Polylactic acid (PLA) is the most promising bio-based alternative to traditional petrochemical plastics across diverse applications. In this study, the biodegradation performance of PLA plastic under two potential end-of-life scenarios: mesophilic and thermophilic anaerobic digestion (AD) were investigated. The biotic and abiotic influence factors were evaluated through short-time exposure experiments. The potential bacteria and archaea involved in PLA anaerobic biodegradation were identified by high-throughput 16S rRNA sequencing analysis. The results showed that PLA had different biodegradation performance at mesophilic and thermophilic digestion (the biogas yield: 36.70 ± 0.2vs 398.6 ± 1.1 mL/g VS). The increased temperature at thermophilic conditions improved the biodegradability of PLA, but an attack by microorganisms was more crucial for biodegradation. The bacteria engaged in PLA hydrolysis and acidification were closely associated with proteolytic microbes. Mesophilic biodegradation of PLA involved Clostridia (14.94%), Anaerolineae (22.6%) and acetoclastic Methanothrix (53.0%). Thermophilic biodegradation of PLA was mainly accomplished by syntrophic microbes, Clostridia (38.2%), Synergistia (18.99%) and Thermotogae (17.82%), in tandem with hydrogenotrophic Methanothermobacter (20.5%). The results provide some insights for understanding mechanisms governing PLA biodegradation under AD conditions.

Listeria monocytogenes (LM), a psychrotolerant gram-positive pathogen, poses a grave public health risk because of its capacity to persist in refrigerated food chains and withstand extreme stressors. Listeria monocytogenes employs two-component systems (TCSs), ubiquitous bacterial signaling modules comprising a histidine kinase (HK) and a response regulator (RR), to navigate environmental challenges.This study aimed to systematically elucidate the role of LiaS in acid, alkali, osmotic, oxidative stress, heavy metals, and pathogenesis and to further explore the molecular mechanism of LiaS and provide strategies for infectious diseases, the interaction between host and pathogen, and antimicrobial development.All experimental procedures were conducted in compliance with China’s Regulations for the Administration of Affairs Concerning Experimental Animals and were approved by the Zhejiang Provincial Science and Technology Department’s Institutional Animal Care and Use Committee (Permit Number: ZAFUAC2022033).

Listeria monocytogenes (LM), a psychrotolerant gram-positive pathogen, poses a grave public health risk because of its capacity to persist in refrigerated food chains and withstand extreme stressors. Listeria monocytogenes employs two-component systems (TCSs), ubiquitous bacterial signaling modules comprising a histidine kinase (HK) and a response regulator (RR), to navigate environmental challenges.This study aimed to systematically elucidate the role of LiaS in acid, alkali, osmotic, oxidative stress, heavy metals, and pathogenesis and to further explore the molecular mechanism of LiaS and provide strategies for infectious diseases, the interaction between host and pathogen, and antimicrobial development.All experimental procedures were conducted in compliance with China’s Regulations for the Administration of Affairs Concerning Experimental Animals and were approved by the Zhejiang Provincial Science and Technology Department’s Institutional Animal Care and Use Committee (Permit Number: ZAFUAC2022033).

Raw data from the study on renal tubular epithelial cell-related partial epithelial-mesenchymal transition in AAI-induced renal fibrosis via the Wnt7b/β-catenin signaling pathway.

Raw data from the study on renal tubular epithelial cell-related partial epithelial-mesenchymal transition in AAI-induced renal fibrosis via the Wnt7b/β-catenin signaling pathway.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

Polylactic acid (PLA) is the most promising bio-based alternative to traditional petrochemical plastics across diverse applications. In this study, the biodegradation performance of PLA plastic under two potential end-of-life scenarios: mesophilic and thermophilic anaerobic digestion (AD) were investigated. The biotic and abiotic influence factors were evaluated through short-time exposure experiments. The potential bacteria and archaea involved in PLA anaerobic biodegradation were identified by high-throughput 16S rRNA sequencing analysis. The results showed that PLA had different biodegradation performance at mesophilic and thermophilic digestion (the biogas yield: 36.70 ± 0.2vs 398.6 ± 1.1 mL/g VS). The increased temperature at thermophilic conditions improved the biodegradability of PLA, but an attack by microorganisms was more crucial for biodegradation. The bacteria engaged in PLA hydrolysis and acidification were closely associated with proteolytic microbes. Mesophilic biodegradation of PLA involved Clostridia (14.94%), Anaerolineae (22.6%) and acetoclastic Methanothrix (53.0%). Thermophilic biodegradation of PLA was mainly accomplished by syntrophic microbes, Clostridia (38.2%), Synergistia (18.99%) and Thermotogae (17.82%), in tandem with hydrogenotrophic Methanothermobacter (20.5%). The results provide some insights for understanding mechanisms governing PLA biodegradation under AD conditions.

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.