Development and optimization of species-specific qPCR assays for eDNA detection of the mucket (Ortamannia ligamentina) and spectaclecase (Cumberlandia monodonta) mussels’ male mitotype

Katy E Klymus;Dannise V Ruiz Ramos;Nathan L Thompson

Description

This dataset describes the sensitivity (limit of detection and limit of quantification) and specificity (list of species tested against the assays) of two qPCR assays designed to detect the male mitotype of two freshwater mussel species Ortmanniana ligamentina and Cumberlandia monodonta from water samples (eDNA).

Citations (0)

Mentions (0)

Metrics

Dataset Index

0.3

FAIR Score

46%

Citations

0

Mentions

0

Metrics Over Time

Publication Details

DOI

Publisher

U.S. Geological Survey

License

Creative Commons Zero v1.0 Universal

Assigned Domain

Subfield

Ecology

Field

Environmental Science

Domain

Physical Sciences

Confidence Score

46%

Source

Scholar Data Model

Keywords

biotapolymerase chain reactionmollusksGeneticsFOS: Biological sciencesenvironmental DNA

Normalization Factors

FT

56.73

CTw

1.00

MTw

1.00