This dataset contains measurements of the physical phenotype of single cells dissociated from mouse liver using a tissue grinder device or by enzymatic dissociation. The datasets include two runs from each experiment.

Measurements were performed using real-time fluorescent deformability cytometry.
TG.zip-contains data acquired during the measurements in 'rtdc' format from tissues dissociated using a tissue grinder.
Enzymatic.zip-contains data acquired during the measurements in 'rtdc' format from tissues dissociated using an enzymatic protocol.

This dataset contains measurements of the physical phenotype of mouse cells mechanically dissociated from the spleen, thymus, and kidney using a tissue grinder device.

Measurements were performed using real-time fluorescent deformability cytometry. Fluorescent antibodies used: CD31-PE, CD45-FITC and EpCAM-APC.
TG.zip-contains data acquired during the measurements in '.rtdc' format of spleen or thymus cells dissociated using a tissue grinder (TG) device).

This dataset contains measurements of the physical phenotype of mouse cells mechanically dissociated from the spleen, thymus, and kidney using a tissue grinder device.

Measurements were performed using real-time fluorescent deformability cytometry. Fluorescent antibodies used: CD31-PE, CD45-FITC and EpCAM-APC.
TG.zip-contains data acquired during the measurements in '.rtdc' format of spleen or thymus cells dissociated using a tissue grinder (TG) device).

This dataset contains measurements of the physical phenotype of single cells dissociated from mouse liver using a tissue grinder device or by enzymatic dissociation. The datasets include two runs from each experiment.

Measurements were performed using real-time fluorescent deformability cytometry.
TG.zip-contains data acquired during the measurements in 'rtdc' format from tissues dissociated using a tissue grinder.
Enzymatic.zip-contains data acquired during the measurements in 'rtdc' format from tissues dissociated using an enzymatic protocol.

Clinical syndrome coronavirus disease 2019 (COVID-19) induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is characterized by rapid spreading and high mortality worldwide. While the pathology is not yet fully understood, hyper-inflammatory response and coagulation disorders leading to congestions of microvessels are considered to be key drivers of the still increasing death toll. Until now, physical changes of blood cells have not been considered to play a role in COVID-19 related vascular occlusion and organ damage. Here we report an evaluation of multiple physical parameters including the mechanical features of five frequent blood cell types, namely erythrocytes, lymphocytes, monocytes, neutrophils, and eosinophils. More than 4 million blood cells of 17 COVID-19 patients at different levels of severity, 24 volunteers free from infectious or inflammatory diseases, and 14 recovered COVID-19 patients were analyzed. We found significant changes in lymphocyte stiffness, monocyte size, neutrophil size and deformability, and heterogeneity of erythrocyte deformation and size. While some of these changes recovered to normal values after hospitalization, others persisted for months after hospital discharge, evidencing the long-term imprint of COVID-19 on the body.

Clinical syndrome coronavirus disease 2019 (COVID-19) induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is characterized by rapid spreading and high mortality worldwide. While the pathology is not yet fully understood, hyper-inflammatory response and coagulation disorders leading to congestions of microvessels are considered to be key drivers of the still increasing death toll. Until now, physical changes of blood cells have not been considered to play a role in COVID-19 related vascular occlusion and organ damage. Here we report an evaluation of multiple physical parameters including the mechanical features of five frequent blood cell types, namely erythrocytes, lymphocytes, monocytes, neutrophils, and eosinophils. More than 4 million blood cells of 17 COVID-19 patients at different levels of severity, 24 volunteers free from infectious or inflammatory diseases, and 14 recovered COVID-19 patients were analyzed. We found significant changes in lymphocyte stiffness, monocyte size, neutrophil size and deformability, and heterogeneity of erythrocyte deformation and size. While some of these changes recovered to normal values after hospitalization, others persisted for months after hospital discharge, evidencing the long-term imprint of COVID-19 on the body.

This dataset contains measurements of the physical phenotype of mouse cells mechanically dissociated from the mouse colon using a tissue grinder device. The datasets include a control group, i.e. healthy mouse colon and a transfer colitis group; where immunodeficient mice were injected intraperitoneally with CD4+CD25- T cells three weeks prior to extracting and analysing colon cells.

Measurements were performed using real-time fluorescent deformability cytometry. Fluorescent antibodies used: CD31-PE, CD45-FITC and EpCAM-APC.
.zip-contains data acquired during the measurements in 'rtdc' format

This dataset contains measurements of the physical phenotype of single cells mechanically dissociated from mouse liver, colon and kidney using a tissue grinder device.

Measurements were performed using real-time fluorescent deformability cytometry. Fluorescent antibodies used: CD31-PE, CD45-FITC and EpCAM-APC.
.rtdc-contains data acquired during the measurements in 'rtdc' format.

This dataset contains measurements of the physical phenotype of single cells mechanically dissociated from mouse liver, colon and kidney using a tissue grinder device.

Measurements were performed using real-time fluorescent deformability cytometry. Fluorescent antibodies used: CD31-PE, CD45-FITC and EpCAM-APC.
.rtdc-contains data acquired during the measurements in 'rtdc' format.

This dataset contains measurements of the physical phenotype of mouse cells mechanically dissociated from the mouse colon using a tissue grinder device. The datasets include a control group, i.e. healthy mouse colon and a transfer colitis group; where immunodeficient mice were injected intraperitoneally with CD4+CD25- T cells three weeks prior to extracting and analysing colon cells.

Measurements were performed using real-time fluorescent deformability cytometry. Fluorescent antibodies used: CD31-PE, CD45-FITC and EpCAM-APC.
.zip-contains data acquired during the measurements in 'rtdc' format