Automated Author Profile

Tranfield, Erin M.

European Molecular Biology Laboratory

Current S-Index

1.5

Sum of Dataset Indices for all datasets

Average Dataset Index per Dataset

1.5

Average Dataset Index per dataset

Total Datasets

1

Total datasets for this author

Average FAIR Score

76.9%

Average FAIR Score per dataset

Total Citations

1

Total citations to the author's datasets

Total Mentions

1

Total mentions of the author's datasets

S-Index Interpretation

S-Index Over Time

Cumulative Citations Over Time

Cumulative Mentions Over Time

Datasets

Data from: Automated stitching of microtubule centerlines across serial electron tomograms (Version: 1)

Tracing microtubule centerlines in serial section electron tomography requires microtubules to be stitched across sections, that is lines from different sections need to be aligned, endpoints need to be matched at section boundaries to establish a correspondence between neighboring sections, and corresponding lines need to be connected across multiple sections. We present computational methods for these tasks: 1) An initial alignment is computed using a distance compatibility graph. 2) A fine alignment is then computed with a probabilistic variant of the iterative closest points algorithm, which we extended to handle the orientation of lines by introducing a periodic random variable to the probabilistic formulation. 3) Endpoint correspondence is established by formulating a matching problem in terms of a Markov random field and computing the best matching with belief propagation. Belief propagation is not generally guaranteed to converge to a minimum. We show how convergence can be achieved, nonetheless, with minimal manual input. In addition to stitching microtubule centerlines, the correspondence is also applied to transform and merge the electron tomograms. We applied the proposed methods to samples from the mitotic spindle in C. elegans, the meiotic spindle in X. laevis, and sub-pellicular microtubule arrays in T. brucei. The methods were able to stitch microtubules across section boundaries in good agreement with experts' opinions for the spindle samples. Results, however, were not satisfactory for the microtubule arrays. For certain experiments, such as an analysis of the spindle, the proposed methods can replace manual expert tracing and thus enable the analysis of microtubules over long distances with reasonable manual effort.

Authors

  • Weber, Britta ;
  • Tranfield, Erin M. ;
  • Höög, Johanna L. ;
  • Baum, Daniel ;
  • Antony, Claude ;
  • Hyman, Tony ;
  • Verbavatz, Jean-Marc ;
  • Prohaska, Steffen
1 Citation1 Mention77% FAIR1.5 Dataset Index
10.5061/dryad.v8j20October 2015