Nanorana parkeri (also known as the high Himalaya frog, Xizang Plateau frog, Parker's slow frog, or mountain slow frog) is a common frog living across the Tibetan Plateau. It occurs at elevations ranging from 2,850 to 5,000m. Because this species lives at such high elevations, it provides an additional excellent biological model to study the frogs adaptations to extreme conditions.
A female frog was collected from the Qinghai-Tibetan Plateau at an elevation of 4,900m, and genomic DNA was extracted from muscle tissue. Paired end DNA libraries with different insert-size lengths (170 bp to 20 kb) weresequenced on the Illumina HiSeq 2000 platform. After performing filtering steps to remove artificial duplication, adaptercontamination and low-quality reads, 190 Gbp of high-quality data (83× genome coverage) was obtained. This was assembled using SOAPdenovo and SSPACE, producing a final draft assembly of 2.0Gb with an N50 scaffold size of 1.05Mb. More than 20,000 genes were predicted. The Nanorana parkeri genome should help offer new insights into the amphibian evolution and Tibetan high-altitude adaptation.

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The evolutionary transition from a lizard-like body to a snake-like body form is oneof the most important transformations in reptilian evolution. The increasing number of reptiliangenomes allows a deeper understanding of vertebrate evolution, although the genetic basisunderlying the loss of limbs in reptiles remains enigmatic. Here we report the sequencing,assembly, and annotation of the genome of Asian glass lizard Ophisaurus gracilis, which is alimbless lizard species with an elongated snake-like body form.
The sequencing of the O. gracilis genome using the Illumina HiSeq2000 platformresulted in a total of 274.20 Gbp raw data, which was subsequently filtered and assembled to afinal assembly of 1.78 Gbp, comprising 6,717 scaffolds with N50=1.27 Mbp. Based on the k-merestimated genome size of 1.71 Gbp, the assembly appears to be near 100% complete. A total of19,513 protein coding genes were predicted, and 884.06 Mbp repeat sequences (approximatelyhalf of the genome) were annotated. The draft genome of O. gracilis shows similar characteristicsto both lizards and snakes, and represents a substantial addition to existing reptilian genomesavailable for comparative analysis.

The domestic goat is one of the most important livestock species in the world, especially in China, India and other developing countries. Goats not only serve as an important source of meat, milk, fiber and pelts, and have fulfilled agricultural, economic, cultural and even religious roles from very early times in human civilization, but also are now used as animal models for biomedical research and transgene production of protein medicines.We would like to share all the genome data of goat. We hope the genome sequence of goat can provide a new resource for biological research and breeding of goat and other small ruminants.
We sequenced the 2.92 Gb genome to a depth of approximately 65.6 X with short reads from a series of libraries with various insert sizes ( 170 bp, 350 bp, 800 bp, 2 kb, 5 kb, 10 kb and 20 kb) on a HiSeq 2000 sequencer.
The assembled scaffolds of high quality sequences total 191.5 Gb, with the contig and scaffold N50 values of 18.7 kb and 2.21 Mb respectively. We identified 22,175 protein-coding genes.In addition, we also provide the restriction-enzyme fragment maps derived from the whole genome mapping (WGM) technology developed by the Argus System (method described in this paper).
Scaffolds derived from de novo assembly of next-generation sequencing data are converted into restriction maps by in silico restriction enzyme digestion. Then, the distance between restriction enzyme sites in the sequencing-derived scaffolds are matched to the lengths of the optical fragments in the single-molecule WGM restriction maps. Matches allow the scaffolds to be extended and linked into super-scaffolds.

Delimiting species and clarifying phylogenetic relationships are the main goals of systematics. For species with questionable taxonomic status, species delimitation approaches using multi-species coalescent models with multiple loci are recommended if morphological data are unavailable or unhelpful. Moreover, these methods will also reduce subjectivity based on genetic distance or requirement of monophyletic genetic lineages. We determine the validity and phylogenetic position of a rare and long controversial species of Chinese reptile, the Szechwan ratsnake (Euprepiophis perlacea), using multi-locus data from multiple individuals and coalescent-based approaches. Species were first delimited using Bayesian Phylogenetics & Phylogeography (BP&P), Brownie and Bayes Factor model comparison approaches, while relationships among species were estimated using species tree inference in *BEAST. Results indicate that Euprepiophis perlacea is a distinct species sister to Euprepiophis mandarinus. Despite gene tree discrepancy, the coalescent model-based approaches used here demonstrate the taxonomic validity and the phylogenetic position of Euprepiophis perlacea. These approaches objectively test the validity of questionable species diagnoses based on morphological characters and determine their phylogenetic position.

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