These datasets are the averaged reconstructions of cytokinesis in six steps that were produced by applying Zach Marin's 'Cytokinesis Pseudotime Analysis' package (https://github.com/zacsimile/cytokinesis-pseudotime-analysis) on a collection of 1930 fluorescence spinning disk confocal images. The data are fluorescence images of expanded NRK49F cells, undergoing cytokinesis. The images were acquired as z-stacks with 1 µm spacing and a X/Y-pixel size of 90 nm on a spinning disk confocal microscope.Applying an averaged expansion factor of 4.444, this results in an effective X/Y pixel size of 20.25 nm and 225 nm in Z-direction. These properties can be adjusted for the correct representation of the averaged reconstructions when opening the files in FIJI/ImageJ.The files are composed of 6 time points, representing 6 differet stages of cytokinesis (RC, CS, RS, SM, BA, A).The files are referenced as Supplementary Files 1-4 in the associated preprint. The table below summarizes the file details.z-stack is a volumetric averagemean-proj is a 2D mean projected averageradial-proj is the axio-radial distribution along the main axis of the cytokinetic bridgeFileFile nameDescriptionSupplementary File 1pseudotime_images_z-stack_reduced_MTs_sen_DAI_Cek_MK1_Ra1_PR1_CiK_ann_myA_myB_acn_Se7_Se1_Se9_BO4_Tsm_ALm_IS1_CHB_16bit.tif5D file including all targets that were analyzed and averaged in this study in the 6 cytokinetic stages (order: RC, CS, RS, SM, BA, A)Supplementary File 2pseudotime_images_mean-proj_MTs_sen_DAI_Cek_MK1_Ra1_PR1_CiK_ann_myA_myB_acn_Se7_Se1_Se9_BO4_Tsm_ALm_IS1_CHB_16bit.tif4D file, mean projections of all targets that were analyzed and averaged in this study in the 6 cytokinetic stages (order: RC, CS, RS, SM, BA, A)Supplementary File 3pseudotime_images_radial-proj_MTs_sen_DAI_Cek_MK1_Ra1_PR1_CiK_ann_myA_myB_acn_Se7_Se1_Se9_BO4_Tsm_ALm_IS1_CHB_16bit.tif4D file, axio-radial projections of all targets that were analyzed and averaged in this study in the 6 cytokinetic stages (order: RC, CS, RS, SM, BA, A)Supplementary File 4pseudotime_images_mean-proj_MTs_sen_DAI_IS1_CHB_16bit.tif4D file, mean projections of the sub-stages of ESCRT-III cone assembly (order: RC, CS, RS, SM0, SM1, SM2, SM3, BA0, BA1, BA2, BA3, BA4, A)The order of the channels in Suppl. Files 1-3 is as followsMTs       tubulinsen         Septin2-GFPDAI        DAPICek        CellMask orangeMK1      MKLP1Ra1        MgcRacGAP1PR1        PRC1CiK         Citron Kinaseann        AnillinmyA      Myosin IIAmyB       Myosin IIBacn         actinSe7        Septin7 Se1        Septin11Se9        Septin9BO4       BORG4Tsm       Tsg101ALm       ALIXIS1          IST1CHB       CHMP4B

These datasets are the averaged reconstructions of cytokinesis in six steps that were produced by applying Zach Marin's 'Cytokinesis Pseudotime Analysis' package (https://github.com/zacsimile/cytokinesis-pseudotime-analysis) on a collection of 1930 fluorescence spinning disk confocal images. The data are fluorescence images of expanded NRK49F cells, undergoing cytokinesis. The images were acquired as z-stacks with 1 µm spacing and a X/Y-pixel size of 90 nm on a spinning disk confocal microscope.Applying an averaged expansion factor of 4.444, this results in an effective X/Y pixel size of 20.25 nm and 225 nm in Z-direction. These properties can be adjusted for the correct representation of the averaged reconstructions when opening the files in FIJI/ImageJ.The files are composed of 6 time points, representing 6 differet stages of cytokinesis (RC, CS, RS, SM, BA, A).The files are referenced as Supplementary Files 1-4 in the associated preprint. The table below summarizes the file details.z-stack is a volumetric averagemean-proj is a 2D mean projected averageradial-proj is the axio-radial distribution along the main axis of the cytokinetic bridgeFileFile nameDescriptionSupplementary File 1pseudotime_images_z-stack_reduced_MTs_sen_DAI_Cek_MK1_Ra1_PR1_CiK_ann_myA_myB_acn_Se7_Se1_Se9_BO4_Tsm_ALm_IS1_CHB_16bit.tif5D file including all targets that were analyzed and averaged in this study in the 6 cytokinetic stages (order: RC, CS, RS, SM, BA, A)Supplementary File 2pseudotime_images_mean-proj_MTs_sen_DAI_Cek_MK1_Ra1_PR1_CiK_ann_myA_myB_acn_Se7_Se1_Se9_BO4_Tsm_ALm_IS1_CHB_16bit.tif4D file, mean projections of all targets that were analyzed and averaged in this study in the 6 cytokinetic stages (order: RC, CS, RS, SM, BA, A)Supplementary File 3pseudotime_images_radial-proj_MTs_sen_DAI_Cek_MK1_Ra1_PR1_CiK_ann_myA_myB_acn_Se7_Se1_Se9_BO4_Tsm_ALm_IS1_CHB_16bit.tif4D file, axio-radial projections of all targets that were analyzed and averaged in this study in the 6 cytokinetic stages (order: RC, CS, RS, SM, BA, A)Supplementary File 4pseudotime_images_mean-proj_MTs_sen_DAI_IS1_CHB_16bit.tif4D file, mean projections of the sub-stages of ESCRT-III cone assembly (order: RC, CS, RS, SM0, SM1, SM2, SM3, BA0, BA1, BA2, BA3, BA4, A)The order of the channels in Suppl. Files 1-3 is as followsMTs       tubulinsen         Septin2-GFPDAI        DAPICek        CellMask orangeMK1      MKLP1Ra1        MgcRacGAP1PR1        PRC1CiK         Citron Kinaseann        AnillinmyA      Myosin IIAmyB       Myosin IIBacn         actinSe7        Septin7 Se1        Septin11Se9        Septin9BO4       BORG4Tsm       Tsg101ALm       ALIXIS1          IST1CHB       CHMP4B

