This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection.
This data is associated with Figure 1 of the paper in Nature Microbiology. It contains videos of vertically trapped Bacillus subtilis cells expressing fluorescent proteins FtsZ and PBP2B, showing single-molecule tracking of the latter around the division ring. It also contains data from further down the processing and analysis pipeline from the same videos.220303_slide2_5_MMStack_Default_ring34.ome corresponds to Fig. 1b, left panels.220309_6_MMStack_Default_ring5.ome corresponds to Fig. 1b, right panels.220316_5_MMStack_Default_561_denoise.ome_ring15 corresponds to Fig. 1c, top kymograph.220316_5_MMStack_Default_561_denoise.ome_ring14 corresponds to Fig. 1c, bottom kymograph.220412_slide2_6_MMStack_Default_561_denoise.ome_ring11 corresponds to Fig. 1d, top kymograph.220414_4_MMStack_Default_561_denoise.ome_ring3 corresponds to Fig. 1d, bottom kymograph.220809_sh147_phmm_30c_vercini_fosfomycin_slide2_006_561_denoise_ring1 corresponds to Fig. 1e, top kymograph.220809_sh147_phmm_30c_vercini_fosfomycin_slide2_001_561_denoise_ring8 corresponds to Fig. 1e, bottom kymograph.230613_sh147_speeds_phmm30_gauss_2nmsbin and 230605_sh147_speeds_phmm30_logscale correspond to Fig. 1f.230614_sh147_phmm30_penG_2foldedgauss_2nmsbin and 230605_sh147_speeds_phmm30_penG_logscale correspond to Fig. 1g.230614_sh147_phmm30_fosfomycin_2foldedgauss_2nmsbin and 230605_sh147_speeds_phmm30_fos_logscale correspond to Fig. 1h.Whitley_2024_Fig1_SourceData contains all the data used to produce histograms in Fig. 1f, 1g, and 1h.

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection.
This data is associated with Figure 1 of the paper in Nature Microbiology. It contains videos of vertically trapped Bacillus subtilis cells expressing fluorescent proteins FtsZ and PBP2B, showing single-molecule tracking of the latter around the division ring. It also contains data from further down the processing and analysis pipeline from the same videos.220303_slide2_5_MMStack_Default_ring34.ome corresponds to Fig. 1b, left panels.220309_6_MMStack_Default_ring5.ome corresponds to Fig. 1b, right panels.220316_5_MMStack_Default_561_denoise.ome_ring15 corresponds to Fig. 1c, top kymograph.220316_5_MMStack_Default_561_denoise.ome_ring14 corresponds to Fig. 1c, bottom kymograph.220412_slide2_6_MMStack_Default_561_denoise.ome_ring11 corresponds to Fig. 1d, top kymograph.220414_4_MMStack_Default_561_denoise.ome_ring3 corresponds to Fig. 1d, bottom kymograph.220809_sh147_phmm_30c_vercini_fosfomycin_slide2_006_561_denoise_ring1 corresponds to Fig. 1e, top kymograph.220809_sh147_phmm_30c_vercini_fosfomycin_slide2_001_561_denoise_ring8 corresponds to Fig. 1e, bottom kymograph.230613_sh147_speeds_phmm30_gauss_2nmsbin and 230605_sh147_speeds_phmm30_logscale correspond to Fig. 1f.230614_sh147_phmm30_penG_2foldedgauss_2nmsbin and 230605_sh147_speeds_phmm30_penG_logscale correspond to Fig. 1g.230614_sh147_phmm30_fosfomycin_2foldedgauss_2nmsbin and 230605_sh147_speeds_phmm30_fos_logscale correspond to Fig. 1h.Whitley_2024_Fig1_SourceData contains all the data used to produce histograms in Fig. 1f, 1g, and 1h.

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. 
Whitley_2024_Fig2_SourceData contains all the data used to produce the plots in Fig. 2.231105_sh147_cdm30_3gauss_2nmsbin_wide_yyaxis and 230623_sh147_cdm30_speeds_logplot are the figure files for Fig. 2a, minimal media 30C.231105_sh147_cdm37_3gauss_2nmsbin_wide_yyaxis and 230623_sh147_cdm37_speeds_logplot are the figure files for Fig. 2a, minimal media 37C.231105_sh147_phmm30_3gauss_2nmsbin_wide_yyaxis and 230623_sh147_phmm30_speeds_logplot are the figure files for Fig. 2a, rich media 30C.231105_sh147_phmm37_3gauss_2nmsbin_wide_yyaxis and 230623_sh147_phmm37_speeds_logplot are the figure files for Fig. 2a, rich media 37C.230613_PBP2B_speeds_FtsZ_treadmilling_doubling_rate_SD_wide is the figure file for Fig. 2b.231213_PBP2B_speeds_FtsZ_treadmilling_constriction_rate_MEDIAN is the figure file for Fig. 2c.

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. 
Whitley_2024_Fig2_SourceData contains all the data used to produce the plots in Fig. 2.231105_sh147_cdm30_3gauss_2nmsbin_wide_yyaxis and 230623_sh147_cdm30_speeds_logplot are the figure files for Fig. 2a, minimal media 30C.231105_sh147_cdm37_3gauss_2nmsbin_wide_yyaxis and 230623_sh147_cdm37_speeds_logplot are the figure files for Fig. 2a, minimal media 37C.231105_sh147_phmm30_3gauss_2nmsbin_wide_yyaxis and 230623_sh147_phmm30_speeds_logplot are the figure files for Fig. 2a, rich media 30C.231105_sh147_phmm37_3gauss_2nmsbin_wide_yyaxis and 230623_sh147_phmm37_speeds_logplot are the figure files for Fig. 2a, rich media 37C.230613_PBP2B_speeds_FtsZ_treadmilling_doubling_rate_SD_wide is the figure file for Fig. 2b.231213_PBP2B_speeds_FtsZ_treadmilling_constriction_rate_MEDIAN is the figure file for Fig. 2c.

