Since 2004, the Service facility SNAPO-CO2 (Service National d’Analyse des Paramètres Océaniques du CO2) housed by the LOCEAN laboratory (Paris, France) has been in charge for the analysis of Total Alkalinity (AT) and Total dissolved inorganic carbon (CT) of seawater samples on a series of cruises or ships of opportunity conducted in different regions in the frame of French and International projects. Following the first synthesis (Metzl et al, 2024, https://doi.org/10.17882/95414), 24700 new data have been quality controlled and the second version includes more than 67000 observations over 1993-2023. Sampling was performed either from CTD-Rosette casts (Niskin bottles) or collected from the ship’s seawater supply (intake at about 5m depth). After completion of each cruise, discrete samples were returned back at LOCEAN laboratory and stored in a dark room at 4 °C before analysis generally within 2-3 months after sampling (sometimes within a week). AT and CT were analyzed simultaneously by potentiometric titration using a closed cell (Edmond, 1970). Certified Reference Materials (CRMs) provided by Pr. A. Dickson (Scripps Institution of Oceanography, San Diego, USA) were used to calibrate the measurements. The same instrumentation was used for underway measurements during OISO cruises (https://campagnes.flotteoceanographique.fr/series/228/) and MINERVE cruises (https://doi.org/10.18142/128) and new AT-CT data for 2002-2021 in the Indian Ocean and Southern Ocean added in this synthesis.The second dataset is organized in one file with the format:Cruise name, Ship name, day, month, year, hour, minute, second, latitude, longitude, depth, AT (µmol/kg), Flag-AT, CT (µmol/kg), Flag-CT, Temperature (°C), Flag-Temp, Salinity (PSU), Flag-Salinity, nsample/cruise, sampling method, Version number, nsample on file.