The aim of this study was to evaluate the effect of eight commercially available EO-derived compounds ((R)-(+)-limonene, (S)-(−)-limonene, sabinene, carvacrol, thymol, alpha-pinene (α-pinene), beta-pinene (β-pinene), and cinnamaldehyde) on the bone formation process in vitro to select the most promising natural agents that could potentially be used in the prevention or treatment of osteoporosis. Within this study, evaluation of cytotoxicity, cell proliferation, and osteogenic differentiation was performed with the use of mouse primary calvarial preosteoblasts (MC3T3-E1). Moreover, extracellular matrix (ECM) mineralization was determined using MC3T3-E1 cells and dog adipose tissue-derived mesenchymal stem cells (ADSCs). The two highest non-toxic concentrations of each of the compounds were selected and used for testing other activities. This is a dataset related to the recently published article (Biomedicines 2023, 11(4), 1095; https://doi.org/10.3390/biomedicines11041095).Folder: “MTT” contains the raw results of three repetitions of MTT cytotoxicity assay performed after 24 hours of culture. The results of the MTT test are expressed as OD values.Folder: “LDH” contains the raw results of three repetitions of LDH proliferation assay performed after 24 and 72 hours of culture. The results of the LDH test are expressed as OD values.Folder: “Osteogenic differentiation” contains the raw results of three repetitions of osteogenic markers level (type I collagen, bone alkaline phosphatase and osteocalcin) and total cellular proteins amount after 8 days and 21 days of culture. The results of the osteogenic markers and cellular proteins tests are expressed as OD values and as concentration of the compound expressed in ng/pg per ml.Folder: “ARS” contains the raw results of three repetitions of mineral deposition assay performed after 21 days of cultures. The results of the ARS test are expressed as OD values.Folder: “ARS staining” contains raw stereoscopic images of mineral deposits in the ECM.

The aim of this study was to evaluate the effect of eight commercially available EO-derived compounds ((R)-(+)-limonene, (S)-(−)-limonene, sabinene, carvacrol, thymol, alpha-pinene (α-pinene), beta-pinene (β-pinene), and cinnamaldehyde) on the bone formation process in vitro to select the most promising natural agents that could potentially be used in the prevention or treatment of osteoporosis. Within this study, evaluation of cytotoxicity, cell proliferation, and osteogenic differentiation was performed with the use of mouse primary calvarial preosteoblasts (MC3T3-E1). Moreover, extracellular matrix (ECM) mineralization was determined using MC3T3-E1 cells and dog adipose tissue-derived mesenchymal stem cells (ADSCs). The two highest non-toxic concentrations of each of the compounds were selected and used for testing other activities. This is a dataset related to the recently published article (Biomedicines 2023, 11(4), 1095; https://doi.org/10.3390/biomedicines11041095).Folder: “MTT” contains the raw results of three repetitions of MTT cytotoxicity assay performed after 24 hours of culture. The results of the MTT test are expressed as OD values.Folder: “LDH” contains the raw results of three repetitions of LDH proliferation assay performed after 24 and 72 hours of culture. The results of the LDH test are expressed as OD values.Folder: “Osteogenic differentiation” contains the raw results of three repetitions of osteogenic markers level (type I collagen, bone alkaline phosphatase and osteocalcin) and total cellular proteins amount after 8 days and 21 days of culture. The results of the osteogenic markers and cellular proteins tests are expressed as OD values and as concentration of the compound expressed in ng/pg per ml.Folder: “ARS” contains the raw results of three repetitions of mineral deposition assay performed after 21 days of cultures. The results of the ARS test are expressed as OD values.Folder: “ARS staining” contains raw stereoscopic images of mineral deposits in the ECM.

The aim of this research was to optimize the concentration of individual components of polymer–ceramic nanocomposite granules (nanofilled polymer composites) for application in orthopedics and maxillofacial surgery to fill small bone defects and stimulate the regeneration process. Two types of granules were made using nanohydroxyapatite (nanoHA) and chitosan-based matrix (agarose/chitosan or curdlan/chitosan), which served as binder for ceramic nanopowder. Different concentrations of the components (nanoHA and curdlan), foaming agent (sodium bicarbonate—NaHCO3), and chitosan solvent (acetic acid—CH3COOH) were tested during the production process. Agarose and chitosan concentrations were fixed to be 5% w/v and 2% w/v, respectively, based on our previous research. Subsequently, the produced granules were subjected to cytotoxicity testing (indirect and direct contact methods), microhardness testing (Young’s modulus evaluation), and microstructure analysis (porosity, specific surface area, and surface roughness) in order to identify the biomaterial with the most favorable properties.This is a dataset related to the recently published article (Molecules 2023, 28, 5238. https://doi.org/10.3390/molecules28135238).Folder: “Live_Dead” contains raw CLSM images of the cells on the granules after live/dead staining.Folder: “MTT” contains the raw results of three repetitions of MTT cytotoxicity assay performed after 24 and 48 hours of culture. The results of MTT test are expressed as OD values.Folder: “SSA” contains Microsoft Excel files with results of the total surface area measurement performed for each sample.Folder: “SEM” contains raw SEM images of tested granules.Folder: “Stereoscopic images” contains raw stereoscopic images of the granules.Folder: “Roughness” contains raw 3D images of granules surface and a Microsoft Excel file (Roughness.csv) contains the results of areal surface roughness of each tested granule expressed as Sa (µm). A Microsoft Word file (Microhardness test.docx) contains the results of the microhardness test. The results are expressed as Young’s modulus values (GPa) presented in tables for each sample.A Microsoft Excel file (MIP - Porosity.csv) contains the results of porosity evaluation for each tested granule.

