ABSTRACT In the last few years, an increasing number of debilitated Magellanic penguins (Spheniscus magellanicus) has been rescued and taken to rehabilitation centers on Brazil’s southern coast to be clinically treated and evaluated for re-introduction. This work aims to compare the viability of heparinized plasma with the viability of serum for biochemistry analyses under rehabilitation conditions. Blood sampled from 31 physically healthy rescued penguins was processed into serum/plasma-paired samples and analyzed for 12 biochemical parameters: alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), cholesterol (CHOL), creatine kinase (CK), gamma-glutamyl transpeptidase (GGT), glucose, (GLU) lactate dehydrogenase (LDH), total proteins (TP), triglycerides (TG), urea (UR), and uric acid (UA).The results showed that six paired samples presented visual signs of hemolysis (visual hemolytic score≥1), four of which occurred exclusively in the serum counterpart. Significant differences (P≤ 0.5) between sample types were found for CHOL (3%), GLU (6%) and TG (52%). Only TG was considered clinically relevant (>10%). All mean/median results fell within the available reference intervals by the Association of Zoos and Aquariums (Penguin, 2014). In conclusion, we verified that heparinized plasma is a viable sample for the clinical biochemistry of rescued Magellanic penguins as it yields compatible results with serum, while providing practical benefits. The adoption of this practice favors a faster bird recovery, by minimizing blood sampling volume, and optimizes material resources, allowing use of the same collector tube as for hematology.

ABSTRACT In the last few years, an increasing number of debilitated Magellanic penguins (Spheniscus magellanicus) has been rescued and taken to rehabilitation centers on Brazil’s southern coast to be clinically treated and evaluated for re-introduction. This work aims to compare the viability of heparinized plasma with the viability of serum for biochemistry analyses under rehabilitation conditions. Blood sampled from 31 physically healthy rescued penguins was processed into serum/plasma-paired samples and analyzed for 12 biochemical parameters: alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), cholesterol (CHOL), creatine kinase (CK), gamma-glutamyl transpeptidase (GGT), glucose, (GLU) lactate dehydrogenase (LDH), total proteins (TP), triglycerides (TG), urea (UR), and uric acid (UA).The results showed that six paired samples presented visual signs of hemolysis (visual hemolytic score≥1), four of which occurred exclusively in the serum counterpart. Significant differences (P≤ 0.5) between sample types were found for CHOL (3%), GLU (6%) and TG (52%). Only TG was considered clinically relevant (>10%). All mean/median results fell within the available reference intervals by the Association of Zoos and Aquariums (Penguin, 2014). In conclusion, we verified that heparinized plasma is a viable sample for the clinical biochemistry of rescued Magellanic penguins as it yields compatible results with serum, while providing practical benefits. The adoption of this practice favors a faster bird recovery, by minimizing blood sampling volume, and optimizes material resources, allowing use of the same collector tube as for hematology.

ABSTRACT This report describes the case of a mare, of the Campeiro breed, used as an embryo donor, which had recurrent cystitis and urinary incontinence crisis. Clinical signs evolved to progressive weight loss, anorexia, apathy, and isolation from the group. Physical examination showed tail hypotonia, perineal hypalgesia, rectal and bladder sagging compatible with signs related to cauda equina syndrome. Complementary laboratory and sonographic assessment, and necropsy confirmed the diagnosis of chronic renal failure (CRF), which was attributed to the ascending pyelonephritis. The examination of urine culture showed growth of bacteria of the genus Streptococcus sp. This is a rare case in the equine species where the lower motor neuron dysfunction led the development of infectious process in the urinary tract, progressing to renal chronic condition incompatible with life.

ABSTRACT This report describes the case of a mare, of the Campeiro breed, used as an embryo donor, which had recurrent cystitis and urinary incontinence crisis. Clinical signs evolved to progressive weight loss, anorexia, apathy, and isolation from the group. Physical examination showed tail hypotonia, perineal hypalgesia, rectal and bladder sagging compatible with signs related to cauda equina syndrome. Complementary laboratory and sonographic assessment, and necropsy confirmed the diagnosis of chronic renal failure (CRF), which was attributed to the ascending pyelonephritis. The examination of urine culture showed growth of bacteria of the genus Streptococcus sp. This is a rare case in the equine species where the lower motor neuron dysfunction led the development of infectious process in the urinary tract, progressing to renal chronic condition incompatible with life.

This work integrates the available geological information and geochronology data for the Cretaceous–Recent magmatism in the South Atlantic, represented by onshore and offshore magmatic events, including the oceanic islands along the transform faults and near the mid-ocean ridge. The analysis of the igneous rocks and their tectonic settings allows new insights into the evolution of the African and Brazilian continental margins during the South Atlantic opening. Following the abundant volcanism in the Early Cretaceous, the magmatic quiescence during the Aptian–Albian times is a common characteristic of almost all Brazilian and West African marginal basins. However, rocks ascribed to the Cabo Granite (104 Ma) are observed in NE Brazil. In West Africa, sparse Aptian–Albian ages are observed in a few coastal igneous centres. In the SE Brazilian margin, an east–west alkaline magmatic trend is observed from Poços de Caldas to Cabo Frio, comprising igneous intrusions dated from 87 to 64 Ma. Mafic dyke swarms trending NW also occur in the region extending from the Cabo Frio Province towards the Central Brazilian Craton. On the West African side, Early Cretaceous–Recent volcanism is observed in the Walvis Ridge (139 Ma), the St Helena Ridge (81 Ma) and the Cameroon Volcanic Line (Early Tertiary–Recent). Volcanic islands such as Ascencion (1.0–0.65 Ma), Tristão da Cunha (2.5–0.13 Ma) and the St Helena islands (12 Ma) most probably correspond to mantle plumes or hot spots presently located near the mid-Atlantic spreading centre. Within the South America platform and deep oceanic regions, the following volcanic islands are observed: the Rio Grande Rise (88–86 Ma), Abrolhos (54–44 Ma), the Vitória–Trindade Chain (no age), Trindade (2.8–1.2 Ma) and Fernando de Noronha (12–1.5 Ma). There are several volcanic features along the NW–SE-trending Cruzeiro do Sul Lineament from Cabo Frio to the Rio Grande Rise, but they have not been dated. The only known occurrence of serpentinized mantle rocks in the South Atlantic margin is associated with the Saint Peter and Saint Paul Rocks located along the São Paulo Fracture Zone. The Cameroon Volcanic Line in NW Africa is related to the magmatism that started in the Late Cretaceous and shows local manifestations up to the Present. The compilation of all available magmatic ages suggests an asymmetrical evolution between the African and South America platforms with more pre-break-up and post-break-up magmatism observed in the Brazilian margin. This is most likely to have resulted from the different geological processes operating during the South Atlantic Ocean opening, shifts in the spreading centre, and, possibly, the rising and waning of mantle plumes.

This work integrates the available geological information and geochronology data for the Cretaceous–Recent magmatism in the South Atlantic, represented by onshore and offshore magmatic events, including the oceanic islands along the transform faults and near the mid-ocean ridge. The analysis of the igneous rocks and their tectonic settings allows new insights into the evolution of the African and Brazilian continental margins during the South Atlantic opening. Following the abundant volcanism in the Early Cretaceous, the magmatic quiescence during the Aptian–Albian times is a common characteristic of almost all Brazilian and West African marginal basins. However, rocks ascribed to the Cabo Granite (104 Ma) are observed in NE Brazil. In West Africa, sparse Aptian–Albian ages are observed in a few coastal igneous centres. In the SE Brazilian margin, an east–west alkaline magmatic trend is observed from Poços de Caldas to Cabo Frio, comprising igneous intrusions dated from 87 to 64 Ma. Mafic dyke swarms trending NW also occur in the region extending from the Cabo Frio Province towards the Central Brazilian Craton. On the West African side, Early Cretaceous–Recent volcanism is observed in the Walvis Ridge (139 Ma), the St Helena Ridge (81 Ma) and the Cameroon Volcanic Line (Early Tertiary–Recent). Volcanic islands such as Ascencion (1.0–0.65 Ma), Tristão da Cunha (2.5–0.13 Ma) and the St Helena islands (12 Ma) most probably correspond to mantle plumes or hot spots presently located near the mid-Atlantic spreading centre. Within the South America platform and deep oceanic regions, the following volcanic islands are observed: the Rio Grande Rise (88–86 Ma), Abrolhos (54–44 Ma), the Vitória–Trindade Chain (no age), Trindade (2.8–1.2 Ma) and Fernando de Noronha (12–1.5 Ma). There are several volcanic features along the NW–SE-trending Cruzeiro do Sul Lineament from Cabo Frio to the Rio Grande Rise, but they have not been dated. The only known occurrence of serpentinized mantle rocks in the South Atlantic margin is associated with the Saint Peter and Saint Paul Rocks located along the São Paulo Fracture Zone. The Cameroon Volcanic Line in NW Africa is related to the magmatism that started in the Late Cretaceous and shows local manifestations up to the Present. The compilation of all available magmatic ages suggests an asymmetrical evolution between the African and South America platforms with more pre-break-up and post-break-up magmatism observed in the Brazilian margin. This is most likely to have resulted from the different geological processes operating during the South Atlantic Ocean opening, shifts in the spreading centre, and, possibly, the rising and waning of mantle plumes.