This dataset contains Particulate Inorganic Carbon (PIC) concentrations from coccolithophore cultures, and associated metadata, for evaluating sample processing protocols. These data were collected on polycarbonate filters, with two different filter pore sizes, and two different filter rinse types. To achieve a range of  PIC concentrations, we sampled five different culture strains with diverse coccolith morphologies. For more details on dataset preparation and processing, see the Original Publication Citation and Data Processing Workflow below.

This dataset contains Particulate Inorganic Carbon (PIC) concentrations from coccolithophore cultures, and associated metadata, for evaluating sample processing protocols. These data were collected on polycarbonate filters, with two different filter pore sizes, and two different filter rinse types. To achieve a range of  PIC concentrations, we sampled five different culture strains with diverse coccolith morphologies. For more details on dataset preparation and processing, see the Original Publication Citation and Data Processing Workflow below.

Field experiments were performed in the NW Atlantic during R/V Endeavor cruise EN616 in July 2018 to determine the potential uptake of dissolved organic carbon (DOC) by coccolithophores to compare with the bicarbonate uptake associated with standard photosynthesis and calcification. Waters from the continental shelf and slope, plus Sargasso Sea were sampled. Natural phytoplankton populations were incubated with radiolabeled dissolved organic carbon compounds (14C-acetate, 14C-mannitol, and 14C-glycerol) as well as 14C-bicarbonate for comparison. At the end of 24-hour incubations, phytoplankton were either bulk filtered onto polycarbonate membrane filters or coccolithophores were concentrated, then sorted, using a flow cytometer, after which sorted cells were washed onto polycarbonate membrane filters and rinsed accordingly. Post-cruise, repeated scintillation counts of each sample were performed using a high sensitivity scintillation counter. The repeated counts were designed to improve the statistical resolution of the low-level radioactivity measurements. Post-cruise, ambient concentrations of acetate, mannitol, and glycerol also were measured and cellular-DOC uptake rates were calculated.

Hydrography and environmental conditions were measured with CTD at nine stations during R/V Endeavor cruise EN616 in July 2018. The stations ranged from the New England Continental Shelf, New England Continental Slope, to the Sargasso Sea ocean regions.

This dataset presents imaging cytometer data from water samples collected during R/V Endeavor cruise EN616. Niskin bottle samples were taken at nine stations and eight depths in the northwest Atlantic in July 2018. A Yokogawa FlowCAM imaging cytometer was used to enumerate the major microalgal classes, and the particle size distribution function was estimated.

This data set provides ambient concentrations of three dissolved organic compound (acetate, glycerol and mannitol) measured from water samples taken during R/V Endeavor cruise EN616 in the northwest Atlantic in July 2018. These concentrations were derived using new analytical methods described in the below-referenced Science Advances manuscript by Balch et al.

This dataset presents polarized microscopy-derived concentration data for coccolithophores and detached coccoliths in samples collected from stations in the Northwest Atlantic during R/V Endeavor cruise EN616 in July 2018. Counts are based on image analysis of dark-field, cross-polarized views of filtered particulate matter. These counts take advantage of the birefringence property of calcium carbonate (particulate inorganic carbon) that it rotates the plane of linearly polarized incident light by 90 degrees. Incident light directed upwards, towards the microscope slide, is polarized 90 degrees with a linear polarizer. Particles are viewed from above the slide, through a second, linear polarizer filter held between the microscope stage and the camera which only accepts light that is polarized orthogonal to the lower polarizer. Calcium carbonate particles in the beam appear as bright dots of light. Image analysis software then analyzes the pattern of birefringence and enumerates only those particles with size and shape of coccolithophores or detached coccoliths.

Results for radiolabeled acetate, mannitol, and glycerol kinetic uptake and pulse-chase experiments for the species Cruciplacolithus neohelis (McIntyre & Bé) Reinhardt strain CCMP298 and Chrysotila carterae (Braarud & Fagerland) Andersen, Kim, Tittley & Yoon (NCMA lists the strain as Pleurochrysis carterae) strain CCMP3337.

Results for batch growth experiments that lasted for 30 days on two species, Cruciplacolithus neohelis (McIntyre & Bé) Reinhardt strain CCMP298 and Chrysotila carterae (Braarud & Fagerland) Andersen, Kim, Tittley & Yoon (NCMA lists the strain as Pleurochrysis carterae) strain CCMP3337, grown in darkness with the addition of acetate, mannitol, and glycerol in final concentrations of 10, 30, 100, 300 and 1000 µmol l−1. We performed these experiments to determine whether coccolithophores (CCMP298 and CCMP3337) can sustain themselves in darkness by using organic compounds as energy and/or carbon sources.

This dataset includes results for 14C-labeled uptake experiments determining uptake of specific dissolved organic compounds which showed high potential for osmotrophy. Experiments used the BioLog Eco-plates (BioLog, Haywood, CA, U.S.A.) and were conducted at Bigelow Laboratory for Ocean Sciences, East Boothbay, ME.