7, S9 - S16 genomes/nanopore_racon_medaka_pilon/ contains genomes sequenced with Nanopore, assembled with Flye, polished with racon and medaka, then polished with Illumina reads with pilon. Analyzed in Figures: 5, 6, S7, S9 - S16 genomes/proovframe_BFG_genomes/ contains genomes from the pacbio_uncorrected/, nanopore_racon_medaka/, and nanopore_racon_medaka_pilon/ directories that were frame-corrected with Proovframe. Analyzed for Figures: 2, 3, 4, S2, S3, S4, S5, S6, S8 genomes/nanopore_MEGAN_corrected/ contains genomes from the nanopore_racon_medaka/ and nanopore_racon_medaka_pilon/ directories that were frame-corrected with MEGAN Analyzed for MLST trees in Figures: 1, 5, 6 nanodisco_difference_files/ contains Nanodisco intermediate files reporting the difference in nanopore signal between native and PCR-generated gDNA at each genomic position. They refer to the genomes in directories genomes/nanopore_racon_medaka_pilon/, genomes/nanopore_racon_medaka/, genomes/pacbio_uncorrected/. Each isolate has a genome in only one of these directories. acessory_regions/ has a .fasta file of accessory sequences (per methods in manuscript) of relevant genomes.

This repository contains the data for reproducing results and figures in the associated manuscript: Roving methyltransferases generate a mosaic epigenetic landscape and influence evolution in Bacteroides fragilis group Specifically, these R data structures map to genome assemblies from this study, summarizing the difference in current disturbance between native and PCR-amplified genomic DNA through a nanopore. See https://doi.org/10.5281/zenodo.7407113 for corresponding genomes in sub-directories genomes/pacbio_uncorrected/, genomes/nanopore_racon_medaka/, genomes/nanopore_racon_medaka_pilon/.

This repository contains the data for reproducing results and figures in the associated manuscript: Roving methyltransferases generate a mosaic epigenetic landscape and influence evolution in Bacteroides fragilis group Specifically, these R data structures map to genome assemblies from this study, summarizing the difference in current disturbance between native and PCR-amplified genomic DNA through a nanopore. See https://doi.org/10.5281/zenodo.7407113 for corresponding genomes in sub-directories genomes/pacbio_uncorrected/, genomes/nanopore_racon_medaka/, genomes/nanopore_racon_medaka_pilon/.

This repository contains code and the data for reproducing results and figures in the associated manuscript: Roving methyltransferases generate a mosaic epigenetic landscape and influence evolution in Bacteroides fragilis group BFG-Analysis-main/ includes scripts and data to process Nanopore and Illumina reads, assemble BFG genomes, polish those genomes, and correct out-of-frame ORFs for MLST alignment. This also includes GenBank reference genomes referred to in the manuscript. tree_files/ includes pylogenetic tree files and aligned sequence files used in Figures 1, 5, and 6 genomes/ contains different versions of BFG genomes with and without different types of polishing and frame-correction: genomes/pacbio_uncorrected/ contains genomes sequenced with PacBio and assembled with PacBio software Analyzed for MLST trees in Figures: 1, 5, 6 Analyzed in Figures: 5, 6, S7, S9 - S16 genomes/nanopore_racon_medaka/ contains genomes sequenced with Nanopore, assembled with Flye, then polished with racon and medaka. Analyzed in Figures: 5, 6, S7, S9 - S16 genomes/nanopore_racon_medaka_pilon/ contains genomes sequenced with Nanopore, assembled with Flye, polished with racon and medaka, then polished with Illumina reads with pilon. Analyzed in Figures: 5, 6, S7, S9 - S16 genomes/proovframe_BFG_genomes/ contains genomes from the pacbio_uncorrected/, nanopore_racon_medaka/, and nanopore_racon_medaka_pilon/ directories that were frame-corrected with Proovframe. Analyzed for Figures: 2, 3, 4, S2, S3, S4, S5, S6, S8 genomes/nanopore_MEGAN_corrected/ contains genomes from the nanopore_racon_medaka/ and nanopore_racon_medaka_pilon/ directories that were frame-corrected with MEGAN Analyzed for MLST trees in Figures: 1, 5, 6