An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

Supplementary Fig. S1: Phylogenetic tree, genestructure and motif compositions of LEA2genes in upland cotton. The phylogenetic tree was constructed using MEGA 7.0.Exon/intron structures of LEA genesin upland cotton, exons introns and up/down-stream were represented by yellowboxes, black lines and blue boxes, respectively. Protein motif analysisrepresented by different colours, and each motif represented by number; Supplementary Fig. S2: conserved motifsin upland cotton, G. hirsutum LEA2proteins. The conserved motifs were obtained using MEME program. The colorscheme of the logo indicates amino acid types. Polar: green=uncharged;blue=+vely charged; red=-vely charged; Non-polar: violet/purple=aliphatic. Asdescribed by Dure, 2001; Supplementary Fig.S3: Alignment of the LEA2 protein sequences of Upland cotton, Gossypium hirsutum. Amino acid sequenceswere aligned using the ClustalW2 algorithm. Dashes indicate gaps introduced foroptimal alignment. The typical LEA2 sequence elements are bound in boxes indifferent colours: K segment–red box; Y segment–green; S segment–black; Esegment–gray; R segment–purple; D segment–yellow; Supplementary Fig. S4. LEA2 genesdistribution in A and D cotton chromosomes: Chromosomal position of each LEA2 genes was mapped according to the upland cotton genome. The chromosomenumber is indicated at the top of each chromosome; Supplementary Fig. S5: LEA2genes distribution in upland cotton chromosomes. Chromosomal position of each LEA2 genes was mapped according to the upland cotton genome. The chromosomenumber is indicated at the top of each chromosome; Supplementary Table S1: List of primers used for upland cotton, G. hirsutum LEA2 genes expression analysis under drought stress; Supplementary Table S2: Conserveddomain analysis of LEA2s proteins identified from upland cotton using CDD toolfrom NCBI; Supplementary Table S3:Transmembrane domains in LEA2s identified from upland cotton, using TMHMM andSOSUI servers. ExpAA: The expected number of amino acids in transmembranehelices. First60: The expected number of amino acids in transmembrane helicesin the first 60 amino acids of the protein. MP: Membrane protein; TMH: Thenumber of predicted transmembrane helices; SupplementaryTable S4: Subcellular location as determined by TargetP and Pprowlerprediction tools. SP-secretory pathways; C-chloroplast;-any other location; Supplementary Table S5: Geneduplication and determination of the Ks/Ka in paralogous gene pairs; Supplementary Table S6: Cis element analysis of putative LEA 2 promoters relatedto drought stress and SupplementaryTable S7: LEA2 genes and miRNAtargets

Supplementary Fig. S1: Phylogenetic tree, genestructure and motif compositions of LEA2genes in upland cotton. The phylogenetic tree was constructed using MEGA 7.0.Exon/intron structures of LEA genesin upland cotton, exons introns and up/down-stream were represented by yellowboxes, black lines and blue boxes, respectively. Protein motif analysisrepresented by different colours, and each motif represented by number; Supplementary Fig. S2: conserved motifsin upland cotton, G. hirsutum LEA2proteins. The conserved motifs were obtained using MEME program. The colorscheme of the logo indicates amino acid types. Polar: green=uncharged;blue=+vely charged; red=-vely charged; Non-polar: violet/purple=aliphatic. Asdescribed by Dure, 2001; Supplementary Fig.S3: Alignment of the LEA2 protein sequences of Upland cotton, Gossypium hirsutum. Amino acid sequenceswere aligned using the ClustalW2 algorithm. Dashes indicate gaps introduced foroptimal alignment. The typical LEA2 sequence elements are bound in boxes indifferent colours: K segment–red box; Y segment–green; S segment–black; Esegment–gray; R segment–purple; D segment–yellow; Supplementary Fig. S4. LEA2 genesdistribution in A and D cotton chromosomes: Chromosomal position of each LEA2 genes was mapped according to the upland cotton genome. The chromosomenumber is indicated at the top of each chromosome; Supplementary Fig. S5: LEA2genes distribution in upland cotton chromosomes. Chromosomal position of each LEA2 genes was mapped according to the upland cotton genome. The chromosomenumber is indicated at the top of each chromosome; Supplementary Table S1: List of primers used for upland cotton, G. hirsutum LEA2 genes expression analysis under drought stress; Supplementary Table S2: Conserveddomain analysis of LEA2s proteins identified from upland cotton using CDD toolfrom NCBI; Supplementary Table S3:Transmembrane domains in LEA2s identified from upland cotton, using TMHMM andSOSUI servers. ExpAA: The expected number of amino acids in transmembranehelices. First60: The expected number of amino acids in transmembrane helicesin the first 60 amino acids of the protein. MP: Membrane protein; TMH: Thenumber of predicted transmembrane helices; SupplementaryTable S4: Subcellular location as determined by TargetP and Pprowlerprediction tools. SP-secretory pathways; C-chloroplast;-any other location; Supplementary Table S5: Geneduplication and determination of the Ks/Ka in paralogous gene pairs; Supplementary Table S6: Cis element analysis of putative LEA 2 promoters relatedto drought stress and SupplementaryTable S7: LEA2 genes and miRNAtargets