Automated Author Profile

Webb, Shaun

Current S-Index

7.4

Sum of Dataset Indices for all datasets

Average Dataset Index per Dataset

1.1

Average Dataset Index per dataset

Total Datasets

7

Total datasets for this author

Average FAIR Score

48.6%

Average FAIR Score per dataset

Total Citations

3

Total citations to the author's datasets

Total Mentions

0

Total mentions of the author's datasets

S-Index Interpretation

S-Index Over Time

Cumulative Citations Over Time

Cumulative Mentions Over Time

Datasets

Supplementary data for the manuscript "Quantitative modelling predicts the impact of DNA methylation on RNA polymerase II traffic"

Patterns of gene expression are primarily determined by proteins that locally enhance or repress transcription. While many transcription factors target a restricted number of genes, others appear to modulate transcription levels globally. An example is MeCP2, an abundant methylated-DNA binding protein that is mutated in the neurological disorder Rett Syndrome. Despite much research, the molecular mechanism by which MeCP2 regulates gene expression is not fully resolved. Here we integrate quantitative, multi-dimensional experimental analysis and mathematical modelling to show that MeCP2 is a novel type of global transcriptional regulator whose binding to DNA creates "slow sites" in gene bodies. Waves of slowed-down RNA polymerase II formed behind these sites travel backward and indirectly affect initiation, reminiscent of defect-induced shock waves in non-equilibrium physics transport models. This mechanism differs from conventional gene regulation mechanisms, which often involve direct modulation of transcription initiation. Our findings point to a genome-wide function of DNA methylation that may account for the reversibility of Rett syndrome in mice. Moreover, our combined theoretical and experimental approach provides a general method for understanding how global gene expression patterns are choreographed.

Authors

  • Waclaw, Bartlomiej ;
  • Cholewa-Waclaw, Justyna ;
  • Shah, Ruth ;
  • Webb, Shaun ;
  • Chhatbar, Kashyap ;
  • Ramsahoye, Bernard ;
  • Pusch, Oliver ;
  • Yu, Miao ;
  • Greulich, Philip ;
  • Bird, Adrian
1 Citation0 Mentions46% FAIR0.8 Dataset Index
10.7488/ds/2568January 2019

Additional file 3: Table S2. of RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination

Supplementary data showing protein names, SILAC ratios (more than two-fold), peptide counts, and peptide intensities identified by SILAC MS in wild-type and TRIM25 KO HeLa cells. (XLSX 33 kb)

Authors

  • Choudhury, Nila ;
  • Heikel, Gregory ;
  • Trubitsyna, Maryia ;
  • Kubik, Peter ;
  • Nowak, Jakub ;
  • Webb, Shaun ;
  • Granneman, Sander ;
  • Spanos, Christos ;
  • Rappsilber, Juri ;
  • Castello, Alfredo ;
  • Gracjan Michlewski
0 Citations0 Mentions85% FAIR1.8 Dataset Index
10.6084/m9.figshare.c.3925501_d3January 2017

Additional file 4: Table S3. of RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination

Supplementary data showing gene names and number of RNA reads derived from three independent T7-TRIM25 CLIP-seq experiments. (XLSX 117 kb)

Authors

  • Choudhury, Nila ;
  • Heikel, Gregory ;
  • Trubitsyna, Maryia ;
  • Kubik, Peter ;
  • Nowak, Jakub ;
  • Webb, Shaun ;
  • Granneman, Sander ;
  • Spanos, Christos ;
  • Rappsilber, Juri ;
  • Castello, Alfredo ;
  • Gracjan Michlewski
0 Citations0 Mentions85% FAIR1.8 Dataset Index
10.6084/m9.figshare.c.3925501_d4January 2017

Additional file 2: Table S1. of RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination

Supplementary data showing protein names, SILAC ratios, peptide counts, and peptide intensities identified by SILAC MS in T7-TRIM25, T7-TRIM25Î RBD, and T7-TRIM25â +â RNases co-immunoprecipitation assays. (XLSX 22 kb)

Authors

  • Choudhury, Nila ;
  • Heikel, Gregory ;
  • Trubitsyna, Maryia ;
  • Kubik, Peter ;
  • Nowak, Jakub ;
  • Webb, Shaun ;
  • Granneman, Sander ;
  • Spanos, Christos ;
  • Rappsilber, Juri ;
  • Castello, Alfredo ;
  • Gracjan Michlewski
0 Citations0 Mentions85% FAIR1.8 Dataset Index
10.6084/m9.figshare.c.3925501_d2January 2017

Additional file 3: Table S2. of RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination

Supplementary data showing protein names, SILAC ratios (more than two-fold), peptide counts, and peptide intensities identified by SILAC MS in wild-type and TRIM25 KO HeLa cells. (XLSX 33 kb)

Authors

  • Choudhury, Nila ;
  • Heikel, Gregory ;
  • Trubitsyna, Maryia ;
  • Kubik, Peter ;
  • Nowak, Jakub ;
  • Webb, Shaun ;
  • Granneman, Sander ;
  • Spanos, Christos ;
  • Rappsilber, Juri ;
  • Castello, Alfredo ;
  • Gracjan Michlewski
0 Citations0 Mentions13% FAIR0.1 Dataset Index
10.6084/m9.figshare.c.3925501_d3.v1January 2017

Additional file 2: Table S1. of RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination

Supplementary data showing protein names, SILAC ratios, peptide counts, and peptide intensities identified by SILAC MS in T7-TRIM25, T7-TRIM25Î RBD, and T7-TRIM25â +â RNases co-immunoprecipitation assays. (XLSX 22 kb)

Authors

  • Choudhury, Nila ;
  • Heikel, Gregory ;
  • Trubitsyna, Maryia ;
  • Kubik, Peter ;
  • Nowak, Jakub ;
  • Webb, Shaun ;
  • Granneman, Sander ;
  • Spanos, Christos ;
  • Rappsilber, Juri ;
  • Castello, Alfredo ;
  • Gracjan Michlewski
1 Citation0 Mentions13% FAIR0.5 Dataset Index
10.6084/m9.figshare.c.3925501_d2.v1January 2017

Additional file 4: Table S3. of RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination

Supplementary data showing gene names and number of RNA reads derived from three independent T7-TRIM25 CLIP-seq experiments. (XLSX 117 kb)

Authors

  • Choudhury, Nila ;
  • Heikel, Gregory ;
  • Trubitsyna, Maryia ;
  • Kubik, Peter ;
  • Nowak, Jakub ;
  • Webb, Shaun ;
  • Granneman, Sander ;
  • Spanos, Christos ;
  • Rappsilber, Juri ;
  • Castello, Alfredo ;
  • Gracjan Michlewski
1 Citation0 Mentions13% FAIR0.5 Dataset Index
10.6084/m9.figshare.c.3925501_d4.v1January 2017