D19 and D46 human Immunoglobulin M (IgM) libraries, sequenced by Illumina Miseq and publised in the paper titled Adaptive immune receptor genotyping using the corecount program.The D19 and D46 datasets consists of bulk sequencing data from IgM libraries obtained by IgM specific cDNA synthesis and sequencing in forward and reverse directions using the Illumina MiSeq platform (2 x 300 bp kit). Further details will be available in Narang et al., 2023 (doi: 10.3389/fimmu.2023.1125884)

The dataset consists of bulk sequencing data from IgM libraries obtained by IgM specific cDNA synthesis and sequencing in forward and reverse directions using the Illumina MiSeq 2 x 300 bp kit. The samples from which the dataset is retrieved is a set of 14 peripheral blood mononuclear cells (PBMCs) samples collected seven months after the primary infection by SARS-CoV-2.
Further details regarding the samples are available in Pushparaj et al., 2022 (https://doi.org/10.1016/j.immuni.2022.12.005)
To apply for conditional access to this dataset please contact [email protected].

Immunoglobulin M (IgM) and immunoglobulin G (IgG) repertoire sequencing libraries from two donors who were first infected by index SARS-CoV-2, then vaccinated by the mRNA-1273 vaccine. We acquired longitudinal peripheral blood mononuclear cells (PBMCs) samples from each respective donor as follows: 11 or 14 days post-infection (acute timepoint), 32 or 37 days post-infection (convalescent timepoint), 160 or 169 days post-infection (pre-vax timepoint), and 9 or 7 days post-vaccination (post-vax timepoint). Three IgM libraries were generated from each donor for the Acute timepoint, as well as one IgG library per timepoint per donor for a total of 6 IgM and 6 IgG Rep-seq libraries.

The dataset contains the repertoire sequencing data from 45 adult volunteers.For each case 4 separate TCR repseq libraries were constructed after cDNA synthesis using a UMI containing primer located in the constant exon of the TRA, TRD, TRB and TRG genes respectively. In each case a 5’ multiplex set of primers specific for the leader regions of the V genes of either TRA, TRD, TRB or TRG genes was used along with a universal reverse primer, introduced during the cDNA synthesis step to produce the gene specific repseq library.The libraries were sequenced using the Illumina 2 x 300 cycle MiSeq system at the Karlsson Hedestam Laboratory at the Department of Microbiology, Tumor and Cell Biology at Biomedicum, Karolinska Institutet, Stockholm.

Terms for accessThe datasets are only to be used for research that is seeking to advance the understanding of the influence of TCR genetic variation on human biology.

Immunoglobulin M (IgM) and immunoglobulin G (IgG) repertoire sequencing libraries from two donors who were first infected by index SARS-CoV-2, then vaccinated by the mRNA-1273 vaccine. We acquired longitudinal peripheral blood mononuclear cells (PBMCs) samples from each respective donor as follows: 11 or 14 days post-infection (acute timepoint), 32 or 37 days post-infection (convalescent timepoint), 160 or 169 days post-infection (pre-vax timepoint), and 9 or 7 days post-vaccination (post-vax timepoint). Three IgM libraries were generated from each donor for the Acute timepoint, as well as one IgG library per timepoint per donor for a total of 6 IgM and 6 IgG Rep-seq libraries.

D19 and D46 human Immunoglobulin M (IgM) libraries, sequenced by Illumina Miseq and publised in the paper titled Adaptive immune receptor genotyping using the corecount program.The D19 and D46 datasets consists of bulk sequencing data from IgM libraries obtained by IgM specific cDNA synthesis and sequencing in forward and reverse directions using the Illumina MiSeq platform (2 x 300 bp kit). Further details will be available in Narang et al., 2023 (doi: 10.3389/fimmu.2023.1125884)

The dataset consists of bulk sequencing data from IgM libraries obtained by IgM specific cDNA synthesis and sequencing in forward and reverse directions using the Illumina MiSeq 2 x 300 bp kit. The samples from which the dataset is retrieved is a set of 14 peripheral blood mononuclear cells (PBMCs) samples collected seven months after the primary infection by SARS-CoV-2.
Further details regarding the samples are available in Pushparaj et al., 2022 (https://doi.org/10.1016/j.immuni.2022.12.005)
To apply for conditional access to this dataset please contact [email protected].

The dataset contains the repertoire sequencing data from 45 adult volunteers.For each case 4 separate TCR repseq libraries were constructed after cDNA synthesis using a UMI containing primer located in the constant exon of the TRA, TRD, TRB and TRG genes respectively. In each case a 5’ multiplex set of primers specific for the leader regions of the V genes of either TRA, TRD, TRB or TRG genes was used along with a universal reverse primer, introduced during the cDNA synthesis step to produce the gene specific repseq library.The libraries were sequenced using the Illumina 2 x 300 cycle MiSeq system at the Karlsson Hedestam Laboratory at the Department of Microbiology, Tumor and Cell Biology at Biomedicum, Karolinska Institutet, Stockholm.

Terms for accessThe datasets are only to be used for research that is seeking to advance the understanding of the influence of TCR genetic variation on human biology.

The data contains two paired-end read files as fastq.gz per individual library. The sequencing files are in a folder "1" in a folder of the individual labelled "RA_ID_IGM". 
The libraries were made with an Immunoglobulin M reverse primer and a 5' mulitplex primer set binding in the leader region. The libraries were analyzed with IgDiscover in default settings.
Data Access StatementThe data can be made available for research purposes after publication. The human data has to remain stored in secure places with restricted access. Please send a request with your institutional affiliation, the purpose of the data request and data management information. Access will be granted by the main authors. If you are affiliated with a Swedish institution access is given through Uppmax.

The data contains two paired-end read files as fastq.gz per individual library. The sequencing files are in a folder "1" in a folder of the individual labelled "RA_ID_IGM". 
The libraries were made with an Immunoglobulin M reverse primer and a 5' mulitplex primer set binding in the leader region. The libraries were analyzed with IgDiscover in default settings.
Data Access StatementThe data can be made available for research purposes after publication. The human data has to remain stored in secure places with restricted access. Please send a request with your institutional affiliation, the purpose of the data request and data management information. Access will be granted by the main authors. If you are affiliated with a Swedish institution access is given through Uppmax.