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Automated Author Profile

Huihua.Rao

The Medical Genetic Center of Jiangxi Maternal and Child Health Hospital, Jiangxi Provincial Key Laboratory of Birth Defect for Prevention and Control

Current S-Index

2.3

Sum of Dataset Indices for all datasets

Average Dataset Index per Dataset

2.3

Average Dataset Index per dataset

Total Datasets

1

Total datasets for this author

Average FAIR Score

92.3%

Average FAIR Score per dataset

Total Citations

0

Total citations to the author's datasets

Total Mentions

0

Total mentions of the author's datasets

S-Index Interpretation

S-Index Over Time

Cumulative Citations Over Time

Cumulative Mentions Over Time

Datasets

Application of optical genome mapping in accurate detection of cryptic balanced chromosomal translocations (Version: V1)

To explore the clinical practicability of optical genome mapping in accurate detection of cryptic chromosomal balanced translocations (CBRT). A retrospective analysis was conducted on patients that visited the Jiangxi Maternal and Child Health Hospital from May 2023 to April 2024 and underwent copy number variation testing of embryos. OGM was performed on four couples with detected deletions/duplications in miscarried embryos but no abnormalities in karyotyping, and Nanopore sequencing was used for validation. OGM successfully detected four cases of CBRT, with the minimum size of translocation fragments reaching 46.86kb, of which, a t(2;6)(q37.3;q25.3) was detected using the T2T genome reference, not the GRCh38 reference. In addition, we identified four genes that were possibly disrupted by breakpoints, namely LINC01881、TRIM67、NECTIN1、CTAGE10P, which were verified by sequencing. In conclusion, optical genome mapping can accurately detect CBRT and locate the position of breakpoints. With the improvement of human reference genome and the reduction of cost, OGM will be more and more widely used in clinical practice.

Authors

  • Huihua.Rao ;
  • Wan.Lu ;
  • Jihui.Zhou ;
  • Cuiting.Wu ;
  • Huizhen.Yuan ;
  • Bicheng.Yang ;
  • Ping.Zhou
0 Citations0 Mentions92% FAIR2.3 Dataset Index
10.57760/sciencedb.o00130.05389January 2025