Automated Author Profile

Shields, Mallory

Current S-Index

61.1

Sum of Dataset Indices for all datasets

Average Dataset Index per Dataset

1.9

Average Dataset Index per dataset

Total Datasets

33

Total datasets for this author

Average FAIR Score

76.1%

Average FAIR Score per dataset

Total Citations

0

Total citations to the author's datasets

Total Mentions

0

Total mentions of the author's datasets

S-Index Interpretation

S-Index Over Time

Cumulative Citations Over Time

Cumulative Mentions Over Time

Datasets

Dataset associated with "The role of the C2A domain of synaptotagmin 1 in asynchronous neurotransmitter release"

Following nerve stimulation, there are two distinct phases of Ca2+-dependent neurotransmitter release: a fast, synchronous release phase, and a prolonged, asynchronous release phase. Each of these phases is tightly regulated and mediated by distinct mechanisms. Synaptotagmin 1 is the major Ca2+ sensor that triggers fast, synchronous neurotransmitter release upon Ca2+ binding by its C2A and C2B domains. It has also been implicated in the inhibition of asynchronous neurotransmitter release, as blocking Ca2+ binding by the C2A domain of synaptotagmin 1 results in increased asynchronous release. However, the mutation used to block Ca2+ binding in the previous experiments (aspartate to asparagine mutations, sytD-N) had the unintended side effect of mimicking Ca2+ binding, raising the possibility that the increase in asynchronous release was directly caused by ostensibly constitutive Ca2+ binding. Thus, rather than modulating an asynchronous sensor, sytD-N may be mimicking one. To directly test this C2A inhibition hypothesis, we utilized an alternate C2A mutation that we designed to block Ca2+ binding without mimicking it (an aspartate to glutamate mutation, sytD-E). Analysis of both the original sytD-N mutation and our alternate sytD-E mutation at the Drosophila neuromuscular junction showed differential effects on asynchronous release, as well as on synchronous release and the frequency of spontaneous release. Importantly, we found that asynchronous release is not increased in the sytD-E mutant. Thus, our work provides new mechanistic insight into synaptotagmin 1 function during Ca2+-evoked synaptic transmission and demonstrates that Ca2+ binding by the C2A domain of synaptotagmin 1 does not inhibit asynchronous neurotransmitter release in vivo.

Authors

  • Shields, Mallory ;
  • Bowers, Matthew ;
  • Kramer, Hannah ;
  • Fulcer, McKenzie ;
  • Perinet, Lara ;
  • Metz, Marissa ;
  • Reist, Noreen
0 Citations0 Mentions50% FAIR1.2 Dataset Index
10.25675/10217/2049142020

Fig2C Blot2

No description available

Authors

  • Bowers, Matthew ;
  • Shields, Mallory ;
  • Fulcer, McKenzie ;
  • Bollig, Madelyn ;
  • Rock, Patrick ;
  • Sutton, Roger ;
  • Vrailas-Mortimer, Alysia ;
  • Lochmüller, Hanns ;
  • Whittaker, Roger ;
  • Horvath, Rita ;
  • Reist, Noreen
0 Citations0 Mentions77% FAIR1.9 Dataset Index
10.5061/dryad.st621/22017

Fig2C_Blot1

No description available

Authors

  • Shields, Mallory ;
  • Bowers, Matthew ;
  • Fulcer, McKenzie ;
  • Bollig, Madelyn ;
  • Rock, Patrick ;
  • Sutton, Roger ;
  • Vrailas-Mortimer, Alysia ;
  • Lochmüller, Hanns ;
  • Whittaker, Roger ;
  • Horvath, Rita ;
  • Reist, Noreen
0 Citations0 Mentions77% FAIR1.9 Dataset Index
10.5061/dryad.st621/32017

Fig2B_Blot2

No description available

Authors

  • Shields, Mallory ;
  • Bowers, Matthew ;
  • Fulcer, McKenzie ;
  • Bollig, Madelyn ;
  • Rock, Patrick ;
  • Sutton, Roger ;
  • Vrailas-Mortimer, Alysia ;
  • Lochmüller, Hanns ;
  • Whittaker, Roger ;
  • Horvath, Rita ;
  • Reist, Noreen
0 Citations0 Mentions77% FAIR1.9 Dataset Index
10.5061/dryad.st621/42017

Fig2B_Blot1

No description available

Authors

  • Shields, Mallory ;
  • Bowers, Matthew ;
  • Fulcer, McKenzie ;
  • Bollig, Madelyn ;
  • Rock, Patrick ;
  • Sutton, Roger ;
  • Vrailas-Mortimer, Alysia ;
  • Lochmüller, Hanns ;
  • Whittaker, Roger ;
  • Horvath, Rita ;
  • Reist, Noreen
0 Citations0 Mentions77% FAIR1.9 Dataset Index
10.5061/dryad.st621/52017

Fig2A_Blot2

No description available

Authors

  • Shields, Mallory ;
  • Bowers, Matthew ;
  • Fulcer, McKenzie ;
  • Bollig, Madelyn ;
  • Rock, Patrick ;
  • Sutton, Roger ;
  • Vrailas-Mortimer, Alysia ;
  • Lochmüller, Hanns ;
  • Whittaker, Roger ;
  • Horvath, Rita ;
  • Reist, Noreen
0 Citations0 Mentions77% FAIR1.9 Dataset Index
10.5061/dryad.st621/62017

Fig2A_Blot1

No description available

Authors

  • Shields, Mallory ;
  • Bowers, Matthew ;
  • Fulcer, McKenzie ;
  • Bollig, Madelyn ;
  • Rock, Patrick ;
  • Sutton, Roger ;
  • Vrailas-Mortimer, Alysia ;
  • Lochmüller, Hanns ;
  • Whittaker, Roger ;
  • Horvath, Rita ;
  • Reist, Noreen
0 Citations0 Mentions77% FAIR1.9 Dataset Index
10.5061/dryad.st621/72017

Fig2_2A

No description available

Authors

  • Shields, Mallory ;
  • Bowers, Matthew ;
  • Fulcer, McKenzie ;
  • Bollig, Madelyn ;
  • Rock, Patrick ;
  • Sutton, Roger ;
  • Vrailas-Mortimer, Alysia ;
  • Lochmüller, Hanns ;
  • Whittaker, Roger ;
  • Horvath, Rita ;
  • Reist, Noreen
0 Citations0 Mentions77% FAIR1.9 Dataset Index
10.5061/dryad.st621/82017

Fig2_2B

No description available

Authors

  • Shields, Mallory ;
  • Bowers, Matthew ;
  • Fulcer, McKenzie ;
  • Bollig, Madelyn ;
  • Rock, Patrick ;
  • Sutton, Roger ;
  • Vrailas-Mortimer, Alysia ;
  • Lochmüller, Hanns ;
  • Whittaker, Roger ;
  • Horvath, Rita ;
  • Reist, Noreen
0 Citations0 Mentions77% FAIR1.9 Dataset Index
10.5061/dryad.st621/92017

Fig2_2C

No description available

Authors

  • Shields, Mallory ;
  • Bowers, Matthew ;
  • Fulcer, McKenzie ;
  • Bollig, Madelyn ;
  • Rock, Patrick ;
  • Sutton, Roger ;
  • Vrailas-Mortimer, Alysia ;
  • Lochmüller, Hanns ;
  • Whittaker, Roger ;
  • Horvath, Rita ;
  • Reist, Noreen
0 Citations0 Mentions77% FAIR1.9 Dataset Index
10.5061/dryad.st621/102017