Additional file 1: Table S1. Tobacco AQP pseudo genes. Table of sequences that encode for incomplete AQPs within the tobacco TN90 genome sequence (Sierro et al. 2014), that we have subsequently assigned as pseudo genes. Notes on trans-membrane domains were sourced from analysis using TOPCONs protein topology prediction software. Table S2. Extended information on the 76 tobacco aquaporins identified in this study. Provided are protein lengths, gene identifiers, gene structures, chromosome and/or scaffold locations in the TN90 [1] (Sierro et al. 2014) and K326 [2] (Edwards et al. 2017) cultivar genomes, comparison of whether the computed gene models derived from each study matched gene structures curated in this study (Y-yes or N-no) and NCBI accessions. NtTIP2;5 s, NtNIP4;2 s and NtNIP4;3 t genes were not identified in the K326 [2] cultivar’s genome. Also listed are the corresponding tomato and potato orthologs and their respective gene (inton/exon) structures. Table S3. Amended annotations of previously reported tomato, potato and tobacco AQPs. In analysing the NtAQP family, we identified misannotations in previously reported AQPs from tomato (Solanum lycopersicum), potato (Solanum tuberosum) and tobacco (Nicotiana tabacum). Provided is a brief description of the error. Corrected sequences can be found in Additional file 3.

Additional file 1: Table S1. Tobacco AQP pseudo genes. Table of sequences that encode for incomplete AQPs within the tobacco TN90 genome sequence (Sierro et al. 2014), that we have subsequently assigned as pseudo genes. Notes on trans-membrane domains were sourced from analysis using TOPCONs protein topology prediction software. Table S2. Extended information on the 76 tobacco aquaporins identified in this study. Provided are protein lengths, gene identifiers, gene structures, chromosome and/or scaffold locations in the TN90 [1] (Sierro et al. 2014) and K326 [2] (Edwards et al. 2017) cultivar genomes, comparison of whether the computed gene models derived from each study matched gene structures curated in this study (Y-yes or N-no) and NCBI accessions. NtTIP2;5 s, NtNIP4;2 s and NtNIP4;3 t genes were not identified in the K326 [2] cultivar’s genome. Also listed are the corresponding tomato and potato orthologs and their respective gene (inton/exon) structures. Table S3. Amended annotations of previously reported tomato, potato and tobacco AQPs. In analysing the NtAQP family, we identified misannotations in previously reported AQPs from tomato (Solanum lycopersicum), potato (Solanum tuberosum) and tobacco (Nicotiana tabacum). Provided is a brief description of the error. Corrected sequences can be found in Additional file 3.

Additional file 3: Repository of sequences examined in this study. Genomic (gff3 format), CDS, and protein sequences for all 76 NtAQPs. CDS and protein sequences for all N.sylvestris and N.tomentosiformis AQPs. Amended sequences for potato StXIP3;1, StXIP4;1, and tomato SlXIP1;6, SlPIP2;1, SlTIP2;2 proteins (see also Additional file 1: Table S3)

Additional file 3: Repository of sequences examined in this study. Genomic (gff3 format), CDS, and protein sequences for all 76 NtAQPs. CDS and protein sequences for all N.sylvestris and N.tomentosiformis AQPs. Amended sequences for potato StXIP3;1, StXIP4;1, and tomato SlXIP1;6, SlPIP2;1, SlTIP2;2 proteins (see also Additional file 1: Table S3)