This dataset contains the seawater chemistry monthly means with standard deviations measured using water samples collected at the Mukilteo Research Station located in the Puget Sound, WA from 2018-08-26 to 2019-07-11. Water temperature (°C) is from the average of all 4 Durafet pH sensors deployed in the experimental tanks. Salinity data are from periodic measurements taken of all 6 experimental tanks use a hand-held Orion salinity sensor. The ambient and high-CO2 pH values are spectrophotometric pH averages from all treatment tanks. Alkalinity was calculated from the salinity values using the functional relationship in (Trigg et al., 2019). The pCO2 and calcite saturation state values were calculated using the SeaCarb R package (Gattuso et al., 2021) with the spectrophotometric pH and calculated alkalinity values as carbonate system parameter inputs. No spectrophotometric pH or Orion salinity data were recorded for January because a U.S. Government shutdown prevented collection of these data. However, automated pH and salinity sensors operating in the experimental tanks during January indicate that interpolation by averaged data from December and February, is a reasonable approximation.

This dataset contains the sample sizes and p-values for analysis of the size of juvenile Dungeness crab reared in ambient and high CO2 analysis compares the effect of CO2 within a single life-stage. The “Size Diff” is the average size of crabs rear in ambient CO2 minus the average size of crabs reared in high CO2. The “Percent Diff” is the percent difference between the ambient and high CO2 measurement.

This dataset contains a number of files related to a project that characterizes the sensitivity of the Puget Sound ecosystem to ocean acidification by estimating, at a number of taxonomic levels, the direct sensitivity of its species. It includes a database of marine species in Puget Sound (.csv) with estimates of species mineralogy in the adult and early life stages, and a list (.docx) of references called to in the database. It also includes a list (.csv) of species in the Puget Sound that are also included in a second database that collects the results of studies on the sensitivity of temperate marine species to ocean acidification (Busch and McElhany 2016, https://doi.org/10.1371/journal.pone.0160669). This latter database (.csv) is presented in an abbreviated format here including only species taxonomy, reference for the manuscript presenting data on the species, scores estimating the relevance of these studies to understanding the sensitivity of Puget Sound species to ocean acidification, and the direction of the species’ response to low pH conditions. Information on pH sensitivity from this database is rolled into scalars summarized at the family, order, and class levels (.csv) and these scalars are assigned to the species included in the Puget Sound species database.

This dataset contains output from a study designed to evaluate the impacts of ocean acidification on the food web of Puget Sound, a large estuary in the northwestern USA. An Ecopath with Ecosim model of Puget Sound was forced with scenarios designed to mimic some of the biological consequences of ocean acidification, specifically the decline in the productivity of functional groups that contain mostly calcifiers. Other functional groups in the model were allowed to respond to the scenarios in a dynamic way through indirect effects. Model output was used to calculate percent change in biomass of various model functional groups and in fisheries yield between model runs with and without ocean acidification scenarios. This information yields insight into potential changes in ecosystem services and food web structure that may occur in the Puget Sound as acidification increases.

This dataset contains laboratory experiment data that were collected to examine the effects of ocean acidification on shell condition and survival of Puget Sound pteropods. We tested whether the thecosome pteropod Limacina helicina from Puget Sound, an urbanized estuary in the northwest continental US, experiences shell dissolution and altered mortality rates when exposed to the high CO2, low aragonite saturation state (Ωa) conditions that occur in Puget Sound and the northeast Pacific Ocean. Five, week-long experiments were conducted in which we incubated pteropods collected from Puget Sound in four carbon chemistry conditions: current summer surface (~460-500 μatm CO2, Ωa ≈ 1.59), current deep water or surface conditions during upwelling (~760 and ~1600-1700 μatm CO2, Ωa ≈ 1.17 and 0.56), and future deep water or surface conditions during upwelling (~2800-3400 μatm CO2, Ωa ≈ 0.28). We measured shell condition using a scoring regime of five shell characteristics that capture different aspects of shell dissolution. We characterized carbon chemistry conditions in statistical analyses with Ωa, and conducted analyses considering Ωa both as a continuous dataset and as discrete treatments. Shell dissolution increased linearly as aragonite saturation state decreased. Discrete treatment comparisons indicate that shell dissolution was greater in undersaturated treatments compared to oversaturated treatments. Survival increased linearly with aragonite saturation state, though discrete treatment comparisons indicated that survival was similar in all but the lowest saturation state treatment. These results indicate that, under starvation conditions, pteropod survival may not be greatly affected by current and expected near-future aragonite saturation state in the NE Pacific, but shell dissolution may. Given that subsurface waters in Puget Sound’s main basin are undersaturated with respect to aragonite in the winter and can be undersaturated in the summer, the condition and persistence of the species in this estuary warrants further study.