The HeidelBAG unifies structural efforts of EMBL Heidelberg and the Heidelberg University Biochemistry Center (BZH). Central to all projects are large assemblies involved in chromosome organization and transcription control, in nuclear and cytoplasmic RNA regulation, in translational control by ribosome associated factors, in protein targeting and membrane protein insertion, or in post-translational and RNA modification and processing. Nearly all projects make use of integrated structural biology techniques (MX, SAXS/SANS, NMR, cryo-EM) combined with complementary biophysical and AI approaches. MX remains the essential and invariant tool for high resolution information of challenging functional modules. More and more pathological implications of the studied crucial cellular processes come into focus. In the 1st year of the continued proposal, the BAG reports on 8 publications and 25 PDB entries and many unpublished results.