The aim of this research was to optimize the concentration of individual components of polymer–ceramic nanocomposite granules (nanofilled polymer composites) for application in orthopedics and maxillofacial surgery to fill small bone defects and stimulate the regeneration process. Two types of granules were made using nanohydroxyapatite (nanoHA) and chitosan-based matrix (agarose/chitosan or curdlan/chitosan), which served as binder for ceramic nanopowder. Different concentrations of the components (nanoHA and curdlan), foaming agent (sodium bicarbonate—NaHCO3), and chitosan solvent (acetic acid—CH3COOH) were tested during the production process. Agarose and chitosan concentrations were fixed to be 5% w/v and 2% w/v, respectively, based on our previous research. Subsequently, the produced granules were subjected to cytotoxicity testing (indirect and direct contact methods), microhardness testing (Young’s modulus evaluation), and microstructure analysis (porosity, specific surface area, and surface roughness) in order to identify the biomaterial with the most favorable properties.This is a dataset related to the recently published article (Molecules 2023, 28, 5238. https://doi.org/10.3390/molecules28135238).Folder: “Live_Dead” contains raw CLSM images of the cells on the granules after live/dead staining.Folder: “MTT” contains the raw results of three repetitions of MTT cytotoxicity assay performed after 24 and 48 hours of culture. The results of MTT test are expressed as OD values.Folder: “SSA” contains Microsoft Excel files with results of the total surface area measurement performed for each sample.Folder: “SEM” contains raw SEM images of tested granules.Folder: “Stereoscopic images” contains raw stereoscopic images of the granules.Folder: “Roughness” contains raw 3D images of granules surface and a Microsoft Excel file (Roughness.csv) contains the results of areal surface roughness of each tested granule expressed as Sa (µm). A Microsoft Word file (Microhardness test.docx) contains the results of the microhardness test. The results are expressed as Young’s modulus values (GPa) presented in tables for each sample.A Microsoft Excel file (MIP - Porosity.csv) contains the results of porosity evaluation for each tested granule.

The aim of this study was to evaluate the effect of eight commercially available EO-derived compounds ((R)-(+)-limonene, (S)-(-)-limonene, sabinene, carvacrol, thymol, alpha-pinene, beta-pinene, and cinnamaldehyde) on the bone formation process in vitro to select the most promising natural agents that could potentially be used in the prevention or treatment of osteoporosis. Within this study, evaluation of cytotoxicity, cell proliferation, and osteogenic differentiation was performed with the use of mouse primary calvarial preosteoblasts (MC3T3-E1). Moreover, extracellular matrix (ECM) mineralization was determined using MC3T3-E1 cells and dog adipose tissue-derived mesenchymal stem cells (ADSCs).This is a dataset related to the recently published article (Biomedicines 2023, 11, 1095. https://doi.org/10.3390/biomedicines11041095).Each GraphPad Prism file (.pzf) contains the results of LDH total proliferation assay for individual essential oil compound (file name indicates specific compound). GraphPad Prism 8.1.2 Software was used to generate these files. In “Data Table” you should select the name of the compound to see the results obtained for 3 repetitions of the test. The results are expressed as a “cell number x 104”. First row shows results for “24 hours” time interval and second row shows results for “72 hours” time interval. Group A always shows control cells, whereas Group B and Group C show different concentration of tested essential oils-derived compound.Each Microsoft Excel file (.csv) contains the results of either ARS mineralization test or ELISA for osteogenic markers (file name indicates the type of osteogenic marker or the cell type used for ARS mineralization test). The results of ARS are expressed as OD values. ELISA results are shown as concentration of specific osteogenic marker. Column B indicates control cells, columns C-R show results for different concentrations of tested essential oils-derived compounds.Abbreviations:DT – doubling timeOC – osteocalcinMC3T3-E1 – mouse preosteoblastsADSCs – adipose tissue-derived mesenchymal stem cellsARS – Alizarin Red S staining

The aim of this study was to evaluate the effect of eight commercially available EO-derived compounds ((R)-(+)-limonene, (S)-(-)-limonene, sabinene, carvacrol, thymol, alpha-pinene, beta-pinene, and cinnamaldehyde) on the bone formation process in vitro to select the most promising natural agents that could potentially be used in the prevention or treatment of osteoporosis. Within this study, evaluation of cytotoxicity, cell proliferation, and osteogenic differentiation was performed with the use of mouse primary calvarial preosteoblasts (MC3T3-E1). Moreover, extracellular matrix (ECM) mineralization was determined using MC3T3-E1 cells and dog adipose tissue-derived mesenchymal stem cells (ADSCs).This is a dataset related to the recently published article (Biomedicines 2023, 11, 1095. https://doi.org/10.3390/biomedicines11041095).Each GraphPad Prism file (.pzf) contains the results of LDH total proliferation assay for individual essential oil compound (file name indicates specific compound). GraphPad Prism 8.1.2 Software was used to generate these files. In “Data Table” you should select the name of the compound to see the results obtained for 3 repetitions of the test. The results are expressed as a “cell number x 104”. First row shows results for “24 hours” time interval and second row shows results for “72 hours” time interval. Group A always shows control cells, whereas Group B and Group C show different concentration of tested essential oils-derived compound.Each Microsoft Excel file (.csv) contains the results of either ARS mineralization test or ELISA for osteogenic markers (file name indicates the type of osteogenic marker or the cell type used for ARS mineralization test). The results of ARS are expressed as OD values. ELISA results are shown as concentration of specific osteogenic marker. Column B indicates control cells, columns C-R show results for different concentrations of tested essential oils-derived compounds.Abbreviations:DT – doubling timeOC – osteocalcinMC3T3-E1 – mouse preosteoblastsADSCs – adipose tissue-derived mesenchymal stem cellsARS – Alizarin Red S staining