Raw data for the article "Design of a microfabrication pipeline combining PDMS soft lithography and vat photopolimerization for the engineering of various microfluidic systems to study tissues and multicellular organisms" by Meynard T., Royer F., Houssier R. et al. (2025).

Raw data for the article "Design of a microfabrication pipeline combining PDMS soft lithography and vat photopolimerization for the engineering of various microfluidic systems to study tissues and multicellular organisms" by Meynard T., Royer F., Houssier R. et al. (2025).

Neutrophil subsets endowed with regulatory/suppressive properties are widely regarded as deleterious immune cells that can jeopardize antitumoral response and/or antimicrobial resistance. Here, we describe a sizeable fraction of neutrophils characterized by the expression of Programmed death-ligand 1 (PD-L1) in biological fluids of humans and mice with severe viral respiratory infections (VRI). Biological and transcriptomic approaches indicated that VRI-driven PD-L1+ neutrophils are endowed with potent regulatory functions and reduced classical antimicrobial properties, as compared to their PD-L1- counterparts. VRI-induced regulatory PD-L1+ neutrophils were generated remotely in the bone marrow in an IFN-γ-dependent manner and were quickly mobilized into the inflamed lungs where they fulfilled their maturation. Neutrophil depletion and PD-L1 blockade during experimental VRI resulted in higher mortality, increased local inflammation, and reduced expression of resolving factors. These findings suggest that PD-L1+ neutrophils are important players in disease tolerance by mitigating local inflammation during severe VRI and that they may constitute relevant targets for future immune interventions.

Supplementary material Contains the Mathematica notebook for analytical and numerical computations, and figure generation.An Rscript used to reproduce the coevolutionary figures from section "Coevolution"The set of appendices in a .pdf file.

Supplementary material Contains the Mathematica notebook for analytical and numerical computations, and figure generation.An Rscript used to reproduce the coevolutionary figures from section "Coevolution"The set of appendices in a .pdf file.

Supplementary material Contains the Mathematica notebook for analytical and numerical computations, and figure generation.An Rscript used to reproduce the coevolutionary figures from section "Coevolution"The set of appendices in a .pdf file.

Schistosomes are gonochoric blood parasites with a complex life cycle responsible for a disease of considerable medical and veterinary importance in tropical and subtropical regions. Understanding the evolution of schistosome genetic diversity is clearly of fundamental importance to interpreting schistosomiasis epidemiology and disease transmission patterns of this parasite. In this paper we have the putative role of the host immune system in the selection of male genetic diversity. We demonstrated the link between genetic dissimilarity and the protective effect among male worms. We then compared the proteomes of three male clones with different genotypes and differing by their capacity to protect against reinfection. The identified differences correspond mainly to antigens known or supposed to be involved in the induction of protective immunity. These results underline the role played by host immune system in the selection of schistosome genetic diversity that is linked to antigenic diversity. We discuss the evolutionary consequences in the context of schistosome infection.