Automated Author ProfileHang.Zhang
Department of Anatomy, School of Basic Medicine, Chengde Medical CollegeDepartment of Immunology, College of Laboratory Medicine, Hebei North UniversityDepartment of Immunology, School of Basic Medicine, Chengde Medical CollegeNational Center for Human Genetic Resources, Institute of Science and Technology, NHC
Hang.Zhang
Current S-Index
Sum of Dataset Indices for all datasets
Average Dataset Index per Dataset
Average Dataset Index per dataset
Total Datasets
Total datasets for this author
Average FAIR Score
Average FAIR Score per dataset
Total Citations
Total citations to the author's datasets
Total Mentions
Total mentions of the author's datasets
S-Index Interpretation
The S-Index (Sharing Index) is a comprehensive metric that represents the cumulative impact of all your datasets. It is calculated as the sum of Dataset Index scores across all your claimed datasets.
What it means:
- A higher S-index indicates greater overall impact of your datasets relative to typical datasets in their fields of research
- The S-Index grows as you add more datasets or as existing datasets gain more citations and mentions
- It provides a single number to track your research data impact over time
Current S-Index: 1.5 (sum of 1 dataset Dataset Index scores)
More information here.
S-Index Over Time
Cumulative Citations Over Time
Cumulative Mentions Over Time
Datasets
Objective This study aims to investigate how extracellular matrix material extract affects macrophage activity, migration, phagocytosis, the expression of proinflammatory factors, and ROS production.Methods We assessed the effect of dECM extract on macrophage activity using the CCK-8 method under a light microscope. We used the Transwell migration assay to evaluate the effects of dECM extracts on the recruitment and chemotaxis of macrophages. pHrodo staining was employed to examine the impact of dECM extracts on macrophage phagocytosis. We utilized RT-qPCR and immunofluorescence staining to assess how dECM extract affects the expression of proinflammatory genes in macrophages. Additionally, we examined the expression of specific proinflammatory functional molecules. The DCFH-DA fluorescent probe method was used to detect the effect of dECM material extract on ROS production in macrophages.Results Compared with DMEM complete medium, CCK-8 results showed that dECM material extract could increase the activity of macrophages; RT-qPCR results indicated that dECM extracts could down-regulate the gene expression of proinflammatory cytokines and specific functional molecules; Flow cytometry results demonstrated that the extract of dECM material could reduce the production of ROS by macrophages. The results indicated that the dECM material extract exhibited favorable compatibility and beneficial biological properties for macrophages.
Authors
- Juwen.Ma ;
- Hang.Zhang ;
- Yunwen.Wang ;
- Yongwei.Wang ;
- Hongru.Song ;
- Yaxian.Gao