Published on 02 May 2024
Raw ONT pod5 data: Barcoded D2O Aminobacter P9b DNA
View DatasetHöner zu Siederdissen, Christian;Spangenberg, Jannes;Taubert, Martin;Marz, Manja
Description
Aminobacter P9b samples were incubated on a 100% D2O, 30% D2O and 0% D2O medium, respectively. Cells were harvested by centrifugation. Pellets were resuspended and DNA extracted. The DNA was barcoded using the ONTs native barcoding kit, prepared with the SQK-LSK109 protocol, and sequenced on a MinION with an r9.4.1 flowcell.For barcoding, we used the EXP-NBD114 Barcodes 14, 15, and 16:BC 14: 0% D2OBC 15: 30% D2OBC 16: 100% D2O
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Publication Details
Subfield
Molecular Biology
Field
Biochemistry, Genetics and Molecular Biology
Domain
Life Sciences
Confidence Score
39%
Source
Scholar Data Model