Automated Author Profile

Karuzina, Irina

Naucno-issledovatel'skij institut biomedicinskoj himii imeni V N Orehovica

Current S-Index

1.9

Sum of Dataset Indices for all datasets

Average Dataset Index per Dataset

0.9

Average Dataset Index per dataset

Total Datasets

2

Total datasets for this author

Average FAIR Score

65.4%

Average FAIR Score per dataset

Total Citations

0

Total citations to the author's datasets

Total Mentions

0

Total mentions of the author's datasets

S-Index Interpretation

S-Index Over Time

Cumulative Citations Over Time

Cumulative Mentions Over Time

Datasets

Identification of Cytochrome P450 2S1 in HaCaT Keratinocytes as a Responce to Surfactant Exposure

Taking the cell proteomic response to different surfactants exposure as a base of our study, we assessed the eligibility of using human HaCaT keratinocytes culture as a test-system for studying the cytotoxicity of xenobiotics. As a models of toxicants, anionic surfactant and non-ionic surfactant were chosen. Overall, 21 proteins associated with cell detoxification and xenobiotics metabolism were detected, including orphan Cytochrome P450 2S1 (CYP2S1). According to the literature data, CYP2S1 induction in HaCaT was registered only by an increase in mRNA levels. The NextProt human proteome project database also did not contain information on the presence of CYP2S1 in keratinocytes at the protein level. The acquired data on quantitative and qualitative proteomics landscape change of HaCaT culture may be used for the revealing specific proteins/metabolic pathways related to the processes of metabolism of a wide spectrum of xenobiotics. Thus, the presented results draw a new prospect of using the HaCaT keratinocytes as a model of human epidermis for studying the metabolism of drugs/toxicants in human skin in vitro.

Authors

  • Shkrigunov, Timur ;
  • Vavilov, Nikita ;
  • Samenkova, Natalia ;
  • Kisrieva, Yulia ;
  • Rusanov, Alexander ;
  • Luzgina, Natalia ;
  • Karuzina, Irina ;
  • Lisitsa, Andrey ;
  • Petushkova, Natalia
0 Citations0 Mentions65% FAIR0.9 Dataset Index
10.17632/45w5hbhpg62023

Identification of Cytochrome P450 2S1 in HaCaT Keratinocytes as a Responce to Surfactant Exposure

Taking the cell proteomic response to different surfactants exposure as a base of our study, we assessed the eligibility of using human HaCaT keratinocytes culture as a test-system for studying the cytotoxicity of xenobiotics. As a models of toxicants, anionic surfactant and non-ionic surfactant were chosen. Overall, 21 proteins associated with cell detoxification and xenobiotics metabolism were detected, including orphan Cytochrome P450 2S1 (CYP2S1). According to the literature data, CYP2S1 induction in HaCaT was registered only by an increase in mRNA levels. The NextProt human proteome project database also did not contain information on the presence of CYP2S1 in keratinocytes at the protein level. The acquired data on quantitative and qualitative proteomics landscape change of HaCaT culture may be used for the revealing specific proteins/metabolic pathways related to the processes of metabolism of a wide spectrum of xenobiotics. Thus, the presented results draw a new prospect of using the HaCaT keratinocytes as a model of human epidermis for studying the metabolism of drugs/toxicants in human skin in vitro.

Authors

  • Shkrigunov, Timur ;
  • Vavilov, Nikita ;
  • Samenkova, Natalia ;
  • Kisrieva, Yulia ;
  • Rusanov, Alexander ;
  • Luzgina, Natalia ;
  • Karuzina, Irina ;
  • Lisitsa, Andrey ;
  • Petushkova, Natalia
0 Citations0 Mentions65% FAIR0.9 Dataset Index
10.17632/45w5hbhpg6.12023