Background: Mitochondrial ATP production, essential for cardiomyocyte function, is regulated by mitochondrial Ca²⁺ (mtCa²⁺). The primary route for mtCa²⁺ influx is the mitochondrial calcium uniporter complex (mtCU). The mtCU subunit Mitochondrial Calcium Uptake 1 (MICU1) limits mtCa²⁺ uptake, preventing mtCa²⁺ overload. Although elevated mtCa²⁺ has been observed in multiple diseases including heart failure, its effects on heart function remain elusive.Methods: To investigate the impact of elevated mtCa²⁺ in adult hearts, we generated a mouse model with cardiomyocyte-specific tamoxifen-inducible Micu1 deletion (Micu1^cKO). Cardiac function was assessed through echocardiography. Mitochondria, adult cardiomyocytes, and tissue extracts were isolated from the left ventricle (LV) and right ventricle (RV) for comprehensive analysis at multiple timepoints ranging from 1 week to 9 weeks post-tamoxifen injection.

Background: Mitochondrial ATP production, essential for cardiomyocyte function, is regulated by mitochondrial Ca²⁺ (mtCa²⁺). The primary route for mtCa²⁺ influx is the mitochondrial calcium uniporter complex (mtCU). The mtCU subunit Mitochondrial Calcium Uptake 1 (MICU1) limits mtCa²⁺ uptake, preventing mtCa²⁺ overload. Although elevated mtCa²⁺ has been observed in multiple diseases including heart failure, its effects on heart function remain elusive.Methods: To investigate the impact of elevated mtCa²⁺ in adult hearts, we generated a mouse model with cardiomyocyte-specific tamoxifen-inducible Micu1 deletion (Micu1^cKO). Cardiac function was assessed through echocardiography. Mitochondria, adult cardiomyocytes, and tissue extracts were isolated from the left ventricle (LV) and right ventricle (RV) for comprehensive analysis at multiple timepoints ranging from 1 week to 9 weeks post-tamoxifen injection.

Background: Mitochondrial ATP production, essential for cardiomyocyte function, is regulated by mitochondrial Ca²⁺ (mtCa²⁺). The primary route for mtCa²⁺ influx is the mitochondrial calcium uniporter complex (mtCU). The mtCU subunit Mitochondrial Calcium Uptake 1 (MICU1) limits mtCa²⁺ uptake, preventing mtCa²⁺ overload. Although elevated mtCa²⁺ has been observed in multiple diseases including heart failure, its effects on heart function remain elusive.Methods: To investigate the impact of elevated mtCa²⁺ in adult hearts, we generated a mouse model with cardiomyocyte-specific tamoxifen-inducible Micu1 deletion (Micu1^cKO). Cardiac function was assessed through echocardiography. Mitochondria, adult cardiomyocytes, and tissue extracts were isolated from the left ventricle (LV) and right ventricle (RV) for comprehensive analysis at multiple timepoints ranging from 1 week to 9 weeks post-tamoxifen injection.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

Background: Mitochondrial ATP production, essential for cardiomyocyte function, is regulated by mitochondrial Ca²⁺ (mtCa²⁺). The primary route for mtCa²⁺ influx is the mitochondrial calcium uniporter complex (mtCU). The mtCU subunit Mitochondrial Calcium Uptake 1 (MICU1) limits mtCa²⁺ uptake, preventing mtCa²⁺ overload. Although elevated mtCa²⁺ has been observed in multiple diseases including heart failure, its effects on heart function remain elusive.Methods: To investigate the impact of elevated mtCa²⁺ in adult hearts, we generated a mouse model with cardiomyocyte-specific tamoxifen-inducible Micu1 deletion (Micu1^cKO). Cardiac function was assessed through echocardiography. Mitochondria, adult cardiomyocytes, and tissue extracts were isolated from the left ventricle (LV) and right ventricle (RV) for comprehensive analysis at multiple timepoints ranging from 1 week to 9 weeks post-tamoxifen injection.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.