Sequence, primer concentration ([Primer]), annealing temperature (T°), total number of alleles, and size range information for nine microsatellite markers previously designed for Cimex lectularius (by 1: Fountain et al. [41] and 2: Booth et al. [42]), used here on Cimex adjunctus samples. For each marker, we also show the average number of alleles (NA), expected heterozygosity (HE), observed heterozygosity (HO) and inbreeding coefficient (GIS) across all sites with five or more sampled individuals. (XLSX 51 kb)

Details of sampling locations where we collected Cimex adjunctus specimens used in this study. Site refers to each unique sampling location and correspond to those in Additional file 1: Table S1. Sample size refers to the number of specimens collected at each site. Capture method refers to the way C. adjunctus samples were collected: either from the body of a bat that was captured outside a roost with a mist net or a harp trap (Bat capture), or from inside of the roost itself (Roost visit). Site characteristics refers either to the type of roost (house, barn, cabin, church or abandoned mine) in, or next to which, we collected samples, or to the forested land jurisdiction where collection was performed. (XLSX 37 kb)

List of Cimex adjunctus specimens included in analyses. Site refers to each unique sampling location in the study. Host species refers to the bat species from which the samples were collected, or which was inhabiting the roost from which the samples were collected (EPFU : Eptesicus fuscus, MYLU : Myotis lucifugus, MYSE : Myotis septentrionalis). Individuals with the same haplotype number share the same mitochondrial CO1 haplotype sequence (we could not obtain CO1 information for six individuals). We provide the Genbank accession number for each unique haplotype the first time it appears in the table. The genetic cluster (identified by Geneland based on microsatellite data) to which each specimen was assigned is also given (we could not obtain microsatellite data for 10 individuals). (XLSX 51 kb)

Genotypic data at 9 microsatellite markers for 150 Cimex adjunctus individuals (we could not obtain microsatellite data for 10 individuals). The first three digits in each string describe the length (in base pairs) of the first allele, and the last three describe the length of the second allele. Missing data is identified with â 000000â . (XLSX 59 kb)

Host species and sampling year from which C. adjunctus samples assigned to each genetic cluster were collected. (XLSX 41 kb)

Host species and sampling year from which C. adjunctus samples assigned to each genetic cluster were collected. (XLSX 41 kb)

Genotypic data at 9 microsatellite markers for 150 Cimex adjunctus individuals (we could not obtain microsatellite data for 10 individuals). The first three digits in each string describe the length (in base pairs) of the first allele, and the last three describe the length of the second allele. Missing data is identified with â 000000â . (XLSX 59 kb)

List of Cimex adjunctus specimens included in analyses. Site refers to each unique sampling location in the study. Host species refers to the bat species from which the samples were collected, or which was inhabiting the roost from which the samples were collected (EPFU : Eptesicus fuscus, MYLU : Myotis lucifugus, MYSE : Myotis septentrionalis). Individuals with the same haplotype number share the same mitochondrial CO1 haplotype sequence (we could not obtain CO1 information for six individuals). We provide the Genbank accession number for each unique haplotype the first time it appears in the table. The genetic cluster (identified by Geneland based on microsatellite data) to which each specimen was assigned is also given (we could not obtain microsatellite data for 10 individuals). (XLSX 51 kb)

Details of sampling locations where we collected Cimex adjunctus specimens used in this study. Site refers to each unique sampling location and correspond to those in Additional file 1: Table S1. Sample size refers to the number of specimens collected at each site. Capture method refers to the way C. adjunctus samples were collected: either from the body of a bat that was captured outside a roost with a mist net or a harp trap (Bat capture), or from inside of the roost itself (Roost visit). Site characteristics refers either to the type of roost (house, barn, cabin, church or abandoned mine) in, or next to which, we collected samples, or to the forested land jurisdiction where collection was performed. (XLSX 37 kb)

Sequence, primer concentration ([Primer]), annealing temperature (T°), total number of alleles, and size range information for nine microsatellite markers previously designed for Cimex lectularius (by 1: Fountain et al. [41] and 2: Booth et al. [42]), used here on Cimex adjunctus samples. For each marker, we also show the average number of alleles (NA), expected heterozygosity (HE), observed heterozygosity (HO) and inbreeding coefficient (GIS) across all sites with five or more sampled individuals. (XLSX 51 kb)