Dataset (MATLAB files) of holograms used in:Mikołaj Rogalski, Julianna Winnik, Julia Dudek, Piotr Arcab, Emilia Wdowiak, Paweł Matryba, Marzena Stefaniuk, Piotr Zdańkowski, Maciej Trusiak,“Gigavoxel‑Scale Multiple‑Scattering‑Aware Lensless Holotomography”, submitted 2025.ContentsFor each of the four samples – Colosseum, Inclined_phase_resolution_target, Mouse_brain_section_500um and Multilayer_3D_phase_test – the following files are provided:Off‑axis holograms stacks_holograms_540.mat (green illumination, λ = 540 nm)_holograms_640.mat (red illumination, λ = 640 nm)Each file contains a three-dimensional array with dimensions [Y, X, 180], where Y and X denote the pixel height and width of an individual hologram, and the third dimension indexes 180 angular projections acquired every 2° across a full 360° sweep of the illumination beam.On‑axis calibration hologram_in_line.mat (on‑axis illumination, λ = 540 nm)A single on‑axis hologram used exclusively for calibration purposes.Experimental detailsHolograms were acquired in a custom lensless holotomography setup. The system included a board-level CMOS camera (ALVIUM 1800 U‑2050 m, 2.4 µm pixel size), with the sample placed approximately 2 mm above the sensor surface. Illumination was provided by a Supercontinuum laser (NKT Photonics SuperK), coupled into a single-mode optical fibre (Thorlabs P1‑S405‑FC‑2, ~3 µm core diameter). The output fibre was mounted on a motorized rotation stage, allowing full 360° rotation of the illumination beam in 2° steps. The azimuthal angle was set between 35° and 40°.For each of the 180 angular positions, two off-axis Gabor holograms were captured using λ = 540 nm and λ = 640 nm light, yielding 360 images per dataset. Additionally, a single on-axis hologram at 540 nm was acquired for calibration purposes and excluded from the tomographic reconstruction.More information on calibration and the reconstruction procedure can be found in the referenced article.

Dataset (MATLAB files) of holograms used in:Mikołaj Rogalski, Julianna Winnik, Julia Dudek, Piotr Arcab, Emilia Wdowiak, Paweł Matryba, Marzena Stefaniuk, Piotr Zdańkowski, Maciej Trusiak,“Gigavoxel‑Scale Multiple‑Scattering‑Aware Lensless Holotomography”, submitted 2025.ContentsFor each of the four samples – Colosseum, Inclined_phase_resolution_target, Mouse_brain_section_500um and Multilayer_3D_phase_test – the following files are provided:Off‑axis holograms stacks_holograms_540.mat (green illumination, λ = 540 nm)_holograms_640.mat (red illumination, λ = 640 nm)Each file contains a three-dimensional array with dimensions [Y, X, 180], where Y and X denote the pixel height and width of an individual hologram, and the third dimension indexes 180 angular projections acquired every 2° across a full 360° sweep of the illumination beam.On‑axis calibration hologram_in_line.mat (on‑axis illumination, λ = 540 nm)A single on‑axis hologram used exclusively for calibration purposes.Experimental detailsHolograms were acquired in a custom lensless holotomography setup. The system included a board-level CMOS camera (ALVIUM 1800 U‑2050 m, 2.4 µm pixel size), with the sample placed approximately 2 mm above the sensor surface. Illumination was provided by a Supercontinuum laser (NKT Photonics SuperK), coupled into a single-mode optical fibre (Thorlabs P1‑S405‑FC‑2, ~3 µm core diameter). The output fibre was mounted on a motorized rotation stage, allowing full 360° rotation of the illumination beam in 2° steps. The azimuthal angle was set between 35° and 40°.For each of the 180 angular positions, two off-axis Gabor holograms were captured using λ = 540 nm and λ = 640 nm light, yielding 360 images per dataset. Additionally, a single on-axis hologram at 540 nm was acquired for calibration purposes and excluded from the tomographic reconstruction.More information on calibration and the reconstruction procedure can be found in the referenced article.

Dataset consists of 3D images of mouse penis after tissue clearing protocol developed by us (see paper for details). Imaging was performed with MuVi SPIM (Luxendo) with a Nikon CFI 10X 0.5 NA objective lens, resulting in voxel size of 0.87x0.87x2.0 micrometers (datasets 01 and 02). For filament tracing (03) dataset was downsampled.01_whole-organ-betaIIItubuline-staining against beta III tubuline (hdf5 BigDataViewer)02_whole-organ-TH-staining staining against a neuronal marker tyrosine hydroxylase (TH) (hdf5 BigDataViewer)03_betaIIItubuline_with_filament_tracing (Imaris)