This archive contains raw Single Particle Tracking (SPT) data as exported from MINFLUX-iMSCPECTOR (commercial version - 16.3.15645-m2205) in the .npy file-format as well as SPT image stacks taken on a custom TIRF Microscope with custom control software. Information on the experimental conditions and sample generation are found within the related preprint The MINFLUX sequence files used to control the device are located in the related GitHub repository.Related preprint.Related GitHub repository.(Working title: "Parameter Optimization for MINFLUX Microscopy enabled Single Particle Tracking.")

This archive contains raw Single Particle Tracking (SPT) data as exported from MINFLUX-iMSCPECTOR (commercial version - 16.3.15645-m2205) in the .npy file-format as well as SPT image stacks taken on a custom TIRF Microscope with custom control software. Information on the experimental conditions and sample generation are found within the related preprint The MINFLUX sequence files used to control the device are located in the related GitHub repository.Related preprint.Related GitHub repository.(Working title: "Parameter Optimization for MINFLUX Microscopy enabled Single Particle Tracking.")

This file contains the source data used in Henggeler et al., organized by figure and figure panel for clarity.

This file contains the source data used in Henggeler et al., organized by figure and figure panel for clarity.

These datasets represent raw data for the preparation of Figures in:Ferraioli S, Sarigol F, Prakash C, Filipczak D, Foisner R, Naetar N. (2024) LAP2alpha facilitates myogenic gene expression by preventing nucleoplasmic lamin A/C from spreading to active chromatin regions. Nucleic Acids Res.2024 Sep 4:gkae752. doi: 10.1093/nar/gkae752.

These datasets represent raw data for the preparation of Figures in:Ferraioli S, Sarigol F, Prakash C, Filipczak D, Foisner R, Naetar N. (2024) LAP2alpha facilitates myogenic gene expression by preventing nucleoplasmic lamin A/C from spreading to active chromatin regions. Nucleic Acids Res.2024 Sep 4:gkae752. doi: 10.1093/nar/gkae752.

Mitochondria are fundamental for life and require balanced ion exchange to maintain proper functioning. The mitochondrial cation exchanger LETM1 sparks interest because of its pathophysiological role in seizures in the Wolf Hirschhorn Syndrome (WHS). Despite observation of sleep disorganization in epileptic WHS patients, and growing studies linking mitochondria and epilepsy to circadian rhythms, LETM1 has not been studied from the chronobiological perspective. Here we established a viable letm1 knock-out, using the diurnal vertebrate Danio rerio to study the metabolic and chronobiological consequences of letm1 deficiency. We report diurnal rhythms of Letm1 protein levels in wild-type fish. We show that mitochondrial nucleotide metabolism is deregulated in letm1-/- mutant fish, the rate limiting enzyme of NAD+ production is up-regulated, while NAD+ and NADH pools are reduced. These changes were associated with increased expression amplitude of circadian core clock genes in letm1-/- compared to wild-type under light/dark conditions, suggesting decreased NAD(H) levels as a possible mechanism for circadian system perturbation in Letm1 deficiency. Replenishing NAD pool may ameliorate WHS-associated sleep and neurological disorders.

Many species synchronize their physiology and behavior to specific hours. It is commonly assumed that sunlight acts as the main entrainment signal for ~24h clocks. However, the moon provides similarly regular time information. Consistently, a growing number of studies have reported correlations between diel behavior and lunidian cycles. Yet, mechanistic insight into the possible influences of the moon on ~24hr timers remains scarce. We have explored the marine bristleworm Platynereis dumerilii to investigate the role of moonlight in the timing of daily behavior. We uncover that moonlight, besides its role in monthly timing, also schedules the exact hour of nocturnal swarming onset to the nights’ darkest times. Our work reveals that extended moonlight impacts on a plastic clock that exhibits <24h (moonlit) or >24h (no moon) periodicity. Abundance, light sensitivity, and genetic requirement indicate that the Platynereis light receptor molecule r-Opsin1 serves as a receptor that senses moonrise, whereas the cryptochrome protein L-Cry is required to discriminate the proper valence of nocturnal light as either moon- or sunlight. Comparative experiments in Drosophila suggest that cryptochrome’s principal requirement for light valence interpretation is conserved. Its exact biochemical properties differ, however, between species with dissimilar timing ecology. Our work advances the molecular understanding of lunar impact on fundamental rhythmic processes, including those of marine mass spawners endangered by anthropogenic change.