Additional file 9 Extraction of detailed information on the annotated genes in the goldfish sex determination regions [SDR (N = 373)] extracted from the NCBI goldfish female genome assembly annotation file (accession number QPKE00000000).

Additional file 6 Sequences of putative Y-allele RAD-tags (N = 32) found in some males but absent from all females.

Additional file 6 Sequences of putative Y-allele RAD-tags (N = 32) found in some males but absent from all females.

Additional file 7. Contig names (contigID) from a goldfish Illumina male genome assembly with homologies with the putative Y-allele RAD-tags.

Additional file 7. Contig names (contigID) from a goldfish Illumina male genome assembly with homologies with the putative Y-allele RAD-tags.

Additional file 8. Annotation of potential Y chromosome contigs found in our male goldfish draft genome assembly by sequence comparisons using blastx searches for genes by BLAST.

Additional file 8. Annotation of potential Y chromosome contigs found in our male goldfish draft genome assembly by sequence comparisons using blastx searches for genes by BLAST.

Additional file 9 Extraction of detailed information on the annotated genes in the goldfish sex determination regions [SDR (N = 373)] extracted from the NCBI goldfish female genome assembly annotation file (accession number QPKE00000000).

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

In the past two decades, bats have emerged as an important model system to study host-pathogen interactions. More recently, it has been shown that bats may also serve as a new and excellent model to study aging, inflammation and cancer among other important biological processes. The cave nectar bat or lesser dawn bat (Eonycteris spelaea), is known to be a reservoir for several viruses and intracellular bacteria. It is a widely-distributed bat species throughout the tropics and subtropics from India to Southeast Asia, and pollinates several plant species, including the culturally and economically important durian in the region. Here, we report the whole-genome and transcriptome sequencing, followed by subsequent de novo assembly of the E. spelaea genome solely using the PacBio® long-read sequencing platform.
The newly assembled E. spelaea genome is 1.97 Gb in length and consists of 4,470 sequences with a contig N50 of 8.0 Mb. Identified repeat elements covered 34.65% of the genome and 20,640 unique protein coding genes with 39,526 transcripts were annotated.
We demonstrated that PacBio long-read sequencing platform alone is sufficient to generate a comprehensive de novo assembled genome and transcriptome of an important bat species. These results will provide useful insights and act as a resource to expand our understanding of bat evolution, ecology, physiology, immunology, viral infection and transmission dynamics.