Phagocyosis Assay, normalized to controls

RAW 264.7 cells were treated with welding fume suspensions for 3 or 6 h or cytochalasin D for 3 h. Cells were washed, pHrodo Red E. coli BioParticles were added for 2 h, and plates were read to measure changes in fluorescence. All values were normalized to PBS controls (=100% phagocytosis). Values represent means from 3 replicate wells per condition.