RNA seq data of bovine mammary epithelial cells upon IFN-γ stimulation

BMECs were treated with or without IFN-γ (10 ng/ mL) (Kingfisher Biotech, St. Paul, Brazil, RP0013B-100) for 24 h. Total RNA was extracted from the control BMECs (NC) and IFN-γ-treated the fifth generation BMECs (N05) and IFN-γ-treated the 20th generation BMECs (N20). The cells were washed with PBS for three times after discarded the supernatant. According to the instructions, the cells were lysed by RNAiso plus (TaKaRa, Japan) and placed at room temperature for 5 min, and chloroform was added in. After centrifugation at 12000 g, the supernatant was taken out and mixed with isopropyl alcohol in equal volume and then waited for 10 min and centrifuged. The precipitate was collected and washed with 75% ethanol, stored at − 80 °C as the total RNA. RNA-seq analysis was performed only on samples that met the manufacturer’s standards. RNA was sequenced using Illumina HiSeqTM 4000. Finally, the differentially expressed genes screened out.