Automated Author Profile

Liu, Xian Shuang

Current S-Index

1.0

Sum of Dataset Indices for all datasets

Average Dataset Index per Dataset

0.5

Average Dataset Index per dataset

Total Datasets

2

Total datasets for this author

Average FAIR Score

84.6%

Average FAIR Score per dataset

Total Citations

0

Total citations to the author's datasets

Total Mentions

0

Total mentions of the author's datasets

S-Index Interpretation

S-Index Over Time

Cumulative Citations Over Time

Cumulative Mentions Over Time

Datasets

Identification of miRNomes associated with adult neurogenesis after stroke using Argonaute 2-based RNA sequencing

Neurogenesis is associated with functional recovery after stroke. However, the underlying molecular mechanisms have not been fully investigated. Using an Ago2-based RNA immunoprecipitation to immunoprecipated Ago2-RNA complexes followed by RNA sequencing (Ago2 RIP-seq) approach, we profiled the miRNomes in neural progenitor cells (NPCs) harvested from the subventricular zone (SVZ) of the lateral ventricles of young adult rats. We identified more than 7 and 15 million reads in normal and ischemic NPC libraries, respectively. We found that stroke substantially changed Ago2-associated miRNA profiles in NPCs compared to those in non-ischemic NPCs. We also discovered a new complex repertoire of isomiRs and multiple miRNA-miRNA* pairs and numerous novel miRNAs in the non-ischemic and ischemic NPCs. Among them, pc-3p-17172 significantly regulated NPC proliferation and neuronal differentiation. Collectively, the present study reveals profiles of Ago2-associated miRNomes in non-ischemic and ischemic NPCs, which provide a molecular basis to further investigate the role of miRNAs in mediating adult neurogenesis under physiological and ischemic conditions.

Authors

  • Liu, Xian Shuang ;
  • Fan, Bao Yan ;
  • Pan, Wan Long ;
  • Li, Chao ;
  • Levin, Albert M. ;
  • Xinli Wang ;
  • Zhang, Rui Lan ;
  • Zervos, Thomas M. ;
  • Jiani Hu ;
  • Zhang, Xiao Ming ;
  • Chopp, Michael ;
  • Zhang, Zheng Gang
0 Citations0 Mentions85% FAIR0.5 Dataset Index
10.6084/m9.figshare.3443318.v12016

Identification of miRNomes associated with adult neurogenesis after stroke using Argonaute 2-based RNA sequencing

Neurogenesis is associated with functional recovery after stroke. However, the underlying molecular mechanisms have not been fully investigated. Using an Ago2-based RNA immunoprecipitation to immunoprecipated Ago2-RNA complexes followed by RNA sequencing (Ago2 RIP-seq) approach, we profiled the miRNomes in neural progenitor cells (NPCs) harvested from the subventricular zone (SVZ) of the lateral ventricles of young adult rats. We identified more than 7 and 15 million reads in normal and ischemic NPC libraries, respectively. We found that stroke substantially changed Ago2-associated miRNA profiles in NPCs compared to those in non-ischemic NPCs. We also discovered a new complex repertoire of isomiRs and multiple miRNA-miRNA* pairs and numerous novel miRNAs in the non-ischemic and ischemic NPCs. Among them, pc-3p-17172 significantly regulated NPC proliferation and neuronal differentiation. Collectively, the present study reveals profiles of Ago2-associated miRNomes in non-ischemic and ischemic NPCs, which provide a molecular basis to further investigate the role of miRNAs in mediating adult neurogenesis under physiological and ischemic conditions.

Authors

  • Liu, Xian Shuang ;
  • Fan, Bao Yan ;
  • Pan, Wan Long ;
  • Li, Chao ;
  • Levin, Albert M. ;
  • Xinli Wang ;
  • Zhang, Rui Lan ;
  • Zervos, Thomas M. ;
  • Jiani Hu ;
  • Zhang, Xiao Ming ;
  • Chopp, Michael ;
  • Zhang, Zheng Gang
0 Citations0 Mentions85% FAIR0.5 Dataset Index
10.6084/m9.figshare.34433182016