Automated Author Profile

Studencka, M.

Current S-Index

1.4

Sum of Dataset Indices for all datasets

Average Dataset Index per Dataset

0.7

Average Dataset Index per dataset

Total Datasets

2

Total datasets for this author

Average FAIR Score

84.6%

Average FAIR Score per dataset

Total Citations

1

Total citations to the author's datasets

Total Mentions

0

Total mentions of the author's datasets

S-Index Interpretation

S-Index Over Time

Cumulative Citations Over Time

Cumulative Mentions Over Time

Datasets

Supplementary Material for: <i>Ccdc33, </i>a Predominantly Testis-Expressed Gene, Encodes a Putative Peroxisomal Protein

Many genes crucial for male fertility are often predominantly or exclusively expressed in male germ cells. The analysis of mouse models has demonstrated the functional importance of peroxisomes in spermatogenesis. The CCDC33 protein has been reported to be a cancer/testis (CT) antigen. We found that mouse Ccdc33 is predominantly expressed in the testis and undergoes alternative splicing to produce at least 4 different transcripts. The protein encoded by Ccdc33 contains 3 coiled-coil domains, a C2-domain, 2 ER membrane retention signal-like motifs and 2 putative peroxisomal targeting signals type 2 (PTS2). We could demonstrate that the second PTS2 sequence is functional and responsible for the targeting of CCDC33 to peroxisomes. Moreover, in HeLa cells CCDC33-dsRED fusion protein co-localized with a known peroxisomal protein, namely PXT1, and showed punctuate intracellular distribution. Taken together, the mouse Ccdc33 encodes a putative peroxisomal protein and is predominantly expressed in male germ cells. The expression starts at the primary spermatocyte stage, suggesting an important role of this protein during spermatogenesis.

Authors

  • Kaczmarek, K. ;
  • Niedzialkowska, E. ;
  • Studencka, M. ;
  • Schulz, Y. ;
  • Grzmil, P.
1 Citation0 Mentions85% FAIR0.9 Dataset Index
10.6084/m9.figshare.51208752010

Supplementary Material for: <i>Ccdc33, </i>a Predominantly Testis-Expressed Gene, Encodes a Putative Peroxisomal Protein

Many genes crucial for male fertility are often predominantly or exclusively expressed in male germ cells. The analysis of mouse models has demonstrated the functional importance of peroxisomes in spermatogenesis. The CCDC33 protein has been reported to be a cancer/testis (CT) antigen. We found that mouse Ccdc33 is predominantly expressed in the testis and undergoes alternative splicing to produce at least 4 different transcripts. The protein encoded by Ccdc33 contains 3 coiled-coil domains, a C2-domain, 2 ER membrane retention signal-like motifs and 2 putative peroxisomal targeting signals type 2 (PTS2). We could demonstrate that the second PTS2 sequence is functional and responsible for the targeting of CCDC33 to peroxisomes. Moreover, in HeLa cells CCDC33-dsRED fusion protein co-localized with a known peroxisomal protein, namely PXT1, and showed punctuate intracellular distribution. Taken together, the mouse Ccdc33 encodes a putative peroxisomal protein and is predominantly expressed in male germ cells. The expression starts at the primary spermatocyte stage, suggesting an important role of this protein during spermatogenesis.

Authors

  • Kaczmarek, K. ;
  • Niedzialkowska, E. ;
  • Studencka, M. ;
  • Schulz, Y. ;
  • Grzmil, P.
0 Citations0 Mentions85% FAIR0.5 Dataset Index
10.6084/m9.figshare.5120875.v12010