This repository contains the immunoSEQ T-cell receptor (TCR) and B-cell receptor (BCR) repertoire data used in the manuscript titled "Adaptive immune responses to Omicron BA.4/BA.5-containing bivalent mRNA booster vaccination". This data set is composed of the TCR/BCR repertoire clonotype sequences and abundances derived from human peripheral blood mononuclear cell (PBMC) samples sorted for CD4, CD8 and CD19 before and after bivalent COVID mRNA booster vaccination. In total, 84 TCR samples (CD4+/CD8+ T cell populations at pre-/post-vaccination from 21 subjects) and 42 BCR samples (CD19+ populations at at pre-/post-vaccination from 21 subjects) are present in the data set.Delmonte_TCR_data_84samples.rdsR object with the amino acid and nucleotide sequences, VDJ gene calls, and abundances of the TCR clonotypes.Delmonte_BCR_data_42samples.rds    R object with the amino acid and nucleotide sequences, VDJ gene calls, and abundances of the BCR clonotypes.Delmonte_TCR_data_compact_84samples.rdsR object with the amino acid sequences, VDJ gene calls, and abundances of the TCR clonotypes.Delmonte_BCR_data_compact_42samples.rds R object with the amino acid sequences, VDJ gene calls, and abundances of the BCR clonotypes.sampleExport_TCR.zipCompressed folder that includes the individual sample level TCR repertoire files in .tsv format.sampleExport_BCR.zipCompressed folder that includes the individual sample level BCR repertoire files in .tsv format.Funding statement: This work was supported by the Division of Intramural Research Programs of the National Institute of Allergy and Infectious Diseases and of the National Institute of Dental and Craniofacial Research, National Institutes of Health (grant AI001270).

This repository contains the immunoSEQ T-cell receptor (TCR) and B-cell receptor (BCR) repertoire data used in the manuscript titled "Adaptive immune responses to Omicron BA.4/BA.5-containing bivalent mRNA booster vaccination". This data set is composed of the TCR/BCR repertoire clonotype sequences and abundances derived from human peripheral blood mononuclear cell (PBMC) samples sorted for CD4, CD8 and CD19 before and after bivalent COVID mRNA booster vaccination. In total, 84 TCR samples (CD4+/CD8+ T cell populations at pre-/post-vaccination from 21 subjects) and 42 BCR samples (CD19+ populations at at pre-/post-vaccination from 21 subjects) are present in the data set.Delmonte_TCR_data_84samples.rdsR object with the amino acid and nucleotide sequences, VDJ gene calls, and abundances of the TCR clonotypes.Delmonte_BCR_data_42samples.rds    R object with the amino acid and nucleotide sequences, VDJ gene calls, and abundances of the BCR clonotypes.Delmonte_TCR_data_compact_84samples.rdsR object with the amino acid sequences, VDJ gene calls, and abundances of the TCR clonotypes.Delmonte_BCR_data_compact_42samples.rds R object with the amino acid sequences, VDJ gene calls, and abundances of the BCR clonotypes.sampleExport_TCR.zipCompressed folder that includes the individual sample level TCR repertoire files in .tsv format.sampleExport_BCR.zipCompressed folder that includes the individual sample level BCR repertoire files in .tsv format.Funding statement: This work was supported by the Division of Intramural Research Programs of the National Institute of Allergy and Infectious Diseases and of the National Institute of Dental and Craniofacial Research, National Institutes of Health (grant AI001270).

We describe humans with rare biallelic loss-of-function PTCRA variants impairing pre–a T cell receptor (pre-TCRa) expression. Low circulating naive ab T cell counts at birth persisted over time, with normal memory ab and high gd T cell counts. Their TCRa repertoire was biased, which suggests that noncanonical thymic differentiation pathways can rescue ab T cell development. Only a minority of these individuals were sick, with infection, lymphoproliferation, and/or autoimmunity. We also report that 1 in 4000 individuals from the Middle East and South Asia are homozygous for a common hypomorphic PTCRA variant. They had normal circulating naive ab T cell counts but high gd T cell counts. Although residual pre-TCRa expression drove the differentiation of more ab T cells, autoimmune conditions were more frequent in these patients compared with the general population.