Urban lagoons, which provide diverse ecosystem services, exhibit variations between urban and peri-urban settings. Surveys in Tabasco evaluated public perceptions of the “La Encantada” (LE) and “Luis Gil Pérez” (LGP) lagoons, focusing on cultural and economic services. Statistical analyses of 168 respondents revealed differences based on age, gender, and education, though no significant gender or age disparities were found between the areas. Education, especially high school (75%), played a key role in shaping perceptions and influencing property ownership patterns. Despite differences in knowledge and property modifications, overall perceptions of ecosystem services remained similar. However, disparities emerged in specific landscape units, age groups, and education levels. Environmental education and recreation were highly valued, with preferences varying by age and education. Gender had no significant impact, aligning with regional trends. Education differences, influenced by cultural beliefs, shaped wetland understandings. Inconsistencies in property modifications and wetland perceptions, influenced by location, highlighted biodiversity’s importance. The perceived significance of ecosystem services aligned with folk knowledge, particularly oxygen provision. Generational differences revealed age-related perspectives on ecosystem services. The study challenged regional norms, exposing unexpected disparities in education and ecosystem service perceptions between LGP and LE. Knowledge gaps about wetlands raise conservation concerns, underscoring the need for further research. Recommendations include targeted environmental education, collaboration, and community-specific interventions. Cross-cultural exchange between LGP and LE is crucial for a comprehensive understanding of local dynamics.