Genomes contain mosaics of discordant evolutionary histories, challenging the accurate inference of the tree of life. While genome-wide data are routinely used for discordance-aware phylogenomic analyses, due to modeling and scalability limitations, the current practice leaves out large chunks of the genomes. As more high-quality genomes become available, we urgently need discordance-aware methods to infer the tree directly from a multiple genome alignment. Here, we introduce CASTER, a site-based method that eliminates the need to predefine recombination-free loci. CASTER is statistically consistent under incomplete lineage sorting and is scalable to hundreds of mammalian whole genomes. We show both in simulations and on real data that CASTER is scalable and accurate and that its per-site scores can reveal interesting patterns of evolution across the genome.

Simulations used in "Estimating the relative proportions of SARS-CoV-2 strains from wastewater samples"

Simulations used in "Estimating the relative proportions of SARS-CoV-2 strains from wastewater samples"

Multiple Sequence Alignment of imputed SARS-CoV-2 sequences used in 'Estimating the relative proportions of SARS-CoV-2 strains from wastewater samples'

Multiple Sequence Alignment of imputed SARS-CoV-2 sequences used in 'Estimating the relative proportions of SARS-CoV-2 strains from wastewater samples'

Data underlying AncestralClust manuscript

Data underlying AncestralClust manuscript

The giant squid (Architeuthis dux; Steenstrup, 1857) is an enigmatic giant mollusk with a circumglobal distribution in the deep ocean, except in the high Arctic and Antarctic waters. The elusiveness of the species makes it difficult to study. Thus, having a genome assembled for this deep-sea dwelling species will allow unlocking several pending evolutionary questions. We present a draft genome assembly that includes 200 Gb of Illumina reads, 4 Gb of Moleculo synthetic long-reads and 108 Gb of Chicago libraries, with a final size matching the estimated genome size of 2.7 Gb, and a scaffold N50 of 4.8 Mb. We also present an alternative assembly including 27 Gb raw reads generated using the Pacific Biosciences platform. In addition, we sequenced the proteome of the same individual and RNA from three different tissue types from three other species of squid species (Onychoteuthis banksii, Dosidicus gigas, and Sthenoteuthis oualaniensis) to assist genome annotation. We annotated 33,406 protein coding genes supported by evidence and the genome completeness estimated by BUSCO reached 92%. Repetitive regions cover 49.17% of the genome. This annotated draft genome of A. dux provides a critical resource to investigate the unique traits of this species, including its gigantism and key adaptations to deep-sea environments.

The Eastern rockhopper penguin is a small crested penguin. It is closely related to the Western rockhopper penguin, however there is still some debate about whether both are considered incipient species, sub-species or distinct species. Recent genetic work suggests they are probably separate species. The Eastern rockhopper penguin breeds widely on subantarctic islands from New Zealand Campbell/Auckland/Antipodes Islands to the Prince Edward Islands group. The International Union for Conservation of Nature (IUCN) classifies the Southern rockhopper penguin group (including both the Eastern and the Western rockhopper penguin) as vulnerable.
We sequenced the genome of an adult Eastern rockhopper penguin from Possession Island, Crozet Islands (provided by Yves Cherel) to a depth of approximately 163x with short reads from a series of libraries with various insert sizes (250bp-20Kb). The assembled scaffolds of high quality sequences total 1.25Gb, with the contig and scaffold N50 values of 72.54Kb and 6.13Mb respectively. We identified 15963 protein-coding genes.

The Northern rockhopper penguin (also known as Moseley's rockhopper penguin or Moseley's penguin) is a small crested penguin closely related to the Eastern and Western rockhopper penguins. The Northern rockhopper penguin breeds on Amsterdam and Gough islands. They breed in colonies at sea level, on cliff sides and sometimes inland. Populations of the Northern rockhopper penguin have declined drastically, and the International Union for Conservation of Nature (IUCN) classifies them as endangered.
We sequenced the genome of an adult Northern rockhopper penguin from Amsterdam Island, Southern Indian Ocean (provided by Yves Cherel) to a depth of approximately 139x with short reads from a series of libraries with various insert sizes (250bp-20Kb). The assembled scaffolds of high quality sequences total 1.23Gb, with the contig and scaffold N50 values of 20.86Kb and 2.14Mb respectively. We identified 16812 protein-coding genes.