This Dataset contains all the Source Data corresponding to the results from the manuscript entitled "A Protein Misfolding Shaking Amplification-based method for the spontaneous generation of hundreds of infectious prions", accepted for publication in Nature Communications Journal. The dataset includes uncropped scans of all gels and blots used to generated the final figures and tables (including Supplementary figures and tables), as well as the raw data used to generate the plots shown in the manuscript.The dataset is organized by Figures and Tables, with the source data files containing all the information used in each figure or table included in a compressed (.zip) file. The figure and table numbering used in the manuscript is kept, with source data available for: Figures 1 & 2, Figure 3, Supplementary figure 1, Supplementary figure 2,  Supplementary figure 3, and Supplementary figure 4.Manuscript abstract:Prion diseases are a group of rapidly progressing neurodegenerative disorders caused by the misfolding of the endogenous prion protein (PrPC) into a pathogenic form (PrPSc). This process, despite being the central event underlying these disorders, remains largely unknown at a molecular level, precluding the prediction of new potential outbreaks or interspecies transmission incidents.In this work, we present a method to generate infectious bona fide recombinant prions de novo, allowing a comprehensive analysis of protein misfolding across a wide range of prion proteins from mammalian species. We studied more than 380 different prion proteins from mammals and classified them according to their spontaneous misfolding propensity and their conformational variability.This study aims to address fundamental questions in the prion research field such as defining infectivity determinants, interspecies transmission barriers or the structural influence of specific amino acids and provides invaluable information for future diagnosis and therapy applications.

This Dataset contains all the Source Data corresponding to the results from the manuscript entitled "A Protein Misfolding Shaking Amplification-based method for the spontaneous generation of hundreds of infectious prions", accepted for publication in Nature Communications Journal. The dataset includes uncropped scans of all gels and blots used to generated the final figures and tables (including Supplementary figures and tables), as well as the raw data used to generate the plots shown in the manuscript.The dataset is organized by Figures and Tables, with the source data files containing all the information used in each figure or table included in a compressed (.zip) file. The figure and table numbering used in the manuscript is kept, with source data available for: Figures 1 & 2, Figure 3, Supplementary figure 1, Supplementary figure 2,  Supplementary figure 3, and Supplementary figure 4.Manuscript abstract:Prion diseases are a group of rapidly progressing neurodegenerative disorders caused by the misfolding of the endogenous prion protein (PrPC) into a pathogenic form (PrPSc). This process, despite being the central event underlying these disorders, remains largely unknown at a molecular level, precluding the prediction of new potential outbreaks or interspecies transmission incidents.In this work, we present a method to generate infectious bona fide recombinant prions de novo, allowing a comprehensive analysis of protein misfolding across a wide range of prion proteins from mammalian species. We studied more than 380 different prion proteins from mammals and classified them according to their spontaneous misfolding propensity and their conformational variability.This study aims to address fundamental questions in the prion research field such as defining infectivity determinants, interspecies transmission barriers or the structural influence of specific amino acids and provides invaluable information for future diagnosis and therapy applications.

Additional file 2 SESC-SDPV cases summary including information about age, breed, sex, organ, gross findings, meat inspector suspicion and final diagnosis. NA = Not Available; N/A = Not applicable; Piglet = 1.5 to 3 months; Fattening = 4 months to 1 year; Adult (sow) = Breeding female; Adult (boar) = Breeding male; F=Female; M = Male; (+) = positive; (−) = negative; DLBCL = Diffuse large B-cell Lymphoma, unspecified; DLBCL-CB=Diffuse large B-cell Lymphoma - Centroblastic subtype; B-SLL = B-cell Small lymphocytic lymphoma; PTCL = Peripheral T-cell Lymphomas, unspecified; Null cell = Non-T non B; B-LBL = B-lymphoblastic leukemia; UC=Unclassified.

Additional file 3 SESC-SDPV Lymphoma cases and classification following the current classification of lymphoid neoplasms for domestic animals adopted by the WHO. NA = Not Available; N/A = Not applicable; Piglet = 1.5 to 3 months; Fattening = 4 months to 1 year; Adult (sow) = Breeding female; Adult (boar) = Breeding male; F=Female; M = Male; LN = Lymph node; DLBCL = Diffuse large B-cell Lymphoma, unspecified; DLBCL-CB=Diffuse large B-cell Lymphoma - Centroblastic subtype; B-SLL = B-cell Small lymphocytic lymphoma; PTCL = Peripheral T-cell Lymphomas, unspecified; Null cell = Non-T non B; B-LBL = B-lymphoblastic leukemia; UC=Unclassified.

Additional file 1 Summary of the systematic literature review of the domestic pig (Sus scrofa domesticus) neoplasms reported between 1956 and 2021. Miniature breeds not included. NA = Not Available; d = days; m = months; w = weeks; y = years; F=Female; M = Male.

Additional file 3 SESC-SDPV Lymphoma cases and classification following the current classification of lymphoid neoplasms for domestic animals adopted by the WHO. NA = Not Available; N/A = Not applicable; Piglet = 1.5 to 3 months; Fattening = 4 months to 1 year; Adult (sow) = Breeding female; Adult (boar) = Breeding male; F=Female; M = Male; LN = Lymph node; DLBCL = Diffuse large B-cell Lymphoma, unspecified; DLBCL-CB=Diffuse large B-cell Lymphoma - Centroblastic subtype; B-SLL = B-cell Small lymphocytic lymphoma; PTCL = Peripheral T-cell Lymphomas, unspecified; Null cell = Non-T non B; B-LBL = B-lymphoblastic leukemia; UC=Unclassified.

Additional file 2 SESC-SDPV cases summary including information about age, breed, sex, organ, gross findings, meat inspector suspicion and final diagnosis. NA = Not Available; N/A = Not applicable; Piglet = 1.5 to 3 months; Fattening = 4 months to 1 year; Adult (sow) = Breeding female; Adult (boar) = Breeding male; F=Female; M = Male; (+) = positive; (−) = negative; DLBCL = Diffuse large B-cell Lymphoma, unspecified; DLBCL-CB=Diffuse large B-cell Lymphoma - Centroblastic subtype; B-SLL = B-cell Small lymphocytic lymphoma; PTCL = Peripheral T-cell Lymphomas, unspecified; Null cell = Non-T non B; B-LBL = B-lymphoblastic leukemia; UC=Unclassified.

Additional file 1 Summary of the systematic literature review of the domestic pig (Sus scrofa domesticus) neoplasms reported between 1956 and 2021. Miniature breeds not included. NA = Not Available; d = days; m = months; w = weeks; y = years; F=Female; M = Male.