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. 
220405_slide2_6_MMStack_Default_561_denoise.ome_ring11 corresponds to Fig. 3a, top kymograph.220407_slide2_3_MMStack_Default_561_denoise.ome_ring18 corresponds to Fig. 3a, bottom kymograph.220928_sh147sh056_phmm_30c_vercini_slide2_561_denoise_ring3 corresponds to Fig. 3c, top kymograph.221013_sh147sh056_phmm_30c_vercini_003_561_denoise_ring1 corresponds to Fig. 3c, bottom kymograph.230613_sh147_phmm_30c_pc19_3gauss and 230607_sh147_phmm_30c_PC19_hist_logscale correspond to Fig. 3b.230613_sh147sh056_phmm_30c_3gauss and 230607_sh147_phmm_30c_G106S_hist_logscale correspond to Fig. 3d.231130_FtsZ_vs_PBP2B_speeds_95ci corresponds to Fig. 3e.Whitley_2024_Fig3_SourceData contains all data used to produce plots in Fig. 3.

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. 
220405_slide2_6_MMStack_Default_561_denoise.ome_ring11 corresponds to Fig. 3a, top kymograph.220407_slide2_3_MMStack_Default_561_denoise.ome_ring18 corresponds to Fig. 3a, bottom kymograph.220928_sh147sh056_phmm_30c_vercini_slide2_561_denoise_ring3 corresponds to Fig. 3c, top kymograph.221013_sh147sh056_phmm_30c_vercini_003_561_denoise_ring1 corresponds to Fig. 3c, bottom kymograph.230613_sh147_phmm_30c_pc19_3gauss and 230607_sh147_phmm_30c_PC19_hist_logscale correspond to Fig. 3b.230613_sh147sh056_phmm_30c_3gauss and 230607_sh147_phmm_30c_G106S_hist_logscale correspond to Fig. 3d.231130_FtsZ_vs_PBP2B_speeds_95ci corresponds to Fig. 3e.Whitley_2024_Fig3_SourceData contains all data used to produce plots in Fig. 3.

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. 220316_5_MMStack_Default_561_denoise.ome_ring14, 220316_5_MMStack_Default_561_denoise.ome_ring14_kymo, and 230607_220316_5_ring14_kymo_intensity correspond to Fig. 4a.220316_3_MMStack_Default_561_denoise.ome_ring11, 220316_3_MMStack_Default_561_denoise.ome_ring11_kymo, and 230607_220316_3_ring11_kymo_intensity correspond to Fig. 4b.220303_slide2_8_MMStack_Default_561_denoise.ome_ring22, 220303_slide2_8_MMStack_Default_561_denoise.ome_ring22_kymo, and 230607_220303_slide2_8_ring22_kymo_intensity correspond to Fig. 4c.220303_slide2_5_MMStack_Default_561_denoise.ome_ring6, 220303_slide2_5_MMStack_Default_561_denoise.ome_ring6_kymo, and 230607_220303_slide2_5_ring6_kymo_intensity correspond to Fig. 4d.

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. 220316_5_MMStack_Default_561_denoise.ome_ring14, 220316_5_MMStack_Default_561_denoise.ome_ring14_kymo, and 230607_220316_5_ring14_kymo_intensity correspond to Fig. 4a.220316_3_MMStack_Default_561_denoise.ome_ring11, 220316_3_MMStack_Default_561_denoise.ome_ring11_kymo, and 230607_220316_3_ring11_kymo_intensity correspond to Fig. 4b.220303_slide2_8_MMStack_Default_561_denoise.ome_ring22, 220303_slide2_8_MMStack_Default_561_denoise.ome_ring22_kymo, and 230607_220303_slide2_8_ring22_kymo_intensity correspond to Fig. 4c.220303_slide2_5_MMStack_Default_561_denoise.ome_ring6, 220303_slide2_5_MMStack_Default_561_denoise.ome_ring6_kymo, and 230607_220303_slide2_5_ring6_kymo_intensity correspond to Fig. 4d.

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. 2023-12-05_Morphology_SH211_005 is the raw image file behind Supplementary Figure 1a.231028_sh147_phmm_30c_100uM_iptg_0p075pct_xyl_nilered_008 is the raw image file behind Supplementary Figure 1b.231207_sh211_sh147_lengths_nilered_jgdata_231205 is the figure with data behind Supplementary Figure 1c.231208_sh147_morphology_xylose is the figure with data behind Supplementary Figure 1d.Whitley_2024_SFig1_SourceData is an excel file with cell lengths corresponding to data in SFigs 1c and 1d (one sheet for each figure panel).

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. 2023-12-05_Morphology_SH211_005 is the raw image file behind Supplementary Figure 1a.231028_sh147_phmm_30c_100uM_iptg_0p075pct_xyl_nilered_008 is the raw image file behind Supplementary Figure 1b.231207_sh211_sh147_lengths_nilered_jgdata_231205 is the figure with data behind Supplementary Figure 1c.231208_sh147_morphology_xylose is the figure with data behind Supplementary Figure 1d.Whitley_2024_SFig1_SourceData is an excel file with cell lengths corresponding to data in SFigs 1c and 1d (one sheet for each figure panel).