Cortical circuits display different functional states under the influence of subcortical modulatory inputs. In our study we aimed to uncover fundamental aspects of this subcortical modulation by focusing on the effect of vGlut3 positive median raphe neurons on the hippocampal circuitry. Single neuron activity as well as population patterns were recorded the dorsal CA1 region in head-restrained, behaving mice, while vGlut3 positive raphe-hippocampal terminals or their source neurons in the median raphe were stimulated. This dataset complements our larger database of hippocampal neurons recorded and filled in hippocampal slices, mainly by characterizing the spontaneous and evoked physiological behavior of the cells under in vivo conditions.

Cortical circuits display different functional states under the influence of subcortical modulatory input. In our study we aimed to uncover fundamental aspects of this subcortical modulation by focusing on the effect of the median raphe nucleus on the hippocampal network. High channel count electrophysiological recordings were executed from dorsal hippocampus, along with the measurement of neuronal activity in the median raphe, in head-restrained, awake mice. The different cell types of the MR (vGAT, vGlut2, vGlut3) were optogenetically identified and multimodal sensory stimuli were applied to the mouse while monitoring both subcortical activity in the MR and activity in the dorsal CA1 region. These data allow us to characterize the properties of hippocampal neurons in the intact animal and how these properties are changed by subcortical inputs thus providing important constraints for network models of the hippocampus.

This study has provided both intracellular recordings from single neurons and LFP recordings from the hippocampal CA1 region in head-restrained, behaving mice, along with morphological reconstructions of some of the recorded cells. This dataset complements our larger database of hippocampal neurons recorded and filled in hippocampal slices, mainly by characterizing the spontaneous and evoked physiological behavior of the cells under in vivo conditions.