Automated Author ProfileBetsou, Fay
Betsou, Fay
Current S-Index
Sum of Dataset Indices for all datasets
Average Dataset Index per Dataset
Average Dataset Index per dataset
Total Datasets
Total datasets for this author
Average FAIR Score
Average FAIR Score per dataset
Total Citations
Total citations to the author's datasets
Total Mentions
Total mentions of the author's datasets
S-Index Interpretation
The S-Index (Sharing Index) is a comprehensive metric that represents the cumulative impact of all your datasets. It is calculated as the sum of Dataset Index scores across all your claimed datasets.
What it means:
- A higher S-index indicates greater overall impact of your datasets relative to typical datasets in their fields of research
- The S-Index grows as you add more datasets or as existing datasets gain more citations and mentions
- It provides a single number to track your research data impact over time
Current S-Index: 6.9 (sum of 10 datasets Dataset Index scores)
More information here.
S-Index Over Time
Cumulative Citations Over Time
Cumulative Mentions Over Time
Datasets
The successful use of human induced pluripotent stem cells (iPSCs) for research or clinical applications requires the development of robust, efficient, and reproducible cryopreservation protocols. After cryopreservation, the survival rate of iPSCs is suboptimal and cell line dependent. We assessed the use of ice recrystallization inhibitors (IRIs) for cryopreservation of human iPSCs. A toxicity screening study was performed to assess specific small-molecule carbohydrate-based IRI and concentrations for further evaluation. Then, a cryopreservation study compared the cryoprotective efficiency of 15 mM IRIs in 5 % or 10 % DMSO-containing solutions and with CryoStor® CS10. Three iPSC lines were cryopreserved as single-cell suspensions in the cryopreservation solutions and post-thaw characteristics, including pluripotency and differential gene expression, were assessed. We demonstrate the fitness-for-purpose of 15 mM IRI in 5 % DMSO as an efficient cryoprotective solution for iPSCs in terms of post-thaw recovery, viability, pluripotency, and transcriptomic changes. Given that this dataset is the first report where mRNA sequencing has been used to identify expression changes resulting from iPSCs cryopreservation, it has the potential to be used for molecular mechanism analysis relating to cryopreservation. IRIs can reduce DMSO concentrations, thereby improving the utility, effectiveness, and efficiency of cryopreservation.
Authors
- Mommaerts, Kathleen ;
- Okawa, Satoshi ;
- Schmitt, Margaux ;
- Kofanova, Olga ;
- Turner, Tracey ;
- Ben, Robert ;
- Del Sol, Antonio ;
- Schwamborn, Jens ;
- Acker, Jason ;
- Betsou, Fay
The successful use of human induced pluripotent stem cells (iPSCs) for research or clinical applications requires the development of robust, efficient, and reproducible cryopreservation protocols. After cryopreservation, the survival rate of iPSCs is suboptimal and cell line dependent. We assessed the use of ice recrystallization inhibitors (IRIs) for cryopreservation of human iPSCs. A toxicity screening study was performed to assess specific small-molecule carbohydrate-based IRI and concentrations for further evaluation. Then, a cryopreservation study compared the cryoprotective efficiency of 15 mM IRIs in 5 % or 10 % DMSO-containing solutions and with CryoStor® CS10. Three iPSC lines were cryopreserved as single-cell suspensions in the cryopreservation solutions and post-thaw characteristics, including pluripotency and differential gene expression, were assessed. We demonstrate the fitness-for-purpose of 15 mM IRI in 5 % DMSO as an efficient cryoprotective solution for iPSCs in terms of post-thaw recovery, viability, pluripotency, and transcriptomic changes. Given that this dataset is the first report where mRNA sequencing has been used to identify expression changes resulting from iPSCs cryopreservation, it has the potential to be used for molecular mechanism analysis relating to cryopreservation. IRIs can reduce DMSO concentrations, thereby improving the utility, effectiveness, and efficiency of cryopreservation.
Authors
- Mommaerts, Kathleen ;
- Okawa, Satoshi ;
- Schmitt, Margaux ;
- Kofanova, Olga ;
- Turner, Tracey ;
- Ben, Robert ;
- Del Sol, Antonio ;
- Schwamborn, Jens ;
- Acker, Jason ;
- Betsou, Fay
Additional file 3. Summary statistics of the genus phenotype association analyses.
Authors
- Baldini, Federico ;
- Hertel, Johannes ;
- Sandt, Estelle ;
- Thinnes, Cyrille C. ;
- Lorieza Neuberger-Castillo ;
- Pavelka, Lukas ;
- Betsou, Fay ;
- Rejko Krüger ;
- Thiele, Ines
Additional file 3. Summary statistics of the genus phenotype association analyses.
Authors
- Thiele, Ines ;
- Baldini, Federico ;
- Hertel, Johannes ;
- Sandt, Estelle ;
- Thinnes, Cyrille C. ;
- Lorieza Neuberger-Castillo ;
- Pavelka, Lukas ;
- Betsou, Fay ;
- Rejko Krüger
Additional file 4. Summary statistics for the variance contributions of genera to net production capacities.
Authors
- Baldini, Federico ;
- Hertel, Johannes ;
- Sandt, Estelle ;
- Thinnes, Cyrille C. ;
- Lorieza Neuberger-Castillo ;
- Pavelka, Lukas ;
- Betsou, Fay ;
- Rejko Krüger ;
- Thiele, Ines
Additional file 2. Summary statistics of the species phenotype association analyses.
Authors
- Baldini, Federico ;
- Hertel, Johannes ;
- Sandt, Estelle ;
- Thinnes, Cyrille C. ;
- Lorieza Neuberger-Castillo ;
- Pavelka, Lukas ;
- Betsou, Fay ;
- Rejko Krüger ;
- Thiele, Ines
Additional file 4. Summary statistics for the variance contributions of genera to net production capacities.
Authors
- Baldini, Federico ;
- Hertel, Johannes ;
- Sandt, Estelle ;
- Thinnes, Cyrille C. ;
- Lorieza Neuberger-Castillo ;
- Pavelka, Lukas ;
- Betsou, Fay ;
- Rejko Krüger ;
- Thiele, Ines
Additional file 5. Summary statistics of the net production capacity phenotype association analyses.
Authors
- Baldini, Federico ;
- Hertel, Johannes ;
- Sandt, Estelle ;
- Thinnes, Cyrille C. ;
- Lorieza Neuberger-Castillo ;
- Pavelka, Lukas ;
- Betsou, Fay ;
- Rejko Krüger ;
- Thiele, Ines
Additional file 5. Summary statistics of the net production capacity phenotype association analyses.
Authors
- Baldini, Federico ;
- Hertel, Johannes ;
- Sandt, Estelle ;
- Thinnes, Cyrille C. ;
- Lorieza Neuberger-Castillo ;
- Pavelka, Lukas ;
- Betsou, Fay ;
- Rejko Krüger ;
- Thiele, Ines
Additional file 2. Summary statistics of the species phenotype association analyses.
Authors
- Baldini, Federico ;
- Hertel, Johannes ;
- Sandt, Estelle ;
- Thinnes, Cyrille C. ;
- Lorieza Neuberger-Castillo ;
- Pavelka, Lukas ;
- Betsou, Fay ;
- Rejko Krüger ;
- Thiele, Ines