Raw dataset for bacterial exopolysaccharide production using riceberry broken rice as precursors by Bacillus spp. isolated from Thai milk kefir with its antioxidant and antimicrobial capacities

This raw dataset reflects an analysis of the antioxidant and antibacterial activity of exopolysaccharide (EPS) produced by seven Bacillus spp. isolated from Thai milk kefir [1] on a substrate of riceberry broken rice. Riceberry broken rice was analyzed for its moisture, ash, fat, protein, fiber, starch, sugar, hemicellulose, and cellulose plus lignin content when used as an EPS-producing substrate. After 3 days of fermentation, dataset on the total plate count, the reducing sugar content, the pH of the culture, and the fresh and dry weight of the EPS was recorded. In response to the EPS produced by various Bacillus spp., the total phenolic content, total flavonoid content, antimicrobial activity against Streptococcus agalactiae and Staphylococcus aureus, and hydroxyl radical scavenging activity were collected. Agar disk diffusion, spectrophotometry for antioxidant activity and bioactive content, proximate analysis, the dinitrosalicylic acid method, pH meter readings, the total plate count via serial dilutions, and the spread plate technique were the primary techniques used.