Automated Author ProfilePing.ZHU
Department of Immunology, School of Basic Medical Sciences, Southern Medical University
Ping.ZHU
Current S-Index
Sum of Dataset Indices for all datasets
Average Dataset Index per Dataset
Average Dataset Index per dataset
Total Datasets
Total datasets for this author
Average FAIR Score
Average FAIR Score per dataset
Total Citations
Total citations to the author's datasets
Total Mentions
Total mentions of the author's datasets
S-Index Interpretation
The S-Index (Sharing Index) is a comprehensive metric that represents the cumulative impact of all your datasets. It is calculated as the sum of Dataset Index scores across all your claimed datasets.
What it means:
- A higher S-index indicates greater overall impact of your datasets relative to typical datasets in their fields of research
- The S-Index grows as you add more datasets or as existing datasets gain more citations and mentions
- It provides a single number to track your research data impact over time
Current S-Index: 0.3 (sum of 1 dataset Dataset Index scores)
More information here.
S-Index Over Time
Cumulative Citations Over Time
Cumulative Mentions Over Time
Datasets
Objective An immunological method for the detection of advanced oxidized protein products (AOPPs) was established.Methods Monoclonal antibody was prepared by oxidation modification of albumin with hypochlorous acid (HOCl) as immunogen. The specificity of monoclonal antibodies was detected by indirect, competitive ELISA and Western Blot. The stability of double antibody sandwich ELISA system was compared with that of chloramine T method.Results The monoclonal antibody (AP-4C5) reacts specifically with HOCl-oxidized proteins of different species (mouse, bovine, human, rabbit) and different species (serum albumin, serum fibrinogen, and low density lipoprotein), but does not react with unoxidized proteins. The results of Western Blot showed that AP-4C5 could bind specifically to albumin and fibrinogen with different oxidation degrees, and the bands deepened with the increase of oxidation degree. In sandwich ELISA system of AP-4C5 and 3F2, AOPPs can be specifically detected in the range of 0.25-2μg/ml (R2=0.9918). AP-4C5 and goat anti-HSA sandwich ELISA system could specifically detect 1.5 - 25μg/ml AOPP (R2=0.96875). Compared with chloramine T method, it is more suitable for detecting low concentration AOPPs.Conclusion A double-antibody sandwich ELISA method based on anti-AOPPS monoclonal antibody was developed.
Authors
- Xiaorui.HOU ;
- Ping.ZHU ;
- Yonghui.Guo ;
- Sha.Wu ;
- Beiyi.LIU