a high-quality telomere-to-telomere (T2T) genome assembly of Bombax ceiba

a high-quality telomere-to-telomere (T2T) genome assembly of Bombax ceiba

The first telomere-to-telomere (T2T) genome assembly of Castanopsis orthacantha was reported in this study, an ecologically and economically significant keystone tree species endemic to subtropical evergreen forests of southwestern China. Integrating multi-platform sequencing data (Illumina, PacBio HiFi, ONT ultra-long reads) and Hi-C scaffolding, we generated a chromosome-scale assembly spanning 893.28 Mb with a contig N50 of 76.19 Mb. The annotation files (annotation file in gff format, cds fasta file, and protein fasta file) of Castanopsis orthacantha genome assembly have been uploaded.

a high-quality telomere-to-telomere (T2T) genome assembly of Bombax ceiba

The first telomere-to-telomere (T2T) genome assembly of Castanopsis orthacantha was reported in this study, an ecologically and economically significant keystone tree species endemic to subtropical evergreen forests of southwestern China. Integrating multi-platform sequencing data (Illumina, PacBio HiFi, ONT ultra-long reads) and Hi-C scaffolding, we generated a chromosome-scale assembly spanning 893.28 Mb with a contig N50 of 76.19 Mb. The annotation files (annotation file in gff format, cds fasta file, and protein fasta file) of Castanopsis orthacantha genome assembly have been uploaded.

Nitric oxide (NO) plays an important role as a signalling molecule in the biological system. Organoselenium-coated or grafted biomaterials have the potential to achieve controlled NO release as they can catalyse decomposition of endogenous S-nitrosothiols to NO. However, such biomaterials are often challenged by the loss of the catalytic sites, which can affect the stability in tissue repair applications. In this work, we prepare a diselenide-containing poly(ester urethane)urea (SePEUU) polymer with Se–Se in the backbone, which is further electrospun into fibrous membranes by blending with poly(ester urethane)urea (PEUU) without diselenide bonds. The presence of catalytic sites in the main chain demonstrates stable and long-lasting NO catalytic activity, while the porous structure of the fibrous membranes ensures uniform distribution of the catalytic sites and better contact with the donor-containing solution. PEUU/SePEUU50 in 50/50 mass ratio has a physiologically adapted rate of NO release, with a sustained generation of NO after exposure to PBS at 37 °C for 30 d. PEUU/SePEUU50 has a low hemolysis and protein adsorption, with mechanical properties in the wet state matching those of natural vascular tissues. It can promote the adhesion and proliferation of human umbilical vein endothelial cells in vitro and control the proliferation of vascular smooth muscle cells in the presence of NO generation. This study exhibits the electrospun fibrous membranes have potential for utilizing as hemocompatible biomaterials for regeneration of blood-contacting tissues. Electrospun membranes of diselenide-containing poly(ester urethane)urea exhibit stable catalytic activity of NO generation, which promotes human umbilical vein endothelial cell proliferation and control the proliferation of vascular smooth muscle cells for potential applications as blood-contacting biomaterials.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

A total of 785.68 Gb of sequencing data was generated from 137 samples of A. yunnanensis using RAD-seq.  The vcf file containing 19,673 high-quality SNP loci  was provided.