At the EPN campus level structural biology work is carried out in all the 4 institutes (IBS, ESRF, ILL and EMBL). Today all structural biology groups are turningto cryo-EM. The EM demand is therefore extremely high and present in the 4 Institutes. However there are also some other Research Institutes in Grenoble and we are now proposing that the BAG is extended to them (Cell & Plant Physiology Laboratory LPCV, Grenoble Institut des Neurosciences - GIN, Institute for Advanced Biosciences - IAB)In a nutshell, there are around 40 PIs that could benefit from Krios time on the EPN campus (more than 100 different samples). 2 Glacios are available on the EPN campus for pre-screening. Whereas some real structural work could be carried out on Glacios microscopes, for difficult samples the use of a krios is indispensable (heterogeneous sample or limited concentration requiring a large number of images; small protein etc). Only these samples will be selected for Krios time at the BAG level.

At the EPN campus level structural biology work is carried out in all the 4 institutes (IBS, ESRF, ILL and EMBL). Today all structural biology groups are turningto cryo-EM. The EM demand is therefore extremely high and present in the 4 Institutes. However there are also some other Research Institutes in Grenoble and we are now proposing that the BAG is extended to them (Cell & Plant Physiology Laboratory LPCV, Grenoble Institut des Neurosciences - GIN, Institute for Advanced Biosciences - IAB)In a nutshell, there are around 40 PIs that could benefit from Krios time on the EPN campus (more than 100 different samples). 2 Glacios are available on the EPN campus for pre-screening. Whereas some real structural work could be carried out on Glacios microscopes, for difficult samples the use of a krios is indispensable (heterogeneous sample or limited concentration requiring a large number of images; small protein etc). Only these samples will be selected for Krios time at the BAG level.

At the EPN campus level structural biology work is carried out in all the 4 institutes (IBS, ESRF, ILL and EMBL). Today all structural biology groups are turningto cryo-EM. The EM demand is therefore extremely high and present in the 4 Institutes. However there are also some other Research Institutes in Grenoble and we are now proposing that the BAG is extended to them (Cell & Plant Physiology Laboratory LPCV, Grenoble Institut des Neurosciences - GIN, Institute for Advanced Biosciences - IAB)In a nutshell, there are around 40 PIs that could benefit from Krios time on the EPN campus (more than 100 different samples). 2 Glacios are available on the EPN campus for pre-screening. Whereas some real structural work could be carried out on Glacios microscopes, for difficult samples the use of a krios is indispensable (heterogeneous sample or limited concentration requiring a large number of images; small protein etc). Only these samples will be selected for Krios time at the BAG level.

ESRF is privileged to host two state-of-the-art microscopes. Our BAG unites the majority of local labs that utilise cryo-EM to address their diverse structural biology questions, including researchers from the EPN campus (IBS, ESRF, ILL and EMBL) and its vicinity (Cell & Plant Physiology Laboratory LPCV, Grenoble Institut des Neurosciences - GIN, Institute for Advanced Biosciences - IAB).In a nutshell, approximately 30 principal investigators would greatly benefit from Krios time, with 100 different samples proposed. The high demand for EM beamtime is partially met by two Glacios microscopes available on the campus, currently charged with the pre-screening, but also frequently yielding satisfactory structural insights. Nonetheless, the use of a Krios is indispensable for challenging samples (with heterogeneity or limited concentration requiring a large number of images, small proteins, etc.). Only such “difficult cases” will be selected for Krios time at the BAG level.

ESRF is privileged to host two state-of-the-art microscopes. Our BAG unites the majority of local labs that utilise cryo-EM to address their diverse structural biology questions, including researchers from the EPN campus (IBS, ESRF, ILL and EMBL) and its vicinity (Cell & Plant Physiology Laboratory LPCV, Grenoble Institut des Neurosciences - GIN, Institute for Advanced Biosciences - IAB).In a nutshell, approximately 30 principal investigators would greatly benefit from Krios time, with 100 different samples proposed. The high demand for EM beamtime is partially met by two Glacios microscopes available on the campus, currently charged with the pre-screening, but also frequently yielding satisfactory structural insights. Nonetheless, the use of a Krios is indispensable for challenging samples (with heterogeneity or limited concentration requiring a large number of images, small proteins, etc.). Only such “difficult cases” will be selected for Krios time at the BAG level.

ESRF is privileged to host two state-of-the-art microscopes. Our BAG unites the majority of local labs that utilise cryo-EM to address their diverse structural biology questions, including researchers from the EPN campus (IBS, ESRF, ILL and EMBL) and its vicinity (Cell & Plant Physiology Laboratory LPCV, Grenoble Institut des Neurosciences - GIN, Institute for Advanced Biosciences - IAB).In a nutshell, approximately 30 principal investigators would greatly benefit from Krios time, with 100 different samples proposed. The high demand for EM beamtime is partially met by two Glacios microscopes available on the campus, currently charged with the pre-screening, but also frequently yielding satisfactory structural insights. Nonetheless, the use of a Krios is indispensable for challenging samples (with heterogeneity or limited concentration requiring a large number of images, small proteins, etc.). Only such “difficult cases” will be selected for Krios time at the BAG level.

ESRF is privileged to host two state-of-the-art microscopes. Our BAG unites the majority of local labs that utilise cryo-EM to address their diverse structural biology questions, including researchers from the EPN campus (IBS, ESRF, ILL and EMBL) and its vicinity (Cell & Plant Physiology Laboratory LPCV, Grenoble Institut des Neurosciences - GIN, Institute for Advanced Biosciences - IAB).In a nutshell, approximately 30 principal investigators would greatly benefit from Krios time, with 100 different samples proposed. The high demand for EM beamtime is partially met by two Glacios microscopes available on the campus, currently charged with the pre-screening, but also frequently yielding satisfactory structural insights. Nonetheless, the use of a Krios is indispensable for challenging samples (with heterogeneity or limited concentration requiring a large number of images, small proteins, etc.). Only such “difficult cases” will be selected for Krios time at the BAG level.

ESRF is privileged to host two state-of-the-art microscopes. Our BAG unites the majority of local labs that utilise cryo-EM to address their diverse structural biology questions, including researchers from the EPN campus (IBS, ESRF, ILL and EMBL) and its vicinity (Cell & Plant Physiology Laboratory LPCV, Grenoble Institut des Neurosciences - GIN, Institute for Advanced Biosciences - IAB).In a nutshell, approximately 30 principal investigators would greatly benefit from Krios time, with 100 different samples proposed. The high demand for EM beamtime is partially met by two Glacios microscopes available on the campus, currently charged with the pre-screening, but also frequently yielding satisfactory structural insights. Nonetheless, the use of a Krios is indispensable for challenging samples (with heterogeneity or limited concentration requiring a large number of images, small proteins, etc.). Only such “difficult cases” will be selected for Krios time at the BAG level.

ESRF is privileged to host two state-of-the-art microscopes. Our BAG unites the majority of local labs that utilise cryo-EM to address their diverse structural biology questions, including researchers from the EPN campus (IBS, ESRF, ILL and EMBL) and its vicinity (Cell & Plant Physiology Laboratory LPCV, Grenoble Institut des Neurosciences - GIN, Institute for Advanced Biosciences - IAB).In a nutshell, approximately 30 principal investigators would greatly benefit from Krios time, with 100 different samples proposed. The high demand for EM beamtime is partially met by two Glacios microscopes available on the campus, currently charged with the pre-screening, but also frequently yielding satisfactory structural insights. Nonetheless, the use of a Krios is indispensable for challenging samples (with heterogeneity or limited concentration requiring a large number of images, small proteins, etc.). Only such “difficult cases” will be selected for Krios time at the BAG level.

The work is related to studying the evolution of the allosteric specificity for AMP in the last common ancestor of Pyruvate Kinase (PK) enzymes from methanogenic archaea. This, since there is no X-ray structure of PK from methanogenic archaea, and to obtain structural information of allosteric regulation of this protein can help to understand the glycolytic pathway flux control in methanogenic archaea. We currently have screened cryo-EM grids for this protein obtaining good quality 2D classes from different orientations of the protein. Now we require to collect data of a more concentrated sample, to obtain more particles in the less favored orientation.