Additional file 4. Excel file tabulating: presence/absence of signal peptides and transmembrane domains in N. vectensis trypsins, amino acid sequences for trypsin catalytic domains for all taxa, pfam IDs for all domains, and a summary of single-cell expression of trypsins published previously [16].

Additional file 4. Excel file tabulating: presence/absence of signal peptides and transmembrane domains in N. vectensis trypsins, amino acid sequences for trypsin catalytic domains for all taxa, pfam IDs for all domains, and a summary of single-cell expression of trypsins published previously [16].

Additional file 7. Trypsin protein IDs from all taxa examined in this study.

Additional file 7. Trypsin protein IDs from all taxa examined in this study.

Summary of everything. Sheet 1: Stress Gene Survey. List of tissue-specific targets and their reciprocal best blast hit (accession ID) from the dataset presented by Reitzel et al [63]. Included fields: Tissue, unique transcript ID (NvecRef ID), reciprocal best blastx hit (Hit), stressor type (pathogen, wound, or chemical). Transcripts with reciprocal best blast hits involved in both pathogen and wound stress are indicated as: Pathogen.Wound. Sheet 2: Transcriptome Summary. Summary of all 17,313 transcripts used for differential expression and orthology analyses. Each entry contains the following: unique transcriptome ID (NvecRef ID), transcript length (in nucleotides), orthology group ID (GrID): N – Nematostella-specific, E – Edwarsiidae-specific, A – anthozoan-specific, C – cnidarian-specific, M – conserved/metazoan, number of tissues (out of three) in which the transcript is expressed, differential expression result (UP/mes is upregulated in the mesenteries relative to both other tissues), mean counts across all tissues (AllMean) and across replicates within a tissue (MesMean, NemMean, TenMean), log2 fold change for all pairwise tissue comparisons (negative values indicate upregulation in the second tissue), adjusted p-values/false discovery rates for all pairwise comparisons, and raw counts for each tissue replicate. Sheet 3: Alien Index Results. Fields: unique transcriptome ID (NvecRef ID), alien index (AI), % identity and coverage of the top blast hit, and whether the sequence was deemed present in the genome (http://genome.jgi.doe.gov/Nemve1/Nemve1.home.html). Sheet 4: Primers. Sequences for all primers used in qPCR and standard PCR analyses. Primer sequences for each transcript are presented 5’ → 3’. (XLSX 4037 kb)

Summary of everything. Sheet 1: Stress Gene Survey. List of tissue-specific targets and their reciprocal best blast hit (accession ID) from the dataset presented by Reitzel et al [63]. Included fields: Tissue, unique transcript ID (NvecRef ID), reciprocal best blastx hit (Hit), stressor type (pathogen, wound, or chemical). Transcripts with reciprocal best blast hits involved in both pathogen and wound stress are indicated as: Pathogen.Wound. Sheet 2: Transcriptome Summary. Summary of all 17,313 transcripts used for differential expression and orthology analyses. Each entry contains the following: unique transcriptome ID (NvecRef ID), transcript length (in nucleotides), orthology group ID (GrID): N – Nematostella-specific, E – Edwarsiidae-specific, A – anthozoan-specific, C – cnidarian-specific, M – conserved/metazoan, number of tissues (out of three) in which the transcript is expressed, differential expression result (UP/mes is upregulated in the mesenteries relative to both other tissues), mean counts across all tissues (AllMean) and across replicates within a tissue (MesMean, NemMean, TenMean), log2 fold change for all pairwise tissue comparisons (negative values indicate upregulation in the second tissue), adjusted p-values/false discovery rates for all pairwise comparisons, and raw counts for each tissue replicate. Sheet 3: Alien Index Results. Fields: unique transcriptome ID (NvecRef ID), alien index (AI), % identity and coverage of the top blast hit, and whether the sequence was deemed present in the genome (http://genome.jgi.doe.gov/Nemve1/Nemve1.home.html). Sheet 4: Primers. Sequences for all primers used in qPCR and standard PCR analyses. Primer sequences for each transcript are presented 5’ → 3’. (XLSX 4037 kb)

Commands for bioinformatic analyses and statistical methods. (TXT 5 kb)

(A) Small cellular material moving through the tentacle lumen of a primary/immature polyp. (B) Small cellular material and nematosomes moving through the tentacle of an adult polyp. (ZIP 51824 kb)

Full GO annotation reports for upregulated transcripts by tissue. Sheet 1 (summary) includes full Blast2GO reports for all three tissues. Abbreviations: Mes – mesenteries, Nem – nematosomes, Ten – tentacles, Gene Symbol – NCBI approved gene symbol, GO ID – gene ontology identifier, BP (GO category) – Biological process, CC – cellular component, MF – molecular function, TrID – transcript ID (NvecRef transcriptome), HumRef – accession number (human orthologs) for NCBI’s Refseq database. (XLSX 183 kb)

(A) Small cellular material moving through the tentacle lumen of a primary/immature polyp. (B) Small cellular material and nematosomes moving through the tentacle of an adult polyp. (ZIP 51824 kb)