Ginkgo biloba is one of the worlds most ancient plants, a living fossil that has remained essentially unchanged in terms of gross morphology for more than 200 million years. Representing one of the four extant gymnosperm lineages and having no living relatives, it possesses a suite of fascinating characteristics including including a large genome, outstanding resistance/tolerance to abiotic and biotic stresses, and dioecious reproduction, making it an ideal model species for biological studies.
Here we present an updated chromosome-level genome assembly using HiC technology as a major improvement of the ginkgo draft assembly. A chromosome-level reference represents a valuable resource to facilitate of studies of biologic diversity, evolutionary history, and population genetics. With technological advances, we constructed to update the existing draft assembly to the chromosome-level using Hi-C, which has been proven to be a fast, inexpensive, and accurate technology that can be applied to many species. The fresh plant leaves of two-years seedling (TM301S) were crosslinked with 1% formaldehyde. To destroy the cell wall, formaldehyde fixed powder was added to Buffer solution. The restriction endonuclease MboI was used to digest DNA, followed by biotinylated residue labeling. The Hi-C library was then sequenced on BGISEQ-500 platform with 50 bp pair-end sequencing. HiC-Pro pipeline (v2.11.1) was implemented in quality control. Of all 653,202,535 raw pair-end reads, there are 32% (207,324,555) paired Hi-C reads are valid and suitable for following analysis. Basing on these valid Hi-C reads, we used Juicer (v1.6.2) and Aiden labs Hi-C assembly pipeline (v180922) to assemble the genome with the main parameter "-m haploid -s 4 -c 12", generating 12 chromosomes spanning 9.03 Gb (~94% of the whole genome).

The African eggplant (Solanum aethiopicum) is a nutritious traditional vegetable used in many African countries, including Uganda and Nigeria. It is believed to have been domesticated in Africa from its wild relative, Solanum anguivi. S. aethiopicum has been routinely used as a source of disease resistance genes for several Solanaceae crops including Solanum melongena. Breeding of S. aethiopicum has lagged behind due to lack of genomic resources.
We assembled a 1.02 Gb draft genome of S. aethiopicum, which contained predominantly repetitive sequences (76.2%). We annotated 37,681 gene models including 34,906 protein-coding genes. We observed an expansion of disease resistance genes through two rounds of amplification of long terminal repeat retrotransposons (LTR-Rs), which may have occurred around 1.25 and 3.5 million years ago, respectively. We identified 14,995,740 SNPs by re-sequencing 65 S. aethiopicum and S. anguivi genotypes, of which 41,046 SNPs were closely linked to disease resistance genes. The domestication and demographic history analysis revealed the active selection for genes involved in drought tolerance in both Gilo and Shum groups. A pan-genome of S. aethiopicum with a total of 51,351 protein-coding genes was assembled, 7,069 genes of which are missing in the reference genome.
The genome sequence of S. aethiopicum enhances our understanding of its extraordinary biotic and abiotic resistance nature. The SNPs identified will be available for immediate use by breeders. The information provided here will greatly accelerate the selection and breeding of the African eggplant as well as other crops within the Solanaceae family.

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The domestic goat is one of the most important livestock species in the world, especially in China, India and other developing countries. Goats not only serve as an important source of meat, milk, fiber and pelts, and have fulfilled agricultural, economic, cultural and even religious roles from very early times in human civilization, but also are now used as animal models for biomedical research and transgene production of protein medicines.We would like to share all the genome data of goat. We hope the genome sequence of goat can provide a new resource for biological research and breeding of goat and other small ruminants.
We sequenced the 2.92 Gb genome to a depth of approximately 65.6 X with short reads from a series of libraries with various insert sizes ( 170 bp, 350 bp, 800 bp, 2 kb, 5 kb, 10 kb and 20 kb) on a HiSeq 2000 sequencer.
The assembled scaffolds of high quality sequences total 191.5 Gb, with the contig and scaffold N50 values of 18.7 kb and 2.21 Mb respectively. We identified 22,175 protein-coding genes.In addition, we also provide the restriction-enzyme fragment maps derived from the whole genome mapping (WGM) technology developed by the Argus System (method described in this paper).
Scaffolds derived from de novo assembly of next-generation sequencing data are converted into restriction maps by in silico restriction enzyme digestion. Then, the distance between restriction enzyme sites in the sequencing-derived scaffolds are matched to the lengths of the optical fragments in the single-molecule WGM restriction maps. Matches allow the scaffolds to be extended and linked into super-scaffolds.

The Plum (Prunus mume), was domesticated in China more than 3,000 years ago as ornamental plant and fruit, is one of the first genomes among Prunus subfamilies of Rosaceae to be sequenced.
DNA from the plum was collected in Tongmai town, Tibet, China. We sequenced the genome to a depth of approximately 101.4 X with short reads from a series of libraries with various insert sizes ( 180bp, 500bp, 800bp, 2kb, 5kb, 10kb, 20kb and 40kb) on a HiSeq 2000 sequencer.
The assembled scaffolds of high quality sequences total 28.4 Gb with the contig and scaffold N50 values of 31.8 kb and 577.8 kb respectively.

Here we present the genome of the pigeonpea (Cajanus cajan), a widely farmed diploid legume species. It is an important reference genome for food crop development as many crop species, such as soybean (Glycine max), chickpea (Cicer arietinum), lentil (Lens culinaris), and alfalfa (Medicago sativa), are legumes. The genetic improvement of pigeonpea has ramifications for food protection as well, as it is cultivated primarily in small-scale holdings in semi-arid tropical regions of the developing world.The 237.2 Gb of sequence were generated using the Illumina next-generation sequencing platform to generate. Scaffolds representing 72.7% (605.78 Mb) of the 833.07-Mb pigeonpea genome were assembled using the Illumina sequence and Sanger-based bacterial artificial chromosome end sequences and a genetic map. A few segmental duplication events, but no recent genome-wide duplication events, are observable.

Chinese hamster ovary (CHO) K1 cells are a cell line cultured from the ovary of the Chinese hamster (Cricetulus griseus). CHO cells are often used in biological and medical studies and commercially in the production of therapeutic proteins, which contribute significantly to the $100 billion biopharmaceutical market.BGI sequenced the CHO K1 genome genome using next-generation sequencing technology, assembling a 2.45G genome with 24,383 predicted genes.