Supplementary information files for article "Characterization of a novel amidohydrolase with promiscuous esterase activity from a soil metagenomic library and its application in degradation of amide herbicides"Amide herbicides have been extensively used worldwide and have received substantial attention due to their adverse environmental effects. Here, a novel amidohydrolase gene was identified from a soil metagenomic library using diethyl terephthalate (DET) as a screening substrate. The recombinant enzyme, AmiH52, was heterologously expressed in Escherichia coli and later purified and characterised, with the highest activity occurring at 40 ℃ and pH 8.0. AmiH52 was demonstrated to have both esterase and amidohydrolase activities, which exhibited highly specific activity for p-nitrophenyl butyrate (2669 U/mg) and degrading activity against several amide herbicides. In particular, it displayed the strongest activity against propanil, with a high degradation rate of 84% at 8 h. A GC-MS analysis revealed that propanil was transformed into 3,4- dichloroaniline (3,4-DCA) during this degradation. The molecular interactions and binding stability were then analysed by molecular docking and molecular dynamics simulation, which revealed that several key amino acid residues, including Tyr164, Trp66, Ala59, Val283, Arg58, His33, His191, and His226, are involved in the specific interactions with propanil. This study provides a function-driven screening method for amide herbicide hydrolase from the metagenomic libraries and a promising propanil degrading enzyme (AmiH52) for potential applications in environmental remediation.© The Authors, CC BY 4.0

Supplementary information files for article "Characterization of a novel amidohydrolase with promiscuous esterase activity from a soil metagenomic library and its application in degradation of amide herbicides"Amide herbicides have been extensively used worldwide and have received substantial attention due to their adverse environmental effects. Here, a novel amidohydrolase gene was identified from a soil metagenomic library using diethyl terephthalate (DET) as a screening substrate. The recombinant enzyme, AmiH52, was heterologously expressed in Escherichia coli and later purified and characterised, with the highest activity occurring at 40 ℃ and pH 8.0. AmiH52 was demonstrated to have both esterase and amidohydrolase activities, which exhibited highly specific activity for p-nitrophenyl butyrate (2669 U/mg) and degrading activity against several amide herbicides. In particular, it displayed the strongest activity against propanil, with a high degradation rate of 84% at 8 h. A GC-MS analysis revealed that propanil was transformed into 3,4- dichloroaniline (3,4-DCA) during this degradation. The molecular interactions and binding stability were then analysed by molecular docking and molecular dynamics simulation, which revealed that several key amino acid residues, including Tyr164, Trp66, Ala59, Val283, Arg58, His33, His191, and His226, are involved in the specific interactions with propanil. This study provides a function-driven screening method for amide herbicide hydrolase from the metagenomic libraries and a promising propanil degrading enzyme (AmiH52) for potential applications in environmental remediation.© The Authors, CC BY 4.0

Supplementary files for article " Long-term antibiofilm efficacy of slippery covalently-attached liquid-like surfaces in dynamic and static culture conditions"

This study explores the antibiofilm potential of slippery covalently-attached liquid-like surfaces (SCALS), revealing their remarkable ability to inhibit biofilm formation over extended periods, regardless of their hydrophobic or hydrophilic nature. We engineered permanently bound liquid-like solid surfaces with exceptional slipperiness, defined by ultra-low contact angle hysteresis, and assessed their effectiveness against two nosocomial pathogens, Pseudomonas aeruginosa (PAO1) and Staphylococcus epidermidis (FH8). These surfaces achieved a 3–5 order of magnitude reduction in biofilm formation compared to polydimethylsiloxane (PDMS) under both static and dynamic culture conditions over 14 days. Impressively, both the hydrophobic and hydrophilic slippery liquid-like solid surfaces significantly outperformed widely used antimicrobial coatings containing silver particles in the long term in both static and dynamic cultures. These slippery surfaces also outperformed emerging antibiofilm surfaces like liquid-infused surfaces in extended period of dynamic cultures. We have demonstrated that ultra-low liquid-solid friction, characterized as ultra-low contact angle hysteresis, is an important predictor of the long-term antibiofilm performance of both hydrophobic and hydrophilic slippery covalently-attached liquid-like surfaces, particularly in dynamic cultures. This work elucidates the interfacial mechanisms and scientific principles underpinning the design of advanced antibiofilm surfaces capable of maintaining superior performance over the long term.

©American Chemical Society, CC BY-4.0

Supplementary files for article " Long-term antibiofilm efficacy of slippery covalently-attached liquid-like surfaces in dynamic and static culture conditions"

This study explores the antibiofilm potential of slippery covalently-attached liquid-like surfaces (SCALS), revealing their remarkable ability to inhibit biofilm formation over extended periods, regardless of their hydrophobic or hydrophilic nature. We engineered permanently bound liquid-like solid surfaces with exceptional slipperiness, defined by ultra-low contact angle hysteresis, and assessed their effectiveness against two nosocomial pathogens, Pseudomonas aeruginosa (PAO1) and Staphylococcus epidermidis (FH8). These surfaces achieved a 3–5 order of magnitude reduction in biofilm formation compared to polydimethylsiloxane (PDMS) under both static and dynamic culture conditions over 14 days. Impressively, both the hydrophobic and hydrophilic slippery liquid-like solid surfaces significantly outperformed widely used antimicrobial coatings containing silver particles in the long term in both static and dynamic cultures. These slippery surfaces also outperformed emerging antibiofilm surfaces like liquid-infused surfaces in extended period of dynamic cultures. We have demonstrated that ultra-low liquid-solid friction, characterized as ultra-low contact angle hysteresis, is an important predictor of the long-term antibiofilm performance of both hydrophobic and hydrophilic slippery covalently-attached liquid-like surfaces, particularly in dynamic cultures. This work elucidates the interfacial mechanisms and scientific principles underpinning the design of advanced antibiofilm surfaces capable of maintaining superior performance over the long term.

©American Chemical Society, CC BY-4.0