Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.Fluorescence microscopy of live yeast cells at mid-exponential growth. All strains produce the aggregate marker protein gus1-3-GFP or the metacaspase Mca1 tagged with GFP (Mca1-GFP)The dataset was collected through fluorescence microscopy.The image files are provided in Carl Zeiss Image format (.czi).

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.Fluorescence microscopy of fixated yeast cells at mid-exponential growth. The samples have been stained with antibodies against Hsp42 (primary antibody: rabbit anti Hsp42, secondary antibody: goat anti rabbit Alexa Flour488) and counterstained with DAPI (datasets from 170505 and 170608) and antibodies against Actin (primary antibody: mouse anti- Act1, secondary antibody: goat anti mouse Alexa Flour 568) (dataset from 170518) or nucleoporin complexes (dataset from 170608) (primary antibody: mouse anti- Nsp1, secondary antibody: goat anti mouse Alexa Flour 568).The dataset was collected through fluorescence microscopy.The image files are provided in Carl Zeiss Image format (.czi).

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.Fluorescence microscopy of live yeast cells at mid-exponential growth. All strains produce the aggregate marker protein gus1-3-GFP or the metacaspase Mca1 tagged with GFP (Mca1-GFP)The dataset was collected through fluorescence microscopy.The image files are provided in Carl Zeiss Image format (.czi).

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.Fluorescence microscopy of live yeast cells at mid-exponential growth and after 30 minutes of heat shock. The cells were grown in complete synthetic media lacking histidine (CSM-HIS) with 2 % galactose as carbon source. All strains carry a plasmid expressing GFP-HSP104 under the control of the GAL-promoter.The dataset was collected through fluorescence microscopy.The image files are provided in Carl Zeiss Image format (.czi).

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.WT ssa12DD and ssa12DDSSA4OE: Western blots of SDS-PAGE gels with protein extracts from yeast in mid-exponential growth. The yeast was grown in complete synthetic media with 2 % glucose as carbon source. WT ssa12DD w chimaera plasmids: Western blots of SDS-PAGE gels with protein extracts from yeast in mid-exponential growth before and after 30 minutes of heat shock. The yeast was grown in complete synthetic media lacking leucine with 2 % glucose as carbon source. The images are scans of western blot PVDF-membranes.The image files are provided in TIFF format.

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.Fluorescence microscopy of live yeast cells at mid-exponential growth before and after 30 minutes of heat shock. All strains produce the Hsp70 chaperone protein Ssa4 tagged with GFP (Ssa4-GFP) and the metacaspase Mca1 tagged with RFP (Mca1-RFP).The dataset was collected through fluorescence microscopy.The image files are provided in Carl Zeiss Image format (.czi).

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.Fluorescence microscopy of live yeast cells at mid-exponential growth. The yeast is grown in complete synthetic medium with 2 % glucose as carbon source. All strains produce the aggregate marker protein guk1-7-GFP.The dataset was collected through fluorescence microscopy.The image files are provided in Carl Zeiss Image format (.czi).

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.Fluorescence microscopy of live yeast cells at mid-exponential growth before stress, directly after 30 minutes of heat shock and 40 minutes after heat shock. The yeast is grown in complete synthetic medium lacking uracil with 2 % glucose as carbon source. All strains produce the aggregate marker protein guk1-7-GFP and carry a plasmid expressing SIK1-RFP under the control of the GPD-promoter.The dataset was collected through fluorescence microscopy.The image files are provided in Carl Zeiss Image format (.czi).

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.Fluorescence microscopy of live yeast cells at mid-exponential growth and after 30 minutes of heat shock. The cells were grown in complete synthetic media lacking histidine and leucine with 2 % galactose as carbon source. All strains carry two plasmids; one expressing GFP-HSP104 under the control of the GAL-promoter and one expressing SSA1, SSA4 or SSA1/SSA4 chimaeras (alternatively an empty plasmid) under the control of the GPD-promoter.The dataset was collected through fluorescence microscopy.The image files are provided in Carl Zeiss Image format (.czi).

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.Timelapse microscopy of yeast cells during recovery after 30 minutes of heat shock. The yeast was cultivated in complete synthetic media with 2 % glucose as carbon source. All strains produce the misfolding protein guk1-7-GFP.The dataset was collected through timelapse microscopy of the same yeast cells for 2 hours.The image files are provided in Carl Zeiss Image format (.czi).