Na⁺-K⁺-2Cl⁻ cotransporter 1 (NKCC1) is a Na⁺, K⁺, 2Cl⁻ cotransporter protein expressed widely in the nervous system. The elevated expression of NKCC1 following a stroke can result in brain edema and disruption of the blood-brain barrier (BBB), pointing out that NKCC1 may become a potential therapeutic target for improving stroke outcomes. Polygalasaponin F (PGSF) is a triterpenoid saponin isolated from Polygala japonica Houtt, which in previous studies has demonstrated neuroprotective effects by shielding neurons from excitotoxicity and exerting immunomodulatory actions, suggesting its potential as a treatment for stroke. This study aimed to observe the protective effects of PGSF on cerebral ischemia-reperfusion injury (CIRI) in rats and elucidate its positive role in CIRI through targeting NKCC1. The research findings demonstrated that rats exhibited neurological deficits and extensive cerebral infarction after cerebral ischemia-reperfusion, accompanied by elevated levels of NKCC1 mRNA and protein in brain tissue. Treatment with PGSF significantly reduced neuronal damage, infarct volume, and brain edema in rats. Both high-dose PGSF (PGSF-H) and medium-dose PGSF (PGSF-M) markedly decreased the expression levels of NKCC1 mRNA and protein. Furthermore, pharmacological modulation with 5-Aza-2-deoxycytidine (5-Aza-CDR) coupled with methylation sequencing analysis revealed that PGSF may regulated NKCC1 expression through methylation changes. Additionally, Western blotting and Evans blue extravasation experiments supported the significant inhibitory effect of PGSF on the damage of BBB. In conclusion, PGSF protected rats from the effects of CIRI, and its mechanism of action may involved PGSF-mediated DNA methylation to reduce NKCC1 gene and protein expression levels in brain tissue.

Na⁺-K⁺-2Cl⁻ cotransporter 1 (NKCC1) is a Na⁺, K⁺, 2Cl⁻ cotransporter protein expressed widely in the nervous system. The elevated expression of NKCC1 following a stroke can result in brain edema and disruption of the blood-brain barrier (BBB), pointing out that NKCC1 may become a potential therapeutic target for improving stroke outcomes. Polygalasaponin F (PGSF) is a triterpenoid saponin isolated from Polygala japonica Houtt, which in previous studies has demonstrated neuroprotective effects by shielding neurons from excitotoxicity and exerting immunomodulatory actions, suggesting its potential as a treatment for stroke. This study aimed to observe the protective effects of PGSF on cerebral ischemia-reperfusion injury (CIRI) in rats and elucidate its positive role in CIRI through targeting NKCC1. The research findings demonstrated that rats exhibited neurological deficits and extensive cerebral infarction after cerebral ischemia-reperfusion, accompanied by elevated levels of NKCC1 mRNA and protein in brain tissue. Treatment with PGSF significantly reduced neuronal damage, infarct volume, and brain edema in rats. Both high-dose PGSF (PGSF-H) and medium-dose PGSF (PGSF-M) markedly decreased the expression levels of NKCC1 mRNA and protein. Furthermore, pharmacological modulation with 5-Aza-2-deoxycytidine (5-Aza-CDR) coupled with methylation sequencing analysis revealed that PGSF may regulated NKCC1 expression through methylation changes. Additionally, Western blotting and Evans blue extravasation experiments supported the significant inhibitory effect of PGSF on the damage of BBB. In conclusion, PGSF protected rats from the effects of CIRI, and its mechanism of action may involved PGSF-mediated DNA methylation to reduce NKCC1 gene and protein expression levels in brain tissue.

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The dataset contains test and numerical data for sixteen laser-welded stainless steel I-section beam-columns. This is the world's first dataset on laser-welded stainless steel beam-columns. The test data were obtained from a laboratory testing programme on laser-welded stainless steel I-section beam-columns while the numerical data were obtained from a finite element modelling programme using ABAQUS. The geometric dimensions of the sixteen laser-welded stainless steel I-section beam-columns were also measured and are presented herein.

The dataset contains test and numerical data for sixteen laser-welded stainless steel I-section beam-columns. This is the world's first dataset on laser-welded stainless steel beam-columns. The test data were obtained from a laboratory testing programme on laser-welded stainless steel I-section beam-columns while the numerical data were obtained from a finite element modelling programme using ABAQUS. The geometric dimensions of the sixteen laser-welded stainless steel I-section beam-columns were also measured and are presented herein.

The dataset contains test and numerical data for sixteen laser-welded stainless steel I-section beam-columns. This is the world's first dataset on laser-welded stainless steel beam-columns. The test data were obtained from a laboratory testing programme on laser-welded stainless steel I-section beam-columns while the numerical data were obtained from a finite element modelling programme using ABAQUS. The geometric dimensions of the sixteen laser-welded stainless steel I-section beam-columns were also measured and are presented herein.

The dataset contains test and numerical data for sixteen laser-welded stainless steel I-section beam-columns. This is the world's first dataset on laser-welded stainless steel beam-columns. The test data were obtained from a laboratory testing programme on laser-welded stainless steel I-section beam-columns while the numerical data were obtained from a finite element modelling programme using ABAQUS. The geometric dimensions of the sixteen laser-welded stainless steel I-section beam-columns were also measured and are presented herein.

So2Sat LCZ42 is a dataset consisting of co-registered synthetic aperture radar and multispectral optical image patches acquired by the Sentinel-1 and Sentinel-2 remote sensing satellites, and the corresponding local climate zones (LCZ) label. In this version of the dataset, we included additionally the label confidence evaluation data. Ten European cities in the city list were re-labeled by having a group of remote sensing experts cast 10 independent votes on a subset of the originally labeled polygons. They were then split into geographically separated training and testing data. The latter is furthermore split randomly into testing and validation data.

The dataset contains test and numerical data for sixteen laser-welded stainless steel I-section beam-columns. This is the world's first dataset on laser-welded stainless steel beam-columns. The test data were obtained from a laboratory testing programme on laser-welded stainless steel I-section beam-columns while the numerical data were obtained from a finite element modelling programme using ABAQUS. The geometric dimensions of the sixteen laser-welded stainless steel I-section beam-columns were also measured and are presented